Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bej.2020.107694 http://hdl.handle.net/11449/199058 |
Resumo: | Fungi bioproducts have recently become commercially important to the food and pharmaceutical industry. However, different structural forms exhibited by filamentous fungi throughout their life cycles are often a bottleneck for productivity in many industrial processes. In this work, the relationship among mycelium accumulation, morphology, and colorant production was evaluated. Experiments were performed in an incubator shaker and in a stirred-tank bioreactor. First, a series of nitrogen sources and pure vitamins were investigated as additives to the monosodium glutamate-glucose medium in order to improve Talaromyces amestolkiae red colorant production in the incubator shaker. MSG and pH played an important role in generating stress, which affected cell growth and colorants production. The colorant synthesis seems to be is triggered under metabolic stress. Next, the effects of starter culture composition (MSG-glucose and nitrogen complex starter media) and airflow (2.0 and 8.0 Lmin−1) were investigated in the bioreactor. After 120 h of cultivation in the bioreactor, a maximum red colorant production of 9.33 UA500nm, 72.38 % glucose consumption, and 4.53 g L-1 biomass concentration were achieved under complex nitrogen as starter medium at 8 Lmin−1. Growth remained at moderate level, probably affected by the synergistic effect of MSG and pH as stress factors. Moreover, pellets formation favored suitable oxygenation of the culture broth leading to the best consumption of the carbon source. |
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Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactorBioreactorCell morphologyNatural colorantsScaling-upTalaromyces amestolkiaeFungi bioproducts have recently become commercially important to the food and pharmaceutical industry. However, different structural forms exhibited by filamentous fungi throughout their life cycles are often a bottleneck for productivity in many industrial processes. In this work, the relationship among mycelium accumulation, morphology, and colorant production was evaluated. Experiments were performed in an incubator shaker and in a stirred-tank bioreactor. First, a series of nitrogen sources and pure vitamins were investigated as additives to the monosodium glutamate-glucose medium in order to improve Talaromyces amestolkiae red colorant production in the incubator shaker. MSG and pH played an important role in generating stress, which affected cell growth and colorants production. The colorant synthesis seems to be is triggered under metabolic stress. Next, the effects of starter culture composition (MSG-glucose and nitrogen complex starter media) and airflow (2.0 and 8.0 Lmin−1) were investigated in the bioreactor. After 120 h of cultivation in the bioreactor, a maximum red colorant production of 9.33 UA500nm, 72.38 % glucose consumption, and 4.53 g L-1 biomass concentration were achieved under complex nitrogen as starter medium at 8 Lmin−1. Growth remained at moderate level, probably affected by the synergistic effect of MSG and pH as stress factors. Moreover, pellets formation favored suitable oxygenation of the culture broth leading to the best consumption of the carbon source.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista - UNESPCulture Collection DPUA/UFAM Universidade Federal do AmazonasDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista - UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal do Amazonasde Oliveira, Fernanda [UNESP]Ferreira, Laura Carmona [UNESP]Neto, Álvaro Baptista [UNESP]Simas Teixeira, Maria Franciscade Carvalho Santos Ebinuma, Valéria [UNESP]2020-12-12T01:29:36Z2020-12-12T01:29:36Z2020-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bej.2020.107694Biochemical Engineering Journal, v. 161.1873-295X1369-703Xhttp://hdl.handle.net/11449/19905810.1016/j.bej.2020.1076942-s2.0-85087315062Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical Engineering Journalinfo:eu-repo/semantics/openAccess2021-10-23T02:05:41Zoai:repositorio.unesp.br:11449/199058Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T02:05:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
title |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
spellingShingle |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor de Oliveira, Fernanda [UNESP] Bioreactor Cell morphology Natural colorants Scaling-up Talaromyces amestolkiae |
title_short |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
title_full |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
title_fullStr |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
title_full_unstemmed |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
title_sort |
Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor |
author |
de Oliveira, Fernanda [UNESP] |
author_facet |
de Oliveira, Fernanda [UNESP] Ferreira, Laura Carmona [UNESP] Neto, Álvaro Baptista [UNESP] Simas Teixeira, Maria Francisca de Carvalho Santos Ebinuma, Valéria [UNESP] |
author_role |
author |
author2 |
Ferreira, Laura Carmona [UNESP] Neto, Álvaro Baptista [UNESP] Simas Teixeira, Maria Francisca de Carvalho Santos Ebinuma, Valéria [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Amazonas |
dc.contributor.author.fl_str_mv |
de Oliveira, Fernanda [UNESP] Ferreira, Laura Carmona [UNESP] Neto, Álvaro Baptista [UNESP] Simas Teixeira, Maria Francisca de Carvalho Santos Ebinuma, Valéria [UNESP] |
dc.subject.por.fl_str_mv |
Bioreactor Cell morphology Natural colorants Scaling-up Talaromyces amestolkiae |
topic |
Bioreactor Cell morphology Natural colorants Scaling-up Talaromyces amestolkiae |
description |
Fungi bioproducts have recently become commercially important to the food and pharmaceutical industry. However, different structural forms exhibited by filamentous fungi throughout their life cycles are often a bottleneck for productivity in many industrial processes. In this work, the relationship among mycelium accumulation, morphology, and colorant production was evaluated. Experiments were performed in an incubator shaker and in a stirred-tank bioreactor. First, a series of nitrogen sources and pure vitamins were investigated as additives to the monosodium glutamate-glucose medium in order to improve Talaromyces amestolkiae red colorant production in the incubator shaker. MSG and pH played an important role in generating stress, which affected cell growth and colorants production. The colorant synthesis seems to be is triggered under metabolic stress. Next, the effects of starter culture composition (MSG-glucose and nitrogen complex starter media) and airflow (2.0 and 8.0 Lmin−1) were investigated in the bioreactor. After 120 h of cultivation in the bioreactor, a maximum red colorant production of 9.33 UA500nm, 72.38 % glucose consumption, and 4.53 g L-1 biomass concentration were achieved under complex nitrogen as starter medium at 8 Lmin−1. Growth remained at moderate level, probably affected by the synergistic effect of MSG and pH as stress factors. Moreover, pellets formation favored suitable oxygenation of the culture broth leading to the best consumption of the carbon source. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:29:36Z 2020-12-12T01:29:36Z 2020-09-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bej.2020.107694 Biochemical Engineering Journal, v. 161. 1873-295X 1369-703X http://hdl.handle.net/11449/199058 10.1016/j.bej.2020.107694 2-s2.0-85087315062 |
url |
http://dx.doi.org/10.1016/j.bej.2020.107694 http://hdl.handle.net/11449/199058 |
identifier_str_mv |
Biochemical Engineering Journal, v. 161. 1873-295X 1369-703X 10.1016/j.bej.2020.107694 2-s2.0-85087315062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical Engineering Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046542725087232 |