Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Fernanda [UNESP]
Data de Publicação: 2021
Outros Autores: Hirai, Patrícia Rie [UNESP], Teixeira, Maria Francisca Simas, Pereira, Jorge F.B. [UNESP], Santos-Ebinuma, Valéria C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.seppur.2020.117759
http://hdl.handle.net/11449/205359
Resumo: Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells.
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spelling Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquidsCell disruptionColorantsFilamentous fungiIonic liquidsTalaromyces amesolkiaeUltrasoundRecent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)Culture Collection DPUA/UFAM Universidade Federal do AmazonasUniv Coimbra CIEPQPF Department of Chemical Engineering Rua Sílvio Lima, Polo II - Pinhal de MarrocosDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)FAPESP: 2014/16424-7FAPESP: FAPESP 2014/01580-3Universidade Estadual Paulista (Unesp)Universidade Federal do AmazonasRua Sílvio Limade Oliveira, Fernanda [UNESP]Hirai, Patrícia Rie [UNESP]Teixeira, Maria Francisca SimasPereira, Jorge F.B. [UNESP]Santos-Ebinuma, Valéria C. [UNESP]2021-06-25T10:14:02Z2021-06-25T10:14:02Z2021-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.seppur.2020.117759Separation and Purification Technology, v. 257.1873-37941383-5866http://hdl.handle.net/11449/20535910.1016/j.seppur.2020.1177592-s2.0-85093649003Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSeparation and Purification Technologyinfo:eu-repo/semantics/openAccess2021-10-23T12:39:37Zoai:repositorio.unesp.br:11449/205359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:10:21.678473Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
title Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
spellingShingle Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
de Oliveira, Fernanda [UNESP]
Cell disruption
Colorants
Filamentous fungi
Ionic liquids
Talaromyces amesolkiae
Ultrasound
title_short Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
title_full Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
title_fullStr Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
title_full_unstemmed Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
title_sort Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
author de Oliveira, Fernanda [UNESP]
author_facet de Oliveira, Fernanda [UNESP]
Hirai, Patrícia Rie [UNESP]
Teixeira, Maria Francisca Simas
Pereira, Jorge F.B. [UNESP]
Santos-Ebinuma, Valéria C. [UNESP]
author_role author
author2 Hirai, Patrícia Rie [UNESP]
Teixeira, Maria Francisca Simas
Pereira, Jorge F.B. [UNESP]
Santos-Ebinuma, Valéria C. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Amazonas
Rua Sílvio Lima
dc.contributor.author.fl_str_mv de Oliveira, Fernanda [UNESP]
Hirai, Patrícia Rie [UNESP]
Teixeira, Maria Francisca Simas
Pereira, Jorge F.B. [UNESP]
Santos-Ebinuma, Valéria C. [UNESP]
dc.subject.por.fl_str_mv Cell disruption
Colorants
Filamentous fungi
Ionic liquids
Talaromyces amesolkiae
Ultrasound
topic Cell disruption
Colorants
Filamentous fungi
Ionic liquids
Talaromyces amesolkiae
Ultrasound
description Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:14:02Z
2021-06-25T10:14:02Z
2021-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.seppur.2020.117759
Separation and Purification Technology, v. 257.
1873-3794
1383-5866
http://hdl.handle.net/11449/205359
10.1016/j.seppur.2020.117759
2-s2.0-85093649003
url http://dx.doi.org/10.1016/j.seppur.2020.117759
http://hdl.handle.net/11449/205359
identifier_str_mv Separation and Purification Technology, v. 257.
1873-3794
1383-5866
10.1016/j.seppur.2020.117759
2-s2.0-85093649003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Separation and Purification Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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