Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.seppur.2020.117759 http://hdl.handle.net/11449/205359 |
Resumo: | Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells. |
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Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquidsCell disruptionColorantsFilamentous fungiIonic liquidsTalaromyces amesolkiaeUltrasoundRecent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)Culture Collection DPUA/UFAM Universidade Federal do AmazonasUniv Coimbra CIEPQPF Department of Chemical Engineering Rua Sílvio Lima, Polo II - Pinhal de MarrocosDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)FAPESP: 2014/16424-7FAPESP: FAPESP 2014/01580-3Universidade Estadual Paulista (Unesp)Universidade Federal do AmazonasRua Sílvio Limade Oliveira, Fernanda [UNESP]Hirai, Patrícia Rie [UNESP]Teixeira, Maria Francisca SimasPereira, Jorge F.B. [UNESP]Santos-Ebinuma, Valéria C. [UNESP]2021-06-25T10:14:02Z2021-06-25T10:14:02Z2021-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.seppur.2020.117759Separation and Purification Technology, v. 257.1873-37941383-5866http://hdl.handle.net/11449/20535910.1016/j.seppur.2020.1177592-s2.0-85093649003Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSeparation and Purification Technologyinfo:eu-repo/semantics/openAccess2021-10-23T12:39:37Zoai:repositorio.unesp.br:11449/205359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:10:21.678473Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
title |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
spellingShingle |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids de Oliveira, Fernanda [UNESP] Cell disruption Colorants Filamentous fungi Ionic liquids Talaromyces amesolkiae Ultrasound |
title_short |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
title_full |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
title_fullStr |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
title_full_unstemmed |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
title_sort |
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids |
author |
de Oliveira, Fernanda [UNESP] |
author_facet |
de Oliveira, Fernanda [UNESP] Hirai, Patrícia Rie [UNESP] Teixeira, Maria Francisca Simas Pereira, Jorge F.B. [UNESP] Santos-Ebinuma, Valéria C. [UNESP] |
author_role |
author |
author2 |
Hirai, Patrícia Rie [UNESP] Teixeira, Maria Francisca Simas Pereira, Jorge F.B. [UNESP] Santos-Ebinuma, Valéria C. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Amazonas Rua Sílvio Lima |
dc.contributor.author.fl_str_mv |
de Oliveira, Fernanda [UNESP] Hirai, Patrícia Rie [UNESP] Teixeira, Maria Francisca Simas Pereira, Jorge F.B. [UNESP] Santos-Ebinuma, Valéria C. [UNESP] |
dc.subject.por.fl_str_mv |
Cell disruption Colorants Filamentous fungi Ionic liquids Talaromyces amesolkiae Ultrasound |
topic |
Cell disruption Colorants Filamentous fungi Ionic liquids Talaromyces amesolkiae Ultrasound |
description |
Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([Cnmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C4mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C4mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C4mim]Cl solution, achieving a maximum recovery of 76.07 UA490nm/mL of red colorants. The results demonstrate the potential of [C4mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:14:02Z 2021-06-25T10:14:02Z 2021-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.seppur.2020.117759 Separation and Purification Technology, v. 257. 1873-3794 1383-5866 http://hdl.handle.net/11449/205359 10.1016/j.seppur.2020.117759 2-s2.0-85093649003 |
url |
http://dx.doi.org/10.1016/j.seppur.2020.117759 http://hdl.handle.net/11449/205359 |
identifier_str_mv |
Separation and Purification Technology, v. 257. 1873-3794 1383-5866 10.1016/j.seppur.2020.117759 2-s2.0-85093649003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Separation and Purification Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128225357856768 |