Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2019-0519 http://hdl.handle.net/11449/200365 |
Resumo: | Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective: To determine the antibacterial and immune modulatory activities of the POH. Methodology: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results: The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 μM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 μM (p<0.05) and 250 μM (p<0.01). The POH increased ROS production at both 10 μM and 100 μM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 μM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion: The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 μM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages. |
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Repositório Institucional da UNESP |
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Perillyl alcohol has antibacterial effects and reduces ROS production in macrophagesImmunomodulationMacrophagesNatural productsPeriodontal diseasesNatural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective: To determine the antibacterial and immune modulatory activities of the POH. Methodology: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results: The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 μM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 μM (p<0.05) and 250 μM (p<0.01). The POH increased ROS production at both 10 μM and 100 μM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 μM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion: The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 μM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal da Paraíba Programa de Pós-Graduação em OdontologiaUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e CirurgiaUniversidade de São Paulo Instituto de Ciências Biológicas Departamento de MicrobiologiaUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e CirurgiaUniversidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Figueiredo, Rebeca Dantas AlvesOrtega, Adriana Cabrera [UNESP]González Maldonado, Laura Andrea [UNESP]de Castro, Ricardo DiasÁvila-Campos, Mario JulioRossa Junior, Carlos [UNESP]de Aquino, Sabrina Garcia2020-12-12T02:04:43Z2020-12-12T02:04:43Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://dx.doi.org/10.1590/1678-7757-2019-0519Journal of Applied Oral Science, v. 28, p. 1-8.1678-77651678-7757http://hdl.handle.net/11449/20036510.1590/1678-7757-2019-0519S1678-775720200001004372-s2.0-85084170022S1678-77572020000100437.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2023-10-05T06:09:36Zoai:repositorio.unesp.br:11449/200365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:19.368442Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
title |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
spellingShingle |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages Figueiredo, Rebeca Dantas Alves Immunomodulation Macrophages Natural products Periodontal diseases |
title_short |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
title_full |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
title_fullStr |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
title_full_unstemmed |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
title_sort |
Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages |
author |
Figueiredo, Rebeca Dantas Alves |
author_facet |
Figueiredo, Rebeca Dantas Alves Ortega, Adriana Cabrera [UNESP] González Maldonado, Laura Andrea [UNESP] de Castro, Ricardo Dias Ávila-Campos, Mario Julio Rossa Junior, Carlos [UNESP] de Aquino, Sabrina Garcia |
author_role |
author |
author2 |
Ortega, Adriana Cabrera [UNESP] González Maldonado, Laura Andrea [UNESP] de Castro, Ricardo Dias Ávila-Campos, Mario Julio Rossa Junior, Carlos [UNESP] de Aquino, Sabrina Garcia |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal da Paraíba (UFPB) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Figueiredo, Rebeca Dantas Alves Ortega, Adriana Cabrera [UNESP] González Maldonado, Laura Andrea [UNESP] de Castro, Ricardo Dias Ávila-Campos, Mario Julio Rossa Junior, Carlos [UNESP] de Aquino, Sabrina Garcia |
dc.subject.por.fl_str_mv |
Immunomodulation Macrophages Natural products Periodontal diseases |
topic |
Immunomodulation Macrophages Natural products Periodontal diseases |
description |
Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective: To determine the antibacterial and immune modulatory activities of the POH. Methodology: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results: The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 μM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 μM (p<0.05) and 250 μM (p<0.01). The POH increased ROS production at both 10 μM and 100 μM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 μM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion: The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 μM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:04:43Z 2020-12-12T02:04:43Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2019-0519 Journal of Applied Oral Science, v. 28, p. 1-8. 1678-7765 1678-7757 http://hdl.handle.net/11449/200365 10.1590/1678-7757-2019-0519 S1678-77572020000100437 2-s2.0-85084170022 S1678-77572020000100437.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2019-0519 http://hdl.handle.net/11449/200365 |
identifier_str_mv |
Journal of Applied Oral Science, v. 28, p. 1-8. 1678-7765 1678-7757 10.1590/1678-7757-2019-0519 S1678-77572020000100437 2-s2.0-85084170022 S1678-77572020000100437.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128318230233088 |