Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/VETSCI6010022 http://hdl.handle.net/11449/189465 |
Resumo: | This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies. |
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Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissuesDogExtracellular matrixImmunohistochemistryPicrosiriusProstatic tissueThis study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)CNPq: 422139/2018-1CNPq: 443884/2014-5Universidade Estadual Paulista (Unesp)Calderón, Luis Gabriel Rivera [UNESP]Kobayashi, Priscila Emiko [UNESP]Vasconcelos, Rosemeri Oliveira [UNESP]Fonseca-Alves, Carlos Eduardo [UNESP]Laufer-Amorim, Renée [UNESP]2019-10-06T16:41:33Z2019-10-06T16:41:33Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/VETSCI6010022Veterinary Sciences, v. 6, n. 1, 2019.2306-7381http://hdl.handle.net/11449/18946510.3390/VETSCI60100222-s2.0-85069860992Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T15:01:05Zoai:repositorio.unesp.br:11449/189465Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:35:26.036379Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
title |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
spellingShingle |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues Calderón, Luis Gabriel Rivera [UNESP] Dog Extracellular matrix Immunohistochemistry Picrosirius Prostatic tissue |
title_short |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
title_full |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
title_fullStr |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
title_full_unstemmed |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
title_sort |
Characterization of collagen fibers (I, III, IV) and elastin of normal and neoplastic canine prostatic tissues |
author |
Calderón, Luis Gabriel Rivera [UNESP] |
author_facet |
Calderón, Luis Gabriel Rivera [UNESP] Kobayashi, Priscila Emiko [UNESP] Vasconcelos, Rosemeri Oliveira [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Laufer-Amorim, Renée [UNESP] |
author_role |
author |
author2 |
Kobayashi, Priscila Emiko [UNESP] Vasconcelos, Rosemeri Oliveira [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Laufer-Amorim, Renée [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Calderón, Luis Gabriel Rivera [UNESP] Kobayashi, Priscila Emiko [UNESP] Vasconcelos, Rosemeri Oliveira [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Laufer-Amorim, Renée [UNESP] |
dc.subject.por.fl_str_mv |
Dog Extracellular matrix Immunohistochemistry Picrosirius Prostatic tissue |
topic |
Dog Extracellular matrix Immunohistochemistry Picrosirius Prostatic tissue |
description |
This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:41:33Z 2019-10-06T16:41:33Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/VETSCI6010022 Veterinary Sciences, v. 6, n. 1, 2019. 2306-7381 http://hdl.handle.net/11449/189465 10.3390/VETSCI6010022 2-s2.0-85069860992 |
url |
http://dx.doi.org/10.3390/VETSCI6010022 http://hdl.handle.net/11449/189465 |
identifier_str_mv |
Veterinary Sciences, v. 6, n. 1, 2019. 2306-7381 10.3390/VETSCI6010022 2-s2.0-85069860992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129091838148608 |