Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate

Detalhes bibliográficos
Autor(a) principal: Fonseca-Alves, Carlos Eduardo [UNESP]
Data de Publicação: 2017
Outros Autores: Kobayashi, Priscilla Emiko [UNESP], Palmieri, Chiara, Laufer-Amorim, Renée [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12917-017-1304-0
http://hdl.handle.net/11449/175604
Resumo: Background: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer.
id UNSP_52dfb09c4600fab1521c295e23a6bbc2
oai_identifier_str oai:repositorio.unesp.br:11449/175604
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostateCD117 antigenDogImmunohistochemistryProstatic cancerWestern blottingBackground: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer.Univ. Estadual Paulista - UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science, Rua Prof. Dr Walter Correa, s/n, Unesp, Mail box- 560The University of Queensland Gatton Campus School of Veterinary ScienceUniv. Estadual Paulista - UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science, Rua Prof. Dr Walter Correa, s/n, Unesp, Mail box- 560Universidade Estadual Paulista (Unesp)School of Veterinary ScienceFonseca-Alves, Carlos Eduardo [UNESP]Kobayashi, Priscilla Emiko [UNESP]Palmieri, ChiaraLaufer-Amorim, Renée [UNESP]2018-12-11T17:16:40Z2018-12-11T17:16:40Z2017-12-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12917-017-1304-0BMC Veterinary Research, v. 13, n. 1, 2017.1746-6148http://hdl.handle.net/11449/17560410.1186/s12917-017-1304-02-s2.0-850373435652-s2.0-85037343565.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research0,934info:eu-repo/semantics/openAccess2024-01-11T06:26:44Zoai:repositorio.unesp.br:11449/175604Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:23.743595Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
title Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
spellingShingle Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
Fonseca-Alves, Carlos Eduardo [UNESP]
CD117 antigen
Dog
Immunohistochemistry
Prostatic cancer
Western blotting
title_short Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
title_full Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
title_fullStr Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
title_full_unstemmed Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
title_sort Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
author Fonseca-Alves, Carlos Eduardo [UNESP]
author_facet Fonseca-Alves, Carlos Eduardo [UNESP]
Kobayashi, Priscilla Emiko [UNESP]
Palmieri, Chiara
Laufer-Amorim, Renée [UNESP]
author_role author
author2 Kobayashi, Priscilla Emiko [UNESP]
Palmieri, Chiara
Laufer-Amorim, Renée [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
School of Veterinary Science
dc.contributor.author.fl_str_mv Fonseca-Alves, Carlos Eduardo [UNESP]
Kobayashi, Priscilla Emiko [UNESP]
Palmieri, Chiara
Laufer-Amorim, Renée [UNESP]
dc.subject.por.fl_str_mv CD117 antigen
Dog
Immunohistochemistry
Prostatic cancer
Western blotting
topic CD117 antigen
Dog
Immunohistochemistry
Prostatic cancer
Western blotting
description Background: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-06
2018-12-11T17:16:40Z
2018-12-11T17:16:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12917-017-1304-0
BMC Veterinary Research, v. 13, n. 1, 2017.
1746-6148
http://hdl.handle.net/11449/175604
10.1186/s12917-017-1304-0
2-s2.0-85037343565
2-s2.0-85037343565.pdf
url http://dx.doi.org/10.1186/s12917-017-1304-0
http://hdl.handle.net/11449/175604
identifier_str_mv BMC Veterinary Research, v. 13, n. 1, 2017.
1746-6148
10.1186/s12917-017-1304-0
2-s2.0-85037343565
2-s2.0-85037343565.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Veterinary Research
0,934
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129449949921280