Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12917-017-1304-0 http://hdl.handle.net/11449/175604 |
Resumo: | Background: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer. |
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Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostateCD117 antigenDogImmunohistochemistryProstatic cancerWestern blottingBackground: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer.Univ. Estadual Paulista - UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science, Rua Prof. Dr Walter Correa, s/n, Unesp, Mail box- 560The University of Queensland Gatton Campus School of Veterinary ScienceUniv. Estadual Paulista - UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science, Rua Prof. Dr Walter Correa, s/n, Unesp, Mail box- 560Universidade Estadual Paulista (Unesp)School of Veterinary ScienceFonseca-Alves, Carlos Eduardo [UNESP]Kobayashi, Priscilla Emiko [UNESP]Palmieri, ChiaraLaufer-Amorim, Renée [UNESP]2018-12-11T17:16:40Z2018-12-11T17:16:40Z2017-12-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12917-017-1304-0BMC Veterinary Research, v. 13, n. 1, 2017.1746-6148http://hdl.handle.net/11449/17560410.1186/s12917-017-1304-02-s2.0-850373435652-s2.0-85037343565.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research0,934info:eu-repo/semantics/openAccess2024-01-11T06:26:44Zoai:repositorio.unesp.br:11449/175604Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:23.743595Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
title |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
spellingShingle |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate Fonseca-Alves, Carlos Eduardo [UNESP] CD117 antigen Dog Immunohistochemistry Prostatic cancer Western blotting |
title_short |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
title_full |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
title_fullStr |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
title_full_unstemmed |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
title_sort |
Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate |
author |
Fonseca-Alves, Carlos Eduardo [UNESP] |
author_facet |
Fonseca-Alves, Carlos Eduardo [UNESP] Kobayashi, Priscilla Emiko [UNESP] Palmieri, Chiara Laufer-Amorim, Renée [UNESP] |
author_role |
author |
author2 |
Kobayashi, Priscilla Emiko [UNESP] Palmieri, Chiara Laufer-Amorim, Renée [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) School of Veterinary Science |
dc.contributor.author.fl_str_mv |
Fonseca-Alves, Carlos Eduardo [UNESP] Kobayashi, Priscilla Emiko [UNESP] Palmieri, Chiara Laufer-Amorim, Renée [UNESP] |
dc.subject.por.fl_str_mv |
CD117 antigen Dog Immunohistochemistry Prostatic cancer Western blotting |
topic |
CD117 antigen Dog Immunohistochemistry Prostatic cancer Western blotting |
description |
Background: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-06 2018-12-11T17:16:40Z 2018-12-11T17:16:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12917-017-1304-0 BMC Veterinary Research, v. 13, n. 1, 2017. 1746-6148 http://hdl.handle.net/11449/175604 10.1186/s12917-017-1304-0 2-s2.0-85037343565 2-s2.0-85037343565.pdf |
url |
http://dx.doi.org/10.1186/s12917-017-1304-0 http://hdl.handle.net/11449/175604 |
identifier_str_mv |
BMC Veterinary Research, v. 13, n. 1, 2017. 1746-6148 10.1186/s12917-017-1304-0 2-s2.0-85037343565 2-s2.0-85037343565.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Veterinary Research 0,934 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129449949921280 |