In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I

Detalhes bibliográficos
Autor(a) principal: Faria, Karina de Cassia [UNESP]
Data de Publicação: 2002
Outros Autores: Morielle-Versute, Eliana [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572002000400003
http://hdl.handle.net/11449/21466
Resumo: The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition.
id UNSP_170802051313eaa170b36783869536b5
oai_identifier_str oai:repositorio.unesp.br:11449/21466
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu IAluIFishChiropteratelomeric sitesDAPIThe purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP IBILCE Departamento de BiologiaUNESP IBILCE Departamento de Zoologia e BotânicaUNESP IBILCE Departamento de BiologiaUNESP IBILCE Departamento de Zoologia e BotânicaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Faria, Karina de Cassia [UNESP]Morielle-Versute, Eliana [UNESP]2014-05-20T14:00:46Z2014-05-20T14:00:46Z2002-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-371application/pdfhttp://dx.doi.org/10.1590/S1415-47572002000400003Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002.1415-4757http://hdl.handle.net/11449/2146610.1590/S1415-47572002000400003S1415-47572002000400003S1415-47572002000400003.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-23T06:15:18Zoai:repositorio.unesp.br:11449/21466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:03:54.751702Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
title In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
spellingShingle In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
Faria, Karina de Cassia [UNESP]
AluI
Fish
Chiroptera
telomeric sites
DAPI
title_short In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
title_full In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
title_fullStr In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
title_full_unstemmed In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
title_sort In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
author Faria, Karina de Cassia [UNESP]
author_facet Faria, Karina de Cassia [UNESP]
Morielle-Versute, Eliana [UNESP]
author_role author
author2 Morielle-Versute, Eliana [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Faria, Karina de Cassia [UNESP]
Morielle-Versute, Eliana [UNESP]
dc.subject.por.fl_str_mv AluI
Fish
Chiroptera
telomeric sites
DAPI
topic AluI
Fish
Chiroptera
telomeric sites
DAPI
description The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
2014-05-20T14:00:46Z
2014-05-20T14:00:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572002000400003
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002.
1415-4757
http://hdl.handle.net/11449/21466
10.1590/S1415-47572002000400003
S1415-47572002000400003
S1415-47572002000400003.pdf
url http://dx.doi.org/10.1590/S1415-47572002000400003
http://hdl.handle.net/11449/21466
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002.
1415-4757
10.1590/S1415-47572002000400003
S1415-47572002000400003
S1415-47572002000400003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 365-371
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129280152961024