In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572002000400003 http://hdl.handle.net/11449/21466 |
Resumo: | The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition. |
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Repositório Institucional da UNESP |
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spelling |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu IAluIFishChiropteratelomeric sitesDAPIThe purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP IBILCE Departamento de BiologiaUNESP IBILCE Departamento de Zoologia e BotânicaUNESP IBILCE Departamento de BiologiaUNESP IBILCE Departamento de Zoologia e BotânicaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Faria, Karina de Cassia [UNESP]Morielle-Versute, Eliana [UNESP]2014-05-20T14:00:46Z2014-05-20T14:00:46Z2002-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-371application/pdfhttp://dx.doi.org/10.1590/S1415-47572002000400003Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002.1415-4757http://hdl.handle.net/11449/2146610.1590/S1415-47572002000400003S1415-47572002000400003S1415-47572002000400003.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-23T06:15:18Zoai:repositorio.unesp.br:11449/21466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:03:54.751702Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
title |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
spellingShingle |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I Faria, Karina de Cassia [UNESP] AluI Fish Chiroptera telomeric sites DAPI |
title_short |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
title_full |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
title_fullStr |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
title_full_unstemmed |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
title_sort |
In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I |
author |
Faria, Karina de Cassia [UNESP] |
author_facet |
Faria, Karina de Cassia [UNESP] Morielle-Versute, Eliana [UNESP] |
author_role |
author |
author2 |
Morielle-Versute, Eliana [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Faria, Karina de Cassia [UNESP] Morielle-Versute, Eliana [UNESP] |
dc.subject.por.fl_str_mv |
AluI Fish Chiroptera telomeric sites DAPI |
topic |
AluI Fish Chiroptera telomeric sites DAPI |
description |
The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band- positive regions of bat chromosomes have a different DNA composition. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-01-01 2014-05-20T14:00:46Z 2014-05-20T14:00:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572002000400003 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002. 1415-4757 http://hdl.handle.net/11449/21466 10.1590/S1415-47572002000400003 S1415-47572002000400003 S1415-47572002000400003.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572002000400003 http://hdl.handle.net/11449/21466 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 4, p. 365-371, 2002. 1415-4757 10.1590/S1415-47572002000400003 S1415-47572002000400003 S1415-47572002000400003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
365-371 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129280152961024 |