Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth

Detalhes bibliográficos
Autor(a) principal: Ximenes, Rafael M.
Data de Publicação: 2012
Outros Autores: Rabello, Marcelo M., Araujo, Renata M., Silveira, Edilberto R., Fagundes, Fabio H. R. [UNESP], Diz-Filho, Eduardo B. S. [UNESP], Buzzo, Simone C. [UNESP], Soares, Veronica C. G. [UNESP], Toyama, Daniela de O., Gaeta, Henrique H. [UNESP], Hernandes, Marcelo Z., Monteiro, Helena S. A., Toyama, Marcos H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2012/987517
http://hdl.handle.net/11449/381
Resumo: Secretory phospholipases A(2) (sPLA(2)) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated from Harpalyce brasiliana Benth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2 from Bothrops pirajai, Crotalus durissus terrificus, Apis mellifera, and Naja naja venoms. Har2 inhibits all sPLA(2) tested. PrTX-III (B. pirajai venom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificus venom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. naja venom) at 88.1%. Edema induced by exogenous sPLA(2) administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA(2)s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA(2) inhibition.
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spelling Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana BenthSecretory phospholipases A(2) (sPLA(2)) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated from Harpalyce brasiliana Benth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2 from Bothrops pirajai, Crotalus durissus terrificus, Apis mellifera, and Naja naja venoms. Har2 inhibits all sPLA(2) tested. PrTX-III (B. pirajai venom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificus venom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. naja venom) at 88.1%. Edema induced by exogenous sPLA(2) administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA(2)s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA(2) inhibition.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP)Univ Fed Ceara, Dept Fisiol & Farmacol, BR-60430270 Fortaleza, CE, BrazilUniversidade Federal de Pernambuco (UFPE), Dept Ciencias Farmaceut, BR-50740520 Recife, PE, BrazilUniv Fed Rio Grande do Norte, Dept Quim, BR-50078970 Natal, RN, BrazilUniv Fed Ceara, Ctr NE Aplicacao & Uso Ressonancia Magnet Nucl CE, BR-60455760 Fortaleza, CE, BrazilUniv Estadual Campinas, Inst Biomed, Dept Bioquim, BR-13082862 Campinas, SP, BrazilUniv Estadual Paulista, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP, BrazilUniv Presbiteriana Mackenzie, Ctr Ciencias Biol & Saude, BR-01302970 São Paulo, BrazilUniv Estadual Paulista, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP, BrazilHindawi Publishing CorporationUniversidade Federal do Ceará (UFC)Universidade Federal de Pernambuco (UFPE)Universidade Federal do Rio Grande do Norte (UFRN)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Univ Presbiteriana MackenzieXimenes, Rafael M.Rabello, Marcelo M.Araujo, Renata M.Silveira, Edilberto R.Fagundes, Fabio H. R. [UNESP]Diz-Filho, Eduardo B. S. [UNESP]Buzzo, Simone C. [UNESP]Soares, Veronica C. G. [UNESP]Toyama, Daniela de O.Gaeta, Henrique H. [UNESP]Hernandes, Marcelo Z.Monteiro, Helena S. A.Toyama, Marcos H. [UNESP]2014-05-20T13:12:24Z2014-05-20T13:12:24Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1155/2012/987517Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 9, 2012.1741-427Xhttp://hdl.handle.net/11449/38110.1155/2012/987517WOS:000308300500001WOS000308300500001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccess2023-12-08T06:23:18Zoai:repositorio.unesp.br:11449/381Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:48:17.589352Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
title Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
spellingShingle Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
Ximenes, Rafael M.
title_short Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
title_full Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
title_fullStr Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
title_full_unstemmed Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
title_sort Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
author Ximenes, Rafael M.
author_facet Ximenes, Rafael M.
Rabello, Marcelo M.
Araujo, Renata M.
Silveira, Edilberto R.
Fagundes, Fabio H. R. [UNESP]
Diz-Filho, Eduardo B. S. [UNESP]
Buzzo, Simone C. [UNESP]
Soares, Veronica C. G. [UNESP]
Toyama, Daniela de O.
Gaeta, Henrique H. [UNESP]
Hernandes, Marcelo Z.
Monteiro, Helena S. A.
Toyama, Marcos H. [UNESP]
author_role author
author2 Rabello, Marcelo M.
Araujo, Renata M.
Silveira, Edilberto R.
Fagundes, Fabio H. R. [UNESP]
Diz-Filho, Eduardo B. S. [UNESP]
Buzzo, Simone C. [UNESP]
Soares, Veronica C. G. [UNESP]
Toyama, Daniela de O.
Gaeta, Henrique H. [UNESP]
Hernandes, Marcelo Z.
Monteiro, Helena S. A.
Toyama, Marcos H. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Ceará (UFC)
Universidade Federal de Pernambuco (UFPE)
Universidade Federal do Rio Grande do Norte (UFRN)
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
Univ Presbiteriana Mackenzie
dc.contributor.author.fl_str_mv Ximenes, Rafael M.
Rabello, Marcelo M.
Araujo, Renata M.
Silveira, Edilberto R.
Fagundes, Fabio H. R. [UNESP]
Diz-Filho, Eduardo B. S. [UNESP]
Buzzo, Simone C. [UNESP]
Soares, Veronica C. G. [UNESP]
Toyama, Daniela de O.
Gaeta, Henrique H. [UNESP]
Hernandes, Marcelo Z.
Monteiro, Helena S. A.
Toyama, Marcos H. [UNESP]
description Secretory phospholipases A(2) (sPLA(2)) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated from Harpalyce brasiliana Benth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2 from Bothrops pirajai, Crotalus durissus terrificus, Apis mellifera, and Naja naja venoms. Har2 inhibits all sPLA(2) tested. PrTX-III (B. pirajai venom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificus venom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. naja venom) at 88.1%. Edema induced by exogenous sPLA(2) administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA(2)s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA(2) inhibition.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2014-05-20T13:12:24Z
2014-05-20T13:12:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2012/987517
Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 9, 2012.
1741-427X
http://hdl.handle.net/11449/381
10.1155/2012/987517
WOS:000308300500001
WOS000308300500001.pdf
url http://dx.doi.org/10.1155/2012/987517
http://hdl.handle.net/11449/381
identifier_str_mv Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 9, 2012.
1741-427X
10.1155/2012/987517
WOS:000308300500001
WOS000308300500001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-based Complementary and Alternative Medicine
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application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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