Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms22168669 http://hdl.handle.net/11449/233548 |
Resumo: | Prostate cancer (PCa) is the leading cause of cancer-associated mortality in men, and new biomarkers are still needed. The expression pattern and protein tissue localization of proteoglycans of the syndecan family (SDC 1-4) and syntenin-1 (SDCBP) were determined in normal and prostatic tumor tissue from two genetically engineered mouse models and human prostate tumors. Studies were validated using SDC 1-4 and SDCBP mRNA levels and patient survival data from The Cancer Genome Atlas and CamCAP databases. RNAseq showed increased expression of Sdc1 in Pb-Cre4/Ptenf/f mouse Pca and upregulation of Sdc3 expression and downregulation of Sdc2 and Sdc4 when compared to the normal prostatic tissue in Pb-Cre4/Trp53f/f-;Rb1f/f mouse tumors. These changes were confirmed by immunohistochemistry. In human PCa, SDC 1-4 and SDCBP immunostaining showed variable localization. Furthermore, Kaplan-Meier analysis showed that patients expressing SDC3 had shorter prostate-specific survival than those without SDC3 expression (log-rank test, p = 0.0047). Analysis of the MSKCC-derived expression showed that SDC1 and SDC3 overexpression is predictive of decreased biochemical recurrence-free survival (p = 0.0099 and p = 0.045, respectively), and SDC4 overexpression is predictive of increased biochemical recurrence-free survival (p = 0.035). SDC4 overexpression was associated with a better prognosis, while SDC1 and SDC3 were associated with more aggressive tumors and a worse prognosis. |
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Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancergene expressionoutcomeprognostic markerprostate cancersurvivalsyndecanProstate cancer (PCa) is the leading cause of cancer-associated mortality in men, and new biomarkers are still needed. The expression pattern and protein tissue localization of proteoglycans of the syndecan family (SDC 1-4) and syntenin-1 (SDCBP) were determined in normal and prostatic tumor tissue from two genetically engineered mouse models and human prostate tumors. Studies were validated using SDC 1-4 and SDCBP mRNA levels and patient survival data from The Cancer Genome Atlas and CamCAP databases. RNAseq showed increased expression of Sdc1 in Pb-Cre4/Ptenf/f mouse Pca and upregulation of Sdc3 expression and downregulation of Sdc2 and Sdc4 when compared to the normal prostatic tissue in Pb-Cre4/Trp53f/f-;Rb1f/f mouse tumors. These changes were confirmed by immunohistochemistry. In human PCa, SDC 1-4 and SDCBP immunostaining showed variable localization. Furthermore, Kaplan-Meier analysis showed that patients expressing SDC3 had shorter prostate-specific survival than those without SDC3 expression (log-rank test, p = 0.0047). Analysis of the MSKCC-derived expression showed that SDC1 and SDC3 overexpression is predictive of decreased biochemical recurrence-free survival (p = 0.0099 and p = 0.045, respectively), and SDC4 overexpression is predictive of increased biochemical recurrence-free survival (p = 0.035). SDC4 overexpression was associated with a better prognosis, while SDC1 and SDC3 were associated with more aggressive tumors and a worse prognosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional BIology Institute of Bioscience of Botucatu (IBB) São Paulo State UniversityDepartment of Structural and Functional Biology Institute of Biology (IB) UNICAMP-State University of CampinasBotucatu School of Medicine (FMB) São Paulo State UniversityDepartment of Structural and Functional BIology Institute of Bioscience of Botucatu (IBB) São Paulo State UniversityBotucatu School of Medicine (FMB) São Paulo State UniversityFAPESP: 2015/26175-7, 2016/09532-3, 2019/19644-1Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Santos, Nilton José [UNESP]Barquilha, Caroline Nascimento [UNESP]Barbosa, Isabela Correa [UNESP]Macedo, Rodrigo Tavares [UNESP]Lima, Flávio Oliveira [UNESP]Justulin, Luis Antônio [UNESP]Barbosa, Guilherme OliveiraCarvalho, Hernandes F.Felisbino, Sérgio Luis [UNESP]2022-05-01T09:30:32Z2022-05-01T09:30:32Z2021-08-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms22168669International journal of molecular sciences, v. 22, n. 16, 2021.1422-0067http://hdl.handle.net/11449/23354810.3390/ijms221686692-s2.0-85115042413Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational journal of molecular sciencesinfo:eu-repo/semantics/openAccess2022-05-01T09:30:32Zoai:repositorio.unesp.br:11449/233548Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:26:52.549371Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
title |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
spellingShingle |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer Santos, Nilton José [UNESP] gene expression outcome prognostic marker prostate cancer survival syndecan |
title_short |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
title_full |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
title_fullStr |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
title_full_unstemmed |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
title_sort |
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer |
author |
Santos, Nilton José [UNESP] |
author_facet |
Santos, Nilton José [UNESP] Barquilha, Caroline Nascimento [UNESP] Barbosa, Isabela Correa [UNESP] Macedo, Rodrigo Tavares [UNESP] Lima, Flávio Oliveira [UNESP] Justulin, Luis Antônio [UNESP] Barbosa, Guilherme Oliveira Carvalho, Hernandes F. Felisbino, Sérgio Luis [UNESP] |
author_role |
author |
author2 |
Barquilha, Caroline Nascimento [UNESP] Barbosa, Isabela Correa [UNESP] Macedo, Rodrigo Tavares [UNESP] Lima, Flávio Oliveira [UNESP] Justulin, Luis Antônio [UNESP] Barbosa, Guilherme Oliveira Carvalho, Hernandes F. Felisbino, Sérgio Luis [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Santos, Nilton José [UNESP] Barquilha, Caroline Nascimento [UNESP] Barbosa, Isabela Correa [UNESP] Macedo, Rodrigo Tavares [UNESP] Lima, Flávio Oliveira [UNESP] Justulin, Luis Antônio [UNESP] Barbosa, Guilherme Oliveira Carvalho, Hernandes F. Felisbino, Sérgio Luis [UNESP] |
dc.subject.por.fl_str_mv |
gene expression outcome prognostic marker prostate cancer survival syndecan |
topic |
gene expression outcome prognostic marker prostate cancer survival syndecan |
description |
Prostate cancer (PCa) is the leading cause of cancer-associated mortality in men, and new biomarkers are still needed. The expression pattern and protein tissue localization of proteoglycans of the syndecan family (SDC 1-4) and syntenin-1 (SDCBP) were determined in normal and prostatic tumor tissue from two genetically engineered mouse models and human prostate tumors. Studies were validated using SDC 1-4 and SDCBP mRNA levels and patient survival data from The Cancer Genome Atlas and CamCAP databases. RNAseq showed increased expression of Sdc1 in Pb-Cre4/Ptenf/f mouse Pca and upregulation of Sdc3 expression and downregulation of Sdc2 and Sdc4 when compared to the normal prostatic tissue in Pb-Cre4/Trp53f/f-;Rb1f/f mouse tumors. These changes were confirmed by immunohistochemistry. In human PCa, SDC 1-4 and SDCBP immunostaining showed variable localization. Furthermore, Kaplan-Meier analysis showed that patients expressing SDC3 had shorter prostate-specific survival than those without SDC3 expression (log-rank test, p = 0.0047). Analysis of the MSKCC-derived expression showed that SDC1 and SDC3 overexpression is predictive of decreased biochemical recurrence-free survival (p = 0.0099 and p = 0.045, respectively), and SDC4 overexpression is predictive of increased biochemical recurrence-free survival (p = 0.035). SDC4 overexpression was associated with a better prognosis, while SDC1 and SDC3 were associated with more aggressive tumors and a worse prognosis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-12 2022-05-01T09:30:32Z 2022-05-01T09:30:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms22168669 International journal of molecular sciences, v. 22, n. 16, 2021. 1422-0067 http://hdl.handle.net/11449/233548 10.3390/ijms22168669 2-s2.0-85115042413 |
url |
http://dx.doi.org/10.3390/ijms22168669 http://hdl.handle.net/11449/233548 |
identifier_str_mv |
International journal of molecular sciences, v. 22, n. 16, 2021. 1422-0067 10.3390/ijms22168669 2-s2.0-85115042413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International journal of molecular sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129522007015424 |