Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fishres.2016.06.009 http://hdl.handle.net/11449/161880 |
Resumo: | It is most difficult to identify the coexistent commercial pink shrimps Farfantepenaeus brasiliensis and Farfantepenaeus paulensis during the juvenile stage, when secondary sexual characteristits are still in the developing period. Differences in width and/or shape of the dorsal furrow on pleonite six has traditionally been the primary character used to discriminate juveniles of these two taxa, but is unreliable. This study aimed to test the hypothesis that the taxonomic morphological characteristics traditionally used to discriminate between these species are not effective for juveniles. Molecular analyses showed that the COI gene is an efficient marker for separating these two species and that traditional characteristics do not allow for the correct identification of juveniles of these species. In addition, we found that only 64% of juveniles identified a priori based on traditional morphological traits and subsequently verified molecularly were identified correctly. After molecular identification of the species, we searched for new morphological traits that could be used for reliable identification of juvenile and adult stages using morphometry and comparative morphology. The carapace length (CL) ranged from 12.5 to 26.9 mm in F. brasiliensis and from 9.4 to 49.5 mm in F. paulensis. We affirmed the efficiency of COI as a molecular marker and identified a new morphological trait that will. aid in the discrimination of juveniles of F. brasiliensis and F. paulensis. Contrary to our expectations, the characters identified by morphometric analysis were subtle and difficult to apply in field identification situations. However, when analyzing the external morphology of juveniles, it was possible to identify differences between the species in the anterior margin of gastrofrontal carina in relation to the rostrum teeth. In addition to corroborating the difficulty in identifying these two species, our study confirms the importance of the association between molecular and comparative morphology analyses in a fisheries and biodiversity context. Furthermore, we extended the geographic distribution of Farfantepenaeus subtilis through a new record from southern Cananeia, on the southern coast of Sao Paulo State. (C) 2016 Elsevier B.V. All rights reserved. |
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Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcodingBrazilFarfantepenaeusCytochrome oxidase IMorphometryPenaeoideaSpecies discriminationIt is most difficult to identify the coexistent commercial pink shrimps Farfantepenaeus brasiliensis and Farfantepenaeus paulensis during the juvenile stage, when secondary sexual characteristits are still in the developing period. Differences in width and/or shape of the dorsal furrow on pleonite six has traditionally been the primary character used to discriminate juveniles of these two taxa, but is unreliable. This study aimed to test the hypothesis that the taxonomic morphological characteristics traditionally used to discriminate between these species are not effective for juveniles. Molecular analyses showed that the COI gene is an efficient marker for separating these two species and that traditional characteristics do not allow for the correct identification of juveniles of these species. In addition, we found that only 64% of juveniles identified a priori based on traditional morphological traits and subsequently verified molecularly were identified correctly. After molecular identification of the species, we searched for new morphological traits that could be used for reliable identification of juvenile and adult stages using morphometry and comparative morphology. The carapace length (CL) ranged from 12.5 to 26.9 mm in F. brasiliensis and from 9.4 to 49.5 mm in F. paulensis. We affirmed the efficiency of COI as a molecular marker and identified a new morphological trait that will. aid in the discrimination of juveniles of F. brasiliensis and F. paulensis. Contrary to our expectations, the characters identified by morphometric analysis were subtle and difficult to apply in field identification situations. However, when analyzing the external morphology of juveniles, it was possible to identify differences between the species in the anterior margin of gastrofrontal carina in relation to the rostrum teeth. In addition to corroborating the difficulty in identifying these two species, our study confirms the importance of the association between molecular and comparative morphology analyses in a fisheries and biodiversity context. Furthermore, we extended the geographic distribution of Farfantepenaeus subtilis through a new record from southern Cananeia, on the southern coast of Sao Paulo State. (C) 2016 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista UNESP, Fac Ciencias, Dept Ciencias Biol, Lab Biol Camaroes Marinhos & Dulcicolas LABCAM, Av Engn Luiz Edmundo Corrijo Coube 14-01, BR-17033360 Bauru, SP, BrazilUniv Sao Paulo, FFCLRP, LBSC, BR-14040901 Sao Paulo, BrazilUniv Estadual Paulista UNESP, Fac Ciencias, Dept Ciencias Biol, Lab Biol Camaroes Marinhos & Dulcicolas LABCAM, Av Engn Luiz Edmundo Corrijo Coube 14-01, BR-17033360 Bauru, SP, BrazilFAPESP: Tematico Biota 2010/50188-8FAPESP: 2011/16268-7FAPESP: PD 2011/11901-3CAPES: 2005/2014 - 23038.004308/2014-14CNPq: 305919/2014-8CNPq: 304968/2014-5Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Teodoro, S. S. A. [UNESP]Terossi, M.Mantelatto, F. L.Costa, R. C. [UNESP]2018-11-26T17:04:30Z2018-11-26T17:04:30Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article244-253application/pdfhttp://dx.doi.org/10.1016/j.fishres.2016.06.009Fisheries Research. Amsterdam: Elsevier Science Bv, v. 183, p. 244-253, 2016.0165-7836http://hdl.handle.net/11449/16188010.1016/j.fishres.2016.06.009WOS:000382599600025WOS000382599600025.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFisheries Research0,941info:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/161880Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:21:30.562531Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
title |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
spellingShingle |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding Teodoro, S. S. A. [UNESP] Brazil Farfantepenaeus Cytochrome oxidase I Morphometry Penaeoidea Species discrimination |
title_short |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
title_full |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
title_fullStr |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
title_full_unstemmed |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
title_sort |
Discordance in the identification of juvenile pink shrimp (Farfantepenaeus brasiliensis and F. paulensis: Family Penaeidae): An integrative approach using morphology, morphometry and barcoding |
author |
Teodoro, S. S. A. [UNESP] |
author_facet |
Teodoro, S. S. A. [UNESP] Terossi, M. Mantelatto, F. L. Costa, R. C. [UNESP] |
author_role |
author |
author2 |
Terossi, M. Mantelatto, F. L. Costa, R. C. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Teodoro, S. S. A. [UNESP] Terossi, M. Mantelatto, F. L. Costa, R. C. [UNESP] |
dc.subject.por.fl_str_mv |
Brazil Farfantepenaeus Cytochrome oxidase I Morphometry Penaeoidea Species discrimination |
topic |
Brazil Farfantepenaeus Cytochrome oxidase I Morphometry Penaeoidea Species discrimination |
description |
It is most difficult to identify the coexistent commercial pink shrimps Farfantepenaeus brasiliensis and Farfantepenaeus paulensis during the juvenile stage, when secondary sexual characteristits are still in the developing period. Differences in width and/or shape of the dorsal furrow on pleonite six has traditionally been the primary character used to discriminate juveniles of these two taxa, but is unreliable. This study aimed to test the hypothesis that the taxonomic morphological characteristics traditionally used to discriminate between these species are not effective for juveniles. Molecular analyses showed that the COI gene is an efficient marker for separating these two species and that traditional characteristics do not allow for the correct identification of juveniles of these species. In addition, we found that only 64% of juveniles identified a priori based on traditional morphological traits and subsequently verified molecularly were identified correctly. After molecular identification of the species, we searched for new morphological traits that could be used for reliable identification of juvenile and adult stages using morphometry and comparative morphology. The carapace length (CL) ranged from 12.5 to 26.9 mm in F. brasiliensis and from 9.4 to 49.5 mm in F. paulensis. We affirmed the efficiency of COI as a molecular marker and identified a new morphological trait that will. aid in the discrimination of juveniles of F. brasiliensis and F. paulensis. Contrary to our expectations, the characters identified by morphometric analysis were subtle and difficult to apply in field identification situations. However, when analyzing the external morphology of juveniles, it was possible to identify differences between the species in the anterior margin of gastrofrontal carina in relation to the rostrum teeth. In addition to corroborating the difficulty in identifying these two species, our study confirms the importance of the association between molecular and comparative morphology analyses in a fisheries and biodiversity context. Furthermore, we extended the geographic distribution of Farfantepenaeus subtilis through a new record from southern Cananeia, on the southern coast of Sao Paulo State. (C) 2016 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T17:04:30Z 2018-11-26T17:04:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fishres.2016.06.009 Fisheries Research. Amsterdam: Elsevier Science Bv, v. 183, p. 244-253, 2016. 0165-7836 http://hdl.handle.net/11449/161880 10.1016/j.fishres.2016.06.009 WOS:000382599600025 WOS000382599600025.pdf |
url |
http://dx.doi.org/10.1016/j.fishres.2016.06.009 http://hdl.handle.net/11449/161880 |
identifier_str_mv |
Fisheries Research. Amsterdam: Elsevier Science Bv, v. 183, p. 244-253, 2016. 0165-7836 10.1016/j.fishres.2016.06.009 WOS:000382599600025 WOS000382599600025.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fisheries Research 0,941 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
244-253 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129312732217344 |