Susceptibility of mammalian cell line for isolation of IBDV from clinical samples
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2004000100009 http://hdl.handle.net/11449/14772 |
Resumo: | Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect (CPE) caused by IBDV infection was pronounced at 18h for Vero and 36h for CER cells. The RT-PCR was done to detect IBDV RNA from the bursa samples, where all were positive. Regarding to IBDV isolation, 20% of samples tested were negative in Vero cells, where all were positive in CER cells infection. The findings allow concluding that CER cell can be a new and appropriated tool for IBDV isolation. |
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Susceptibility of mammalian cell line for isolation of IBDV from clinical samplesAvibirnavirusCER cellsdiagnosisdouble antibody sandwich ELISART/PCRSusceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect (CPE) caused by IBDV infection was pronounced at 18h for Vero and 36h for CER cells. The RT-PCR was done to detect IBDV RNA from the bursa samples, where all were positive. Regarding to IBDV isolation, 20% of samples tested were negative in Vero cells, where all were positive in CER cells infection. The findings allow concluding that CER cell can be a new and appropriated tool for IBDV isolation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Odontologia Depto de Apoio, Produção e Saúde AnimalFundação de Amparo à Pesquisa do Estado de São PauloUniversidade Estadual Paulista Faculdade de Odontologia Depto de Apoio, Produção e Saúde AnimalFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Silva, S. E. L. [UNESP]Ferreira, H. L. [UNESP]Carvalho, B. A. [UNESP]Cardoso, T. C. [UNESP]2013-09-30T20:13:06Z2014-05-20T13:42:29Z2013-09-30T20:13:06Z2014-05-20T13:42:29Z2004-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article65-69application/pdfhttp://dx.doi.org/10.1590/S1516-635X2004000100009Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 1, p. 65-69, 2004.1516-635Xhttp://hdl.handle.net/11449/1477210.1590/S1516-635X2004000100009S1516-635X2004000100009S1516-635X2004000100009.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2023-12-29T06:15:14Zoai:repositorio.unesp.br:11449/14772Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:40.322019Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
title |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
spellingShingle |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples Silva, S. E. L. [UNESP] Avibirnavirus CER cells diagnosis double antibody sandwich ELISA RT/PCR |
title_short |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
title_full |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
title_fullStr |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
title_full_unstemmed |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
title_sort |
Susceptibility of mammalian cell line for isolation of IBDV from clinical samples |
author |
Silva, S. E. L. [UNESP] |
author_facet |
Silva, S. E. L. [UNESP] Ferreira, H. L. [UNESP] Carvalho, B. A. [UNESP] Cardoso, T. C. [UNESP] |
author_role |
author |
author2 |
Ferreira, H. L. [UNESP] Carvalho, B. A. [UNESP] Cardoso, T. C. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, S. E. L. [UNESP] Ferreira, H. L. [UNESP] Carvalho, B. A. [UNESP] Cardoso, T. C. [UNESP] |
dc.subject.por.fl_str_mv |
Avibirnavirus CER cells diagnosis double antibody sandwich ELISA RT/PCR |
topic |
Avibirnavirus CER cells diagnosis double antibody sandwich ELISA RT/PCR |
description |
Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect (CPE) caused by IBDV infection was pronounced at 18h for Vero and 36h for CER cells. The RT-PCR was done to detect IBDV RNA from the bursa samples, where all were positive. Regarding to IBDV isolation, 20% of samples tested were negative in Vero cells, where all were positive in CER cells infection. The findings allow concluding that CER cell can be a new and appropriated tool for IBDV isolation. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03-01 2013-09-30T20:13:06Z 2013-09-30T20:13:06Z 2014-05-20T13:42:29Z 2014-05-20T13:42:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2004000100009 Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 1, p. 65-69, 2004. 1516-635X http://hdl.handle.net/11449/14772 10.1590/S1516-635X2004000100009 S1516-635X2004000100009 S1516-635X2004000100009.pdf |
url |
http://dx.doi.org/10.1590/S1516-635X2004000100009 http://hdl.handle.net/11449/14772 |
identifier_str_mv |
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 1, p. 65-69, 2004. 1516-635X 10.1590/S1516-635X2004000100009 S1516-635X2004000100009 S1516-635X2004000100009.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciência Avícola 0.463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
65-69 application/pdf |
dc.publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129335591174144 |