Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10529-020-03006-9 http://hdl.handle.net/11449/205216 |
Resumo: | Objective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production. |
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Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactorAspergillusEnzyme productionFructooligosaccharidesFructosyltransferaseKLaβ-fructofuranosidaseObjective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Bionanomanufacturing Center Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Vía Blanca 804, Carretera Central, San Miguel del PadrónInstitute of Science and Technology Federal University of Alfenas (ICT/UNIFAL-MG), José Aurélio Vilela Road, 11999 BR 267 Km 533Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/noInstituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/noInstituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178Institute for Technological Research (IPT)Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA)Federal University of Alfenas (ICT/UNIFAL-MG)Universidade Estadual Paulista (Unesp)Maiorano, Alfredo Eduardoda Silva, Elda SabinoPerna, Rafael FirmaniOttoni, Cristiane Angélica [UNESP]Piccoli, Rosane Aparecida MonizFernandez, Rubén CuervoMaresma, Beatriz Guilartede Andrade Rodrigues, Maria Filomena2021-06-25T10:11:47Z2021-06-25T10:11:47Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2619-2629http://dx.doi.org/10.1007/s10529-020-03006-9Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020.1573-67760141-5492http://hdl.handle.net/11449/20521610.1007/s10529-020-03006-92-s2.0-8509143896296201224557082230000-0003-4069-509XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology Lettersinfo:eu-repo/semantics/openAccess2022-03-09T00:09:58Zoai:repositorio.unesp.br:11449/205216Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:13:51.885132Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
title |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
spellingShingle |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor Maiorano, Alfredo Eduardo Aspergillus Enzyme production Fructooligosaccharides Fructosyltransferase KLa β-fructofuranosidase |
title_short |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
title_full |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
title_fullStr |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
title_full_unstemmed |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
title_sort |
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor |
author |
Maiorano, Alfredo Eduardo |
author_facet |
Maiorano, Alfredo Eduardo da Silva, Elda Sabino Perna, Rafael Firmani Ottoni, Cristiane Angélica [UNESP] Piccoli, Rosane Aparecida Moniz Fernandez, Rubén Cuervo Maresma, Beatriz Guilarte de Andrade Rodrigues, Maria Filomena |
author_role |
author |
author2 |
da Silva, Elda Sabino Perna, Rafael Firmani Ottoni, Cristiane Angélica [UNESP] Piccoli, Rosane Aparecida Moniz Fernandez, Rubén Cuervo Maresma, Beatriz Guilarte de Andrade Rodrigues, Maria Filomena |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Institute for Technological Research (IPT) Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA) Federal University of Alfenas (ICT/UNIFAL-MG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Maiorano, Alfredo Eduardo da Silva, Elda Sabino Perna, Rafael Firmani Ottoni, Cristiane Angélica [UNESP] Piccoli, Rosane Aparecida Moniz Fernandez, Rubén Cuervo Maresma, Beatriz Guilarte de Andrade Rodrigues, Maria Filomena |
dc.subject.por.fl_str_mv |
Aspergillus Enzyme production Fructooligosaccharides Fructosyltransferase KLa β-fructofuranosidase |
topic |
Aspergillus Enzyme production Fructooligosaccharides Fructosyltransferase KLa β-fructofuranosidase |
description |
Objective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T10:11:47Z 2021-06-25T10:11:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10529-020-03006-9 Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020. 1573-6776 0141-5492 http://hdl.handle.net/11449/205216 10.1007/s10529-020-03006-9 2-s2.0-85091438962 9620122455708223 0000-0003-4069-509X |
url |
http://dx.doi.org/10.1007/s10529-020-03006-9 http://hdl.handle.net/11449/205216 |
identifier_str_mv |
Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020. 1573-6776 0141-5492 10.1007/s10529-020-03006-9 2-s2.0-85091438962 9620122455708223 0000-0003-4069-509X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biotechnology Letters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2619-2629 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129500913860608 |