Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor

Detalhes bibliográficos
Autor(a) principal: Maiorano, Alfredo Eduardo
Data de Publicação: 2020
Outros Autores: da Silva, Elda Sabino, Perna, Rafael Firmani, Ottoni, Cristiane Angélica [UNESP], Piccoli, Rosane Aparecida Moniz, Fernandez, Rubén Cuervo, Maresma, Beatriz Guilarte, de Andrade Rodrigues, Maria Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10529-020-03006-9
http://hdl.handle.net/11449/205216
Resumo: Objective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.
id UNSP_1915f568c4051f9d7939d53c43362534
oai_identifier_str oai:repositorio.unesp.br:11449/205216
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactorAspergillusEnzyme productionFructooligosaccharidesFructosyltransferaseKLaβ-fructofuranosidaseObjective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Bionanomanufacturing Center Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Vía Blanca 804, Carretera Central, San Miguel del PadrónInstitute of Science and Technology Federal University of Alfenas (ICT/UNIFAL-MG), José Aurélio Vilela Road, 11999 BR 267 Km 533Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/noInstituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/noInstituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178Institute for Technological Research (IPT)Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA)Federal University of Alfenas (ICT/UNIFAL-MG)Universidade Estadual Paulista (Unesp)Maiorano, Alfredo Eduardoda Silva, Elda SabinoPerna, Rafael FirmaniOttoni, Cristiane Angélica [UNESP]Piccoli, Rosane Aparecida MonizFernandez, Rubén CuervoMaresma, Beatriz Guilartede Andrade Rodrigues, Maria Filomena2021-06-25T10:11:47Z2021-06-25T10:11:47Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2619-2629http://dx.doi.org/10.1007/s10529-020-03006-9Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020.1573-67760141-5492http://hdl.handle.net/11449/20521610.1007/s10529-020-03006-92-s2.0-8509143896296201224557082230000-0003-4069-509XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology Lettersinfo:eu-repo/semantics/openAccess2022-03-09T00:09:58Zoai:repositorio.unesp.br:11449/205216Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:13:51.885132Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
title Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
spellingShingle Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
Maiorano, Alfredo Eduardo
Aspergillus
Enzyme production
Fructooligosaccharides
Fructosyltransferase
KLa
β-fructofuranosidase
title_short Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
title_full Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
title_fullStr Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
title_full_unstemmed Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
title_sort Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
author Maiorano, Alfredo Eduardo
author_facet Maiorano, Alfredo Eduardo
da Silva, Elda Sabino
Perna, Rafael Firmani
Ottoni, Cristiane Angélica [UNESP]
Piccoli, Rosane Aparecida Moniz
Fernandez, Rubén Cuervo
Maresma, Beatriz Guilarte
de Andrade Rodrigues, Maria Filomena
author_role author
author2 da Silva, Elda Sabino
Perna, Rafael Firmani
Ottoni, Cristiane Angélica [UNESP]
Piccoli, Rosane Aparecida Moniz
Fernandez, Rubén Cuervo
Maresma, Beatriz Guilarte
de Andrade Rodrigues, Maria Filomena
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institute for Technological Research (IPT)
Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA)
Federal University of Alfenas (ICT/UNIFAL-MG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Maiorano, Alfredo Eduardo
da Silva, Elda Sabino
Perna, Rafael Firmani
Ottoni, Cristiane Angélica [UNESP]
Piccoli, Rosane Aparecida Moniz
Fernandez, Rubén Cuervo
Maresma, Beatriz Guilarte
de Andrade Rodrigues, Maria Filomena
dc.subject.por.fl_str_mv Aspergillus
Enzyme production
Fructooligosaccharides
Fructosyltransferase
KLa
β-fructofuranosidase
topic Aspergillus
Enzyme production
Fructooligosaccharides
Fructosyltransferase
KLa
β-fructofuranosidase
description Objective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01
2021-06-25T10:11:47Z
2021-06-25T10:11:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10529-020-03006-9
Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020.
1573-6776
0141-5492
http://hdl.handle.net/11449/205216
10.1007/s10529-020-03006-9
2-s2.0-85091438962
9620122455708223
0000-0003-4069-509X
url http://dx.doi.org/10.1007/s10529-020-03006-9
http://hdl.handle.net/11449/205216
identifier_str_mv Biotechnology Letters, v. 42, n. 12, p. 2619-2629, 2020.
1573-6776
0141-5492
10.1007/s10529-020-03006-9
2-s2.0-85091438962
9620122455708223
0000-0003-4069-509X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biotechnology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2619-2629
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129500913860608