SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

Detalhes bibliográficos
Autor(a) principal: Cunha, Josivan S.
Data de Publicação: 2019
Outros Autores: Ottoni, Cristiane A. [UNESP], Morales, Sergio A., Silva, Elda S., Maiorano, Alfredo E., Perna, Rafael F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0104-6632.20190362s20180572
http://hdl.handle.net/11449/196217
Resumo: Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.
id UNSP_87938ecdf59631462a25c4ee81a5d82e
oai_identifier_str oai:repositorio.unesp.br:11449/196217
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTIONAspergillus oryzae IPT-301Submerged fermentationFructosyltransferaseEnzymatic characterizationFructooligosaccharidesFructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Institute for Technological Research (IPT/SP)/Programa Novos TalentosUniv Fed Alfenas, Inst Ciencia & Tecnol, Pocos De Caldas, MG, BrazilUniv Estadual Paulista, Inst Biociencias, Sao Vicente, SP, BrazilInst Pesquisas Tecnol, Ctr Bionanomanufatura, Sao Paulo, SP, BrazilUniv Minho, Ctr Engn Biol, Braga, PortugalUniv Estadual Paulista, Inst Biociencias, Sao Vicente, SP, BrazilFAPEMIG: APQ-02131-14Brazilian Soc Chemical EngUniv Fed AlfenasUniversidade Estadual Paulista (Unesp)Inst Pesquisas TecnolUniv MinhoCunha, Josivan S.Ottoni, Cristiane A. [UNESP]Morales, Sergio A.Silva, Elda S.Maiorano, Alfredo E.Perna, Rafael F.2020-12-10T19:37:27Z2020-12-10T19:37:27Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article657-668application/pdfhttp://dx.doi.org/10.1590/0104-6632.20190362s20180572Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019.0104-6632http://hdl.handle.net/11449/19621710.1590/0104-6632.20190362s20180572S0104-66322019000200657WOS:000488278700005S0104-66322019000200657.pdf96201224557082230000-0003-4069-509XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Chemical Engineeringinfo:eu-repo/semantics/openAccess2023-12-13T06:20:03Zoai:repositorio.unesp.br:11449/196217Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:13:41.993194Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
title SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
spellingShingle SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
Cunha, Josivan S.
Aspergillus oryzae IPT-301
Submerged fermentation
Fructosyltransferase
Enzymatic characterization
Fructooligosaccharides
title_short SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
title_full SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
title_fullStr SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
title_full_unstemmed SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
title_sort SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
author Cunha, Josivan S.
author_facet Cunha, Josivan S.
Ottoni, Cristiane A. [UNESP]
Morales, Sergio A.
Silva, Elda S.
Maiorano, Alfredo E.
Perna, Rafael F.
author_role author
author2 Ottoni, Cristiane A. [UNESP]
Morales, Sergio A.
Silva, Elda S.
Maiorano, Alfredo E.
Perna, Rafael F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Alfenas
Universidade Estadual Paulista (Unesp)
Inst Pesquisas Tecnol
Univ Minho
dc.contributor.author.fl_str_mv Cunha, Josivan S.
Ottoni, Cristiane A. [UNESP]
Morales, Sergio A.
Silva, Elda S.
Maiorano, Alfredo E.
Perna, Rafael F.
dc.subject.por.fl_str_mv Aspergillus oryzae IPT-301
Submerged fermentation
Fructosyltransferase
Enzymatic characterization
Fructooligosaccharides
topic Aspergillus oryzae IPT-301
Submerged fermentation
Fructosyltransferase
Enzymatic characterization
Fructooligosaccharides
description Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-01
2020-12-10T19:37:27Z
2020-12-10T19:37:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0104-6632.20190362s20180572
Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019.
0104-6632
http://hdl.handle.net/11449/196217
10.1590/0104-6632.20190362s20180572
S0104-66322019000200657
WOS:000488278700005
S0104-66322019000200657.pdf
9620122455708223
0000-0003-4069-509X
url http://dx.doi.org/10.1590/0104-6632.20190362s20180572
http://hdl.handle.net/11449/196217
identifier_str_mv Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019.
0104-6632
10.1590/0104-6632.20190362s20180572
S0104-66322019000200657
WOS:000488278700005
S0104-66322019000200657.pdf
9620122455708223
0000-0003-4069-509X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Chemical Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 657-668
application/pdf
dc.publisher.none.fl_str_mv Brazilian Soc Chemical Eng
publisher.none.fl_str_mv Brazilian Soc Chemical Eng
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129176030412800