SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0104-6632.20190362s20180572 http://hdl.handle.net/11449/196217 |
Resumo: | Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale. |
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Repositório Institucional da UNESP |
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SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTIONAspergillus oryzae IPT-301Submerged fermentationFructosyltransferaseEnzymatic characterizationFructooligosaccharidesFructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Institute for Technological Research (IPT/SP)/Programa Novos TalentosUniv Fed Alfenas, Inst Ciencia & Tecnol, Pocos De Caldas, MG, BrazilUniv Estadual Paulista, Inst Biociencias, Sao Vicente, SP, BrazilInst Pesquisas Tecnol, Ctr Bionanomanufatura, Sao Paulo, SP, BrazilUniv Minho, Ctr Engn Biol, Braga, PortugalUniv Estadual Paulista, Inst Biociencias, Sao Vicente, SP, BrazilFAPEMIG: APQ-02131-14Brazilian Soc Chemical EngUniv Fed AlfenasUniversidade Estadual Paulista (Unesp)Inst Pesquisas TecnolUniv MinhoCunha, Josivan S.Ottoni, Cristiane A. [UNESP]Morales, Sergio A.Silva, Elda S.Maiorano, Alfredo E.Perna, Rafael F.2020-12-10T19:37:27Z2020-12-10T19:37:27Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article657-668application/pdfhttp://dx.doi.org/10.1590/0104-6632.20190362s20180572Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019.0104-6632http://hdl.handle.net/11449/19621710.1590/0104-6632.20190362s20180572S0104-66322019000200657WOS:000488278700005S0104-66322019000200657.pdf96201224557082230000-0003-4069-509XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Chemical Engineeringinfo:eu-repo/semantics/openAccess2023-12-13T06:20:03Zoai:repositorio.unesp.br:11449/196217Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:13:41.993194Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
title |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
spellingShingle |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION Cunha, Josivan S. Aspergillus oryzae IPT-301 Submerged fermentation Fructosyltransferase Enzymatic characterization Fructooligosaccharides |
title_short |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
title_full |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
title_fullStr |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
title_full_unstemmed |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
title_sort |
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION |
author |
Cunha, Josivan S. |
author_facet |
Cunha, Josivan S. Ottoni, Cristiane A. [UNESP] Morales, Sergio A. Silva, Elda S. Maiorano, Alfredo E. Perna, Rafael F. |
author_role |
author |
author2 |
Ottoni, Cristiane A. [UNESP] Morales, Sergio A. Silva, Elda S. Maiorano, Alfredo E. Perna, Rafael F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Alfenas Universidade Estadual Paulista (Unesp) Inst Pesquisas Tecnol Univ Minho |
dc.contributor.author.fl_str_mv |
Cunha, Josivan S. Ottoni, Cristiane A. [UNESP] Morales, Sergio A. Silva, Elda S. Maiorano, Alfredo E. Perna, Rafael F. |
dc.subject.por.fl_str_mv |
Aspergillus oryzae IPT-301 Submerged fermentation Fructosyltransferase Enzymatic characterization Fructooligosaccharides |
topic |
Aspergillus oryzae IPT-301 Submerged fermentation Fructosyltransferase Enzymatic characterization Fructooligosaccharides |
description |
Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 2020-12-10T19:37:27Z 2020-12-10T19:37:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0104-6632.20190362s20180572 Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019. 0104-6632 http://hdl.handle.net/11449/196217 10.1590/0104-6632.20190362s20180572 S0104-66322019000200657 WOS:000488278700005 S0104-66322019000200657.pdf 9620122455708223 0000-0003-4069-509X |
url |
http://dx.doi.org/10.1590/0104-6632.20190362s20180572 http://hdl.handle.net/11449/196217 |
identifier_str_mv |
Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019. 0104-6632 10.1590/0104-6632.20190362s20180572 S0104-66322019000200657 WOS:000488278700005 S0104-66322019000200657.pdf 9620122455708223 0000-0003-4069-509X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Chemical Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
657-668 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Soc Chemical Eng |
publisher.none.fl_str_mv |
Brazilian Soc Chemical Eng |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129176030412800 |