Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2016.11.010 http://hdl.handle.net/11449/174196 |
Resumo: | We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. |
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Repositório Institucional da UNESP |
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Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping HorsesCD 147Exercise physiologyLactateLactate transporter complexMCT1We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.Faculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de Morfologia e Fisiologia Animal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de TecnologiaBrazilian ArmyFaculdades Metropolitanas Unidas HOVETFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de Morfologia e Fisiologia Animal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de TecnologiaUniversidade Estadual Paulista (Unesp)Brazilian ArmyHOVETFeringer Júnior, Walter Heinz [UNESP]de Carvalho, Julia Ribeiro Garcia [UNESP]de Almeida, Maria Luiza Mendes [UNESP]Lemos, Eliana Gertrudes Macedo [UNESP]Soares, Otávio Augusto BrioschiRibeiro, Gesianede Queiroz-Neto, Antonio [UNESP]de Camargo Ferraz, Guilherme [UNESP]2018-12-11T17:09:47Z2018-12-11T17:09:47Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-144application/pdfhttp://dx.doi.org/10.1016/j.jevs.2016.11.010Journal of Equine Veterinary Science, v. 50, p. 139-144.0737-0806http://hdl.handle.net/11449/17419610.1016/j.jevs.2016.11.0102-s2.0-850121366302-s2.0-85012136630.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0,390info:eu-repo/semantics/openAccess2024-06-07T15:31:16Zoai:repositorio.unesp.br:11449/174196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:21:56.094573Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
spellingShingle |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses Feringer Júnior, Walter Heinz [UNESP] CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
title_short |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_full |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_fullStr |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_full_unstemmed |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_sort |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
author |
Feringer Júnior, Walter Heinz [UNESP] |
author_facet |
Feringer Júnior, Walter Heinz [UNESP] de Carvalho, Julia Ribeiro Garcia [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
author_role |
author |
author2 |
de Carvalho, Julia Ribeiro Garcia [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Brazilian Army HOVET |
dc.contributor.author.fl_str_mv |
Feringer Júnior, Walter Heinz [UNESP] de Carvalho, Julia Ribeiro Garcia [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
topic |
CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
description |
We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:09:47Z 2018-12-11T17:09:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2016.11.010 Journal of Equine Veterinary Science, v. 50, p. 139-144. 0737-0806 http://hdl.handle.net/11449/174196 10.1016/j.jevs.2016.11.010 2-s2.0-85012136630 2-s2.0-85012136630.pdf |
url |
http://dx.doi.org/10.1016/j.jevs.2016.11.010 http://hdl.handle.net/11449/174196 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 50, p. 139-144. 0737-0806 10.1016/j.jevs.2016.11.010 2-s2.0-85012136630 2-s2.0-85012136630.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science 0,390 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
139-144 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128353034567680 |