Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

Detalhes bibliográficos
Autor(a) principal: Feringer Júnior, Walter Heinz [UNESP]
Data de Publicação: 2017
Outros Autores: de Carvalho, Julia Ribeiro Garcia [UNESP], de Almeida, Maria Luiza Mendes [UNESP], Lemos, Eliana Gertrudes Macedo [UNESP], Soares, Otávio Augusto Brioschi, Ribeiro, Gesiane, de Queiroz-Neto, Antonio [UNESP], de Camargo Ferraz, Guilherme [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jevs.2016.11.010
http://hdl.handle.net/11449/174196
Resumo: We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.
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spelling Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping HorsesCD 147Exercise physiologyLactateLactate transporter complexMCT1We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.Faculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de Morfologia e Fisiologia Animal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de TecnologiaBrazilian ArmyFaculdades Metropolitanas Unidas HOVETFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de Morfologia e Fisiologia Animal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Departamento de TecnologiaUniversidade Estadual Paulista (Unesp)Brazilian ArmyHOVETFeringer Júnior, Walter Heinz [UNESP]de Carvalho, Julia Ribeiro Garcia [UNESP]de Almeida, Maria Luiza Mendes [UNESP]Lemos, Eliana Gertrudes Macedo [UNESP]Soares, Otávio Augusto BrioschiRibeiro, Gesianede Queiroz-Neto, Antonio [UNESP]de Camargo Ferraz, Guilherme [UNESP]2018-12-11T17:09:47Z2018-12-11T17:09:47Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-144application/pdfhttp://dx.doi.org/10.1016/j.jevs.2016.11.010Journal of Equine Veterinary Science, v. 50, p. 139-144.0737-0806http://hdl.handle.net/11449/17419610.1016/j.jevs.2016.11.0102-s2.0-850121366302-s2.0-85012136630.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0,390info:eu-repo/semantics/openAccess2024-06-07T15:31:16Zoai:repositorio.unesp.br:11449/174196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:21:56.094573Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
spellingShingle Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
Feringer Júnior, Walter Heinz [UNESP]
CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
title_short Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_full Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_fullStr Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_full_unstemmed Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_sort Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
author Feringer Júnior, Walter Heinz [UNESP]
author_facet Feringer Júnior, Walter Heinz [UNESP]
de Carvalho, Julia Ribeiro Garcia [UNESP]
de Almeida, Maria Luiza Mendes [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Soares, Otávio Augusto Brioschi
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
author_role author
author2 de Carvalho, Julia Ribeiro Garcia [UNESP]
de Almeida, Maria Luiza Mendes [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Soares, Otávio Augusto Brioschi
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Brazilian Army
HOVET
dc.contributor.author.fl_str_mv Feringer Júnior, Walter Heinz [UNESP]
de Carvalho, Julia Ribeiro Garcia [UNESP]
de Almeida, Maria Luiza Mendes [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Soares, Otávio Augusto Brioschi
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
dc.subject.por.fl_str_mv CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
topic CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
description We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
2018-12-11T17:09:47Z
2018-12-11T17:09:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jevs.2016.11.010
Journal of Equine Veterinary Science, v. 50, p. 139-144.
0737-0806
http://hdl.handle.net/11449/174196
10.1016/j.jevs.2016.11.010
2-s2.0-85012136630
2-s2.0-85012136630.pdf
url http://dx.doi.org/10.1016/j.jevs.2016.11.010
http://hdl.handle.net/11449/174196
identifier_str_mv Journal of Equine Veterinary Science, v. 50, p. 139-144.
0737-0806
10.1016/j.jevs.2016.11.010
2-s2.0-85012136630
2-s2.0-85012136630.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Equine Veterinary Science
0,390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 139-144
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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