Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

Detalhes bibliográficos
Autor(a) principal: Júnior, Walter Heinz Feringer [UNESP]
Data de Publicação: 2019
Outros Autores: Garcia de Carvalho, Julia Ribeiro [UNESP], Mendes de Almeida, Maria Luiza [UNESP], Macedo Lemos, Eliana Gertrudes [UNESP], Brioschi Soares, Otávio Augusto, Ribeiro, Gesiane, de Queiroz-Neto, Antonio [UNESP], de Camargo Ferraz, Guilherme [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jevs.2019.102791
http://hdl.handle.net/11449/199462
Resumo: We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.
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spelling Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping HorsesCD 147Exercise physiologyLactateLactate transporter complexMCT1We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.Departamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de JaboticabalBrazilain ArmyFaculdades Metropolitanas Unidas HOVETDepartamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de JaboticabalUniversidade Estadual Paulista (Unesp)Brazilain ArmyHOVETJúnior, Walter Heinz Feringer [UNESP]Garcia de Carvalho, Julia Ribeiro [UNESP]Mendes de Almeida, Maria Luiza [UNESP]Macedo Lemos, Eliana Gertrudes [UNESP]Brioschi Soares, Otávio AugustoRibeiro, Gesianede Queiroz-Neto, Antonio [UNESP]de Camargo Ferraz, Guilherme [UNESP]2020-12-12T01:40:30Z2020-12-12T01:40:30Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jevs.2019.102791Journal of Equine Veterinary Science, v. 81.0737-0806http://hdl.handle.net/11449/19946210.1016/j.jevs.2019.1027912-s2.0-85072884580Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-06-07T15:32:48Zoai:repositorio.unesp.br:11449/199462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:25.229444Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
spellingShingle Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
Júnior, Walter Heinz Feringer [UNESP]
CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
title_short Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_full Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_fullStr Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_full_unstemmed Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
title_sort Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
author Júnior, Walter Heinz Feringer [UNESP]
author_facet Júnior, Walter Heinz Feringer [UNESP]
Garcia de Carvalho, Julia Ribeiro [UNESP]
Mendes de Almeida, Maria Luiza [UNESP]
Macedo Lemos, Eliana Gertrudes [UNESP]
Brioschi Soares, Otávio Augusto
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
author_role author
author2 Garcia de Carvalho, Julia Ribeiro [UNESP]
Mendes de Almeida, Maria Luiza [UNESP]
Macedo Lemos, Eliana Gertrudes [UNESP]
Brioschi Soares, Otávio Augusto
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Brazilain Army
HOVET
dc.contributor.author.fl_str_mv Júnior, Walter Heinz Feringer [UNESP]
Garcia de Carvalho, Julia Ribeiro [UNESP]
Mendes de Almeida, Maria Luiza [UNESP]
Macedo Lemos, Eliana Gertrudes [UNESP]
Brioschi Soares, Otávio Augusto
Ribeiro, Gesiane
de Queiroz-Neto, Antonio [UNESP]
de Camargo Ferraz, Guilherme [UNESP]
dc.subject.por.fl_str_mv CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
topic CD 147
Exercise physiology
Lactate
Lactate transporter complex
MCT1
description We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-01
2020-12-12T01:40:30Z
2020-12-12T01:40:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jevs.2019.102791
Journal of Equine Veterinary Science, v. 81.
0737-0806
http://hdl.handle.net/11449/199462
10.1016/j.jevs.2019.102791
2-s2.0-85072884580
url http://dx.doi.org/10.1016/j.jevs.2019.102791
http://hdl.handle.net/11449/199462
identifier_str_mv Journal of Equine Veterinary Science, v. 81.
0737-0806
10.1016/j.jevs.2019.102791
2-s2.0-85072884580
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Equine Veterinary Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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