Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2019.102791 http://hdl.handle.net/11449/199462 |
Resumo: | We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. |
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Repositório Institucional da UNESP |
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Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping HorsesCD 147Exercise physiologyLactateLactate transporter complexMCT1We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.Departamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de JaboticabalBrazilain ArmyFaculdades Metropolitanas Unidas HOVETDepartamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) JaboticabalDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de JaboticabalUniversidade Estadual Paulista (Unesp)Brazilain ArmyHOVETJúnior, Walter Heinz Feringer [UNESP]Garcia de Carvalho, Julia Ribeiro [UNESP]Mendes de Almeida, Maria Luiza [UNESP]Macedo Lemos, Eliana Gertrudes [UNESP]Brioschi Soares, Otávio AugustoRibeiro, Gesianede Queiroz-Neto, Antonio [UNESP]de Camargo Ferraz, Guilherme [UNESP]2020-12-12T01:40:30Z2020-12-12T01:40:30Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jevs.2019.102791Journal of Equine Veterinary Science, v. 81.0737-0806http://hdl.handle.net/11449/19946210.1016/j.jevs.2019.1027912-s2.0-85072884580Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-06-07T15:32:48Zoai:repositorio.unesp.br:11449/199462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:25.229444Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
spellingShingle |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses Júnior, Walter Heinz Feringer [UNESP] CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
title_short |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_full |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_fullStr |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_full_unstemmed |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
title_sort |
Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses |
author |
Júnior, Walter Heinz Feringer [UNESP] |
author_facet |
Júnior, Walter Heinz Feringer [UNESP] Garcia de Carvalho, Julia Ribeiro [UNESP] Mendes de Almeida, Maria Luiza [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] Brioschi Soares, Otávio Augusto Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
author_role |
author |
author2 |
Garcia de Carvalho, Julia Ribeiro [UNESP] Mendes de Almeida, Maria Luiza [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] Brioschi Soares, Otávio Augusto Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Brazilain Army HOVET |
dc.contributor.author.fl_str_mv |
Júnior, Walter Heinz Feringer [UNESP] Garcia de Carvalho, Julia Ribeiro [UNESP] Mendes de Almeida, Maria Luiza [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] Brioschi Soares, Otávio Augusto Ribeiro, Gesiane de Queiroz-Neto, Antonio [UNESP] de Camargo Ferraz, Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
topic |
CD 147 Exercise physiology Lactate Lactate transporter complex MCT1 |
description |
We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 2020-12-12T01:40:30Z 2020-12-12T01:40:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2019.102791 Journal of Equine Veterinary Science, v. 81. 0737-0806 http://hdl.handle.net/11449/199462 10.1016/j.jevs.2019.102791 2-s2.0-85072884580 |
url |
http://dx.doi.org/10.1016/j.jevs.2019.102791 http://hdl.handle.net/11449/199462 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 81. 0737-0806 10.1016/j.jevs.2019.102791 2-s2.0-85072884580 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129533916741632 |