Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2022.102795 http://hdl.handle.net/11449/230628 |
Resumo: | Background: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions. |
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Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizerCurcuminCytotoxicityFibroblastsPhotodynamic therapyRoot canal irrigantsRoot canal therapyBackground: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions.Department of Dental Materials and Prosthodontics Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Preventive and Restorative Dentistry Discipline of Endodontics Araçatuba School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Surgery and Integrated Clinic Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Basic Sciences Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Dental Materials and Prosthodontics Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Preventive and Restorative Dentistry Discipline of Endodontics Araçatuba School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Surgery and Integrated Clinic Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Basic Sciences Araçatuba School of Dentistry São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Strazzi-Sahyon, H. B. [UNESP]Cintra, L. T.A. [UNESP]Nakao, J. M. [UNESP]Takamiya, A. S. [UNESP]Queiroz, I. O.A. [UNESP]Dos Santos, P. H. [UNESP]Oliveira, Sandra Helena Penha de [UNESP]Sivieri-Araujo, G. [UNESP]2022-04-29T08:41:17Z2022-04-29T08:41:17Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2022.102795Photodiagnosis and Photodynamic Therapy, v. 38.1873-15971572-1000http://hdl.handle.net/11449/23062810.1016/j.pdpdt.2022.1027952-s2.0-85126970252Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2024-09-19T18:31:13Zoai:repositorio.unesp.br:11449/230628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
title |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
spellingShingle |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer Strazzi-Sahyon, H. B. [UNESP] Curcumin Cytotoxicity Fibroblasts Photodynamic therapy Root canal irrigants Root canal therapy |
title_short |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
title_full |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
title_fullStr |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
title_full_unstemmed |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
title_sort |
Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer |
author |
Strazzi-Sahyon, H. B. [UNESP] |
author_facet |
Strazzi-Sahyon, H. B. [UNESP] Cintra, L. T.A. [UNESP] Nakao, J. M. [UNESP] Takamiya, A. S. [UNESP] Queiroz, I. O.A. [UNESP] Dos Santos, P. H. [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Sivieri-Araujo, G. [UNESP] |
author_role |
author |
author2 |
Cintra, L. T.A. [UNESP] Nakao, J. M. [UNESP] Takamiya, A. S. [UNESP] Queiroz, I. O.A. [UNESP] Dos Santos, P. H. [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Sivieri-Araujo, G. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Strazzi-Sahyon, H. B. [UNESP] Cintra, L. T.A. [UNESP] Nakao, J. M. [UNESP] Takamiya, A. S. [UNESP] Queiroz, I. O.A. [UNESP] Dos Santos, P. H. [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Sivieri-Araujo, G. [UNESP] |
dc.subject.por.fl_str_mv |
Curcumin Cytotoxicity Fibroblasts Photodynamic therapy Root canal irrigants Root canal therapy |
topic |
Curcumin Cytotoxicity Fibroblasts Photodynamic therapy Root canal irrigants Root canal therapy |
description |
Background: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:41:17Z 2022-04-29T08:41:17Z 2022-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2022.102795 Photodiagnosis and Photodynamic Therapy, v. 38. 1873-1597 1572-1000 http://hdl.handle.net/11449/230628 10.1016/j.pdpdt.2022.102795 2-s2.0-85126970252 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2022.102795 http://hdl.handle.net/11449/230628 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 38. 1873-1597 1572-1000 10.1016/j.pdpdt.2022.102795 2-s2.0-85126970252 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546379532828672 |