Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer

Detalhes bibliográficos
Autor(a) principal: Strazzi-Sahyon, H. B. [UNESP]
Data de Publicação: 2022
Outros Autores: Cintra, L. T.A. [UNESP], Nakao, J. M. [UNESP], Takamiya, A. S. [UNESP], Queiroz, I. O.A. [UNESP], Dos Santos, P. H. [UNESP], Oliveira, Sandra Helena Penha de [UNESP], Sivieri-Araujo, G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2022.102795
http://hdl.handle.net/11449/230628
Resumo: Background: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions.
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spelling Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizerCurcuminCytotoxicityFibroblastsPhotodynamic therapyRoot canal irrigantsRoot canal therapyBackground: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions.Department of Dental Materials and Prosthodontics Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Preventive and Restorative Dentistry Discipline of Endodontics Araçatuba School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Surgery and Integrated Clinic Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Basic Sciences Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Dental Materials and Prosthodontics Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Preventive and Restorative Dentistry Discipline of Endodontics Araçatuba School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Surgery and Integrated Clinic Araçatuba School of Dentistry São Paulo State University (UNESP), SPDepartment of Basic Sciences Araçatuba School of Dentistry São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Strazzi-Sahyon, H. B. [UNESP]Cintra, L. T.A. [UNESP]Nakao, J. M. [UNESP]Takamiya, A. S. [UNESP]Queiroz, I. O.A. [UNESP]Dos Santos, P. H. [UNESP]Oliveira, Sandra Helena Penha de [UNESP]Sivieri-Araujo, G. [UNESP]2022-04-29T08:41:17Z2022-04-29T08:41:17Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2022.102795Photodiagnosis and Photodynamic Therapy, v. 38.1873-15971572-1000http://hdl.handle.net/11449/23062810.1016/j.pdpdt.2022.1027952-s2.0-85126970252Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2024-09-19T18:31:13Zoai:repositorio.unesp.br:11449/230628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
title Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
spellingShingle Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
Strazzi-Sahyon, H. B. [UNESP]
Curcumin
Cytotoxicity
Fibroblasts
Photodynamic therapy
Root canal irrigants
Root canal therapy
title_short Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
title_full Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
title_fullStr Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
title_full_unstemmed Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
title_sort Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer
author Strazzi-Sahyon, H. B. [UNESP]
author_facet Strazzi-Sahyon, H. B. [UNESP]
Cintra, L. T.A. [UNESP]
Nakao, J. M. [UNESP]
Takamiya, A. S. [UNESP]
Queiroz, I. O.A. [UNESP]
Dos Santos, P. H. [UNESP]
Oliveira, Sandra Helena Penha de [UNESP]
Sivieri-Araujo, G. [UNESP]
author_role author
author2 Cintra, L. T.A. [UNESP]
Nakao, J. M. [UNESP]
Takamiya, A. S. [UNESP]
Queiroz, I. O.A. [UNESP]
Dos Santos, P. H. [UNESP]
Oliveira, Sandra Helena Penha de [UNESP]
Sivieri-Araujo, G. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Strazzi-Sahyon, H. B. [UNESP]
Cintra, L. T.A. [UNESP]
Nakao, J. M. [UNESP]
Takamiya, A. S. [UNESP]
Queiroz, I. O.A. [UNESP]
Dos Santos, P. H. [UNESP]
Oliveira, Sandra Helena Penha de [UNESP]
Sivieri-Araujo, G. [UNESP]
dc.subject.por.fl_str_mv Curcumin
Cytotoxicity
Fibroblasts
Photodynamic therapy
Root canal irrigants
Root canal therapy
topic Curcumin
Cytotoxicity
Fibroblasts
Photodynamic therapy
Root canal irrigants
Root canal therapy
description Background: Photodynamic therapy (PDT) has shown satisfactory antibacterial effects. However, little information regarding the cytotoxicity potential of PDT using curcumin as a photosensitizer (PS) on fibroblasts are found. The aim of this in vitro study was to evaluate the cytotoxicity of root canal irrigating solutions and photodynamic therapy with curcumin PS on the L-929 cell line. Methods: Healthy mouse skin fibroblast cells were distributed into the following 7 experimental groups: G1 – culture medium DMEM (control group); G2 – 0.9% sodium chloride; G3 – 2.5% sodium hypochlorite (NaOCl); G4 – 5% NaOCl; G5 – PDT with curcumin PS at 500 mg/L + blue LED; G6 – PDT with curcumin PS at 750 mg/L + blue LED; and G7 - PDT with curcumin PS at 1000 mg/L + blue LED. All experimental groups which underwent PDT action were submitted to blue LED for 4 min, with a wavelength of 480 nm and energy fluency of 75 J/cm². The cultures were maintained under standard cell culture conditions (37°C, 100% humidity, 5% CO2). Cell viability analysis was performed using the colorimetric method to evaluate the periods of 6, 24, and 48 h. Data were subjected to the Kruskal–Wallis test, followed by the Dunn test to compare groups and Friedman test to compare periods (α = 0.05). Results: When comparing the periods, no significant differences were observed for any of the experimental groups analyzed (p > 0.05), except for the NaOCl2.5 group that exhibited higher cell viability at 6 h compared to the period of 48 h (p = 0.0489). In the comparisons of the experimental groups, there were no statistically significant differences between the control group compared to all disinfection protocols, regardless of the period evaluated (p > 0.05), except for the PDT + C1000 group that showed lower cell viability (P < 0.05). Conclusions: PDT with curcumin at 1000 mg/L was cytotoxic on L-929 fibroblast cell culture. However, laser-activated curcumin at a concentration of 500 mg/L presented no influence on L-929 fibroblast cell viability in in vitro conditions.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:41:17Z
2022-04-29T08:41:17Z
2022-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2022.102795
Photodiagnosis and Photodynamic Therapy, v. 38.
1873-1597
1572-1000
http://hdl.handle.net/11449/230628
10.1016/j.pdpdt.2022.102795
2-s2.0-85126970252
url http://dx.doi.org/10.1016/j.pdpdt.2022.102795
http://hdl.handle.net/11449/230628
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 38.
1873-1597
1572-1000
10.1016/j.pdpdt.2022.102795
2-s2.0-85126970252
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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