Evaluation of photodynamic therapy on fibroblast viability and cytokine production

Detalhes bibliográficos
Autor(a) principal: Gomes-Filho, Joao Eduardo [UNESP]
Data de Publicação: 2016
Outros Autores: Sivieri-Araujo, Gustavo [UNESP], Sipert, Carla Renata, Silva Santos, Ludmilla Mota da [UNESP], Azevedo Queiroz, India Olinta de [UNESP], Martins, Christine Men [UNESP], Carmo Maia, Nayara Kivilla do [UNESP], Angelo Cintra, Luciano Tavares [UNESP], Dezan-Junior, Eloi [UNESP], Bagnato, Vanderlei Salvador, Chaves-Neto, Antonio Hernandes [UNESP], Penha de Oliveira, Sandra Helena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2016.01.007
http://hdl.handle.net/11449/161333
Resumo: Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved.
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spelling Evaluation of photodynamic therapy on fibroblast viability and cytokine productionEndodontic treatmentRoot canal irrigantsPhotodynamic therapyCytotoxicityCytokineBackground: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP, BrazilUniv Sao Paulo, Sch Dent, Discipline Endodont, Dept Restorat Dent, Sao Paulo, SP, BrazilUniv Sao Paulo, Phys Inst Sao Carlos, Opt Grp, Sao Carlos, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP, BrazilFAPESP: 2011/23287-8FAPESP: 2012/12695-0FAPESP: 2012/06785-7Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Gomes-Filho, Joao Eduardo [UNESP]Sivieri-Araujo, Gustavo [UNESP]Sipert, Carla RenataSilva Santos, Ludmilla Mota da [UNESP]Azevedo Queiroz, India Olinta de [UNESP]Martins, Christine Men [UNESP]Carmo Maia, Nayara Kivilla do [UNESP]Angelo Cintra, Luciano Tavares [UNESP]Dezan-Junior, Eloi [UNESP]Bagnato, Vanderlei SalvadorChaves-Neto, Antonio Hernandes [UNESP]Penha de Oliveira, Sandra Helena [UNESP]2018-11-26T16:28:05Z2018-11-26T16:28:05Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-100application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2016.01.007Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016.1572-1000http://hdl.handle.net/11449/16133310.1016/j.pdpdt.2016.01.007WOS:000372376500015WOS000372376500015.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis And Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2024-09-19T18:31:51Zoai:repositorio.unesp.br:11449/161333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of photodynamic therapy on fibroblast viability and cytokine production
title Evaluation of photodynamic therapy on fibroblast viability and cytokine production
spellingShingle Evaluation of photodynamic therapy on fibroblast viability and cytokine production
Gomes-Filho, Joao Eduardo [UNESP]
Endodontic treatment
Root canal irrigants
Photodynamic therapy
Cytotoxicity
Cytokine
title_short Evaluation of photodynamic therapy on fibroblast viability and cytokine production
title_full Evaluation of photodynamic therapy on fibroblast viability and cytokine production
title_fullStr Evaluation of photodynamic therapy on fibroblast viability and cytokine production
title_full_unstemmed Evaluation of photodynamic therapy on fibroblast viability and cytokine production
title_sort Evaluation of photodynamic therapy on fibroblast viability and cytokine production
author Gomes-Filho, Joao Eduardo [UNESP]
author_facet Gomes-Filho, Joao Eduardo [UNESP]
Sivieri-Araujo, Gustavo [UNESP]
Sipert, Carla Renata
Silva Santos, Ludmilla Mota da [UNESP]
Azevedo Queiroz, India Olinta de [UNESP]
Martins, Christine Men [UNESP]
Carmo Maia, Nayara Kivilla do [UNESP]
Angelo Cintra, Luciano Tavares [UNESP]
Dezan-Junior, Eloi [UNESP]
Bagnato, Vanderlei Salvador
Chaves-Neto, Antonio Hernandes [UNESP]
Penha de Oliveira, Sandra Helena [UNESP]
author_role author
author2 Sivieri-Araujo, Gustavo [UNESP]
Sipert, Carla Renata
Silva Santos, Ludmilla Mota da [UNESP]
Azevedo Queiroz, India Olinta de [UNESP]
Martins, Christine Men [UNESP]
Carmo Maia, Nayara Kivilla do [UNESP]
Angelo Cintra, Luciano Tavares [UNESP]
Dezan-Junior, Eloi [UNESP]
Bagnato, Vanderlei Salvador
Chaves-Neto, Antonio Hernandes [UNESP]
Penha de Oliveira, Sandra Helena [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Gomes-Filho, Joao Eduardo [UNESP]
Sivieri-Araujo, Gustavo [UNESP]
Sipert, Carla Renata
Silva Santos, Ludmilla Mota da [UNESP]
Azevedo Queiroz, India Olinta de [UNESP]
Martins, Christine Men [UNESP]
Carmo Maia, Nayara Kivilla do [UNESP]
Angelo Cintra, Luciano Tavares [UNESP]
Dezan-Junior, Eloi [UNESP]
Bagnato, Vanderlei Salvador
Chaves-Neto, Antonio Hernandes [UNESP]
Penha de Oliveira, Sandra Helena [UNESP]
dc.subject.por.fl_str_mv Endodontic treatment
Root canal irrigants
Photodynamic therapy
Cytotoxicity
Cytokine
topic Endodontic treatment
Root canal irrigants
Photodynamic therapy
Cytotoxicity
Cytokine
description Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
2018-11-26T16:28:05Z
2018-11-26T16:28:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2016.01.007
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016.
1572-1000
http://hdl.handle.net/11449/161333
10.1016/j.pdpdt.2016.01.007
WOS:000372376500015
WOS000372376500015.pdf
url http://dx.doi.org/10.1016/j.pdpdt.2016.01.007
http://hdl.handle.net/11449/161333
identifier_str_mv Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016.
1572-1000
10.1016/j.pdpdt.2016.01.007
WOS:000372376500015
WOS000372376500015.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis And Photodynamic Therapy
0,647
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 97-100
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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