Evaluation of photodynamic therapy on fibroblast viability and cytokine production
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2016.01.007 http://hdl.handle.net/11449/161333 |
Resumo: | Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved. |
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Evaluation of photodynamic therapy on fibroblast viability and cytokine productionEndodontic treatmentRoot canal irrigantsPhotodynamic therapyCytotoxicityCytokineBackground: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP, BrazilUniv Sao Paulo, Sch Dent, Discipline Endodont, Dept Restorat Dent, Sao Paulo, SP, BrazilUniv Sao Paulo, Phys Inst Sao Carlos, Opt Grp, Sao Carlos, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP, BrazilSao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP, BrazilFAPESP: 2011/23287-8FAPESP: 2012/12695-0FAPESP: 2012/06785-7Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Gomes-Filho, Joao Eduardo [UNESP]Sivieri-Araujo, Gustavo [UNESP]Sipert, Carla RenataSilva Santos, Ludmilla Mota da [UNESP]Azevedo Queiroz, India Olinta de [UNESP]Martins, Christine Men [UNESP]Carmo Maia, Nayara Kivilla do [UNESP]Angelo Cintra, Luciano Tavares [UNESP]Dezan-Junior, Eloi [UNESP]Bagnato, Vanderlei SalvadorChaves-Neto, Antonio Hernandes [UNESP]Penha de Oliveira, Sandra Helena [UNESP]2018-11-26T16:28:05Z2018-11-26T16:28:05Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-100application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2016.01.007Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016.1572-1000http://hdl.handle.net/11449/16133310.1016/j.pdpdt.2016.01.007WOS:000372376500015WOS000372376500015.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis And Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2024-09-19T18:31:51Zoai:repositorio.unesp.br:11449/161333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
title |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
spellingShingle |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production Gomes-Filho, Joao Eduardo [UNESP] Endodontic treatment Root canal irrigants Photodynamic therapy Cytotoxicity Cytokine |
title_short |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
title_full |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
title_fullStr |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
title_full_unstemmed |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
title_sort |
Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
author |
Gomes-Filho, Joao Eduardo [UNESP] |
author_facet |
Gomes-Filho, Joao Eduardo [UNESP] Sivieri-Araujo, Gustavo [UNESP] Sipert, Carla Renata Silva Santos, Ludmilla Mota da [UNESP] Azevedo Queiroz, India Olinta de [UNESP] Martins, Christine Men [UNESP] Carmo Maia, Nayara Kivilla do [UNESP] Angelo Cintra, Luciano Tavares [UNESP] Dezan-Junior, Eloi [UNESP] Bagnato, Vanderlei Salvador Chaves-Neto, Antonio Hernandes [UNESP] Penha de Oliveira, Sandra Helena [UNESP] |
author_role |
author |
author2 |
Sivieri-Araujo, Gustavo [UNESP] Sipert, Carla Renata Silva Santos, Ludmilla Mota da [UNESP] Azevedo Queiroz, India Olinta de [UNESP] Martins, Christine Men [UNESP] Carmo Maia, Nayara Kivilla do [UNESP] Angelo Cintra, Luciano Tavares [UNESP] Dezan-Junior, Eloi [UNESP] Bagnato, Vanderlei Salvador Chaves-Neto, Antonio Hernandes [UNESP] Penha de Oliveira, Sandra Helena [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gomes-Filho, Joao Eduardo [UNESP] Sivieri-Araujo, Gustavo [UNESP] Sipert, Carla Renata Silva Santos, Ludmilla Mota da [UNESP] Azevedo Queiroz, India Olinta de [UNESP] Martins, Christine Men [UNESP] Carmo Maia, Nayara Kivilla do [UNESP] Angelo Cintra, Luciano Tavares [UNESP] Dezan-Junior, Eloi [UNESP] Bagnato, Vanderlei Salvador Chaves-Neto, Antonio Hernandes [UNESP] Penha de Oliveira, Sandra Helena [UNESP] |
dc.subject.por.fl_str_mv |
Endodontic treatment Root canal irrigants Photodynamic therapy Cytotoxicity Cytokine |
topic |
Endodontic treatment Root canal irrigants Photodynamic therapy Cytotoxicity Cytokine |
description |
Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 2018-11-26T16:28:05Z 2018-11-26T16:28:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2016.01.007 Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016. 1572-1000 http://hdl.handle.net/11449/161333 10.1016/j.pdpdt.2016.01.007 WOS:000372376500015 WOS000372376500015.pdf |
url |
http://dx.doi.org/10.1016/j.pdpdt.2016.01.007 http://hdl.handle.net/11449/161333 |
identifier_str_mv |
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 13, p. 97-100, 2016. 1572-1000 10.1016/j.pdpdt.2016.01.007 WOS:000372376500015 WOS000372376500015.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis And Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
97-100 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546497863581696 |