Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201600520 http://hdl.handle.net/11449/178286 |
Resumo: | This clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells. |
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Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potentialBacteriaCytokinesEndodontic infectionEndotoxinRoot canalsThis clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells.Department of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual PaulistaDepartment of Odontology Endodontic Division UNITAU - Universidade de Taubat�Department of Restorative Dentistry Endodontic Division Piracicaba Dental School UNICAMP - Universidade Estadual de CampinasDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)UNITAU - Universidade de Taubat�Universidade Estadual de Campinas (UNICAMP)Cardoso, Fl�via Goulart Da Rosa [UNESP]Chung, Adriana [UNESP]Martinho, Frederico Canato [UNESP]Camargo, Carlos Henrique Ribeiro [UNESP]Carvalho, Claudio Ant�nio Talge [UNESP]Gomes, Brenda Paula Figueiredo De AlmeidaValera, Marcia Carneiro [UNESP]2018-12-11T17:29:38Z2018-12-11T17:29:38Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article412-418application/pdfhttp://dx.doi.org/10.1590/0103-6440201600520Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016.1806-47600103-6440http://hdl.handle.net/11449/17828610.1590/0103-6440201600520S0103-644020160004004122-s2.0-84988433786S0103-64402016000400412.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-11-17T06:08:38Zoai:repositorio.unesp.br:11449/178286Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-17T06:08:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
title |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
spellingShingle |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential Cardoso, Fl�via Goulart Da Rosa [UNESP] Bacteria Cytokines Endodontic infection Endotoxin Root canals |
title_short |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
title_full |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
title_fullStr |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
title_full_unstemmed |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
title_sort |
Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential |
author |
Cardoso, Fl�via Goulart Da Rosa [UNESP] |
author_facet |
Cardoso, Fl�via Goulart Da Rosa [UNESP] Chung, Adriana [UNESP] Martinho, Frederico Canato [UNESP] Camargo, Carlos Henrique Ribeiro [UNESP] Carvalho, Claudio Ant�nio Talge [UNESP] Gomes, Brenda Paula Figueiredo De Almeida Valera, Marcia Carneiro [UNESP] |
author_role |
author |
author2 |
Chung, Adriana [UNESP] Martinho, Frederico Canato [UNESP] Camargo, Carlos Henrique Ribeiro [UNESP] Carvalho, Claudio Ant�nio Talge [UNESP] Gomes, Brenda Paula Figueiredo De Almeida Valera, Marcia Carneiro [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNITAU - Universidade de Taubat� Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Cardoso, Fl�via Goulart Da Rosa [UNESP] Chung, Adriana [UNESP] Martinho, Frederico Canato [UNESP] Camargo, Carlos Henrique Ribeiro [UNESP] Carvalho, Claudio Ant�nio Talge [UNESP] Gomes, Brenda Paula Figueiredo De Almeida Valera, Marcia Carneiro [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Cytokines Endodontic infection Endotoxin Root canals |
topic |
Bacteria Cytokines Endodontic infection Endotoxin Root canals |
description |
This clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-12-11T17:29:38Z 2018-12-11T17:29:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201600520 Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016. 1806-4760 0103-6440 http://hdl.handle.net/11449/178286 10.1590/0103-6440201600520 S0103-64402016000400412 2-s2.0-84988433786 S0103-64402016000400412.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201600520 http://hdl.handle.net/11449/178286 |
identifier_str_mv |
Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016. 1806-4760 0103-6440 10.1590/0103-6440201600520 S0103-64402016000400412 2-s2.0-84988433786 S0103-64402016000400412.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
412-418 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964963620519936 |