Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential

Detalhes bibliográficos
Autor(a) principal: Cardoso, Fl�via Goulart Da Rosa [UNESP]
Data de Publicação: 2016
Outros Autores: Chung, Adriana [UNESP], Martinho, Frederico Canato [UNESP], Camargo, Carlos Henrique Ribeiro [UNESP], Carvalho, Claudio Ant�nio Talge [UNESP], Gomes, Brenda Paula Figueiredo De Almeida, Valera, Marcia Carneiro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-6440201600520
http://hdl.handle.net/11449/178286
Resumo: This clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells.
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spelling Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potentialBacteriaCytokinesEndodontic infectionEndotoxinRoot canalsThis clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells.Department of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual PaulistaDepartment of Odontology Endodontic Division UNITAU - Universidade de Taubat�Department of Restorative Dentistry Endodontic Division Piracicaba Dental School UNICAMP - Universidade Estadual de CampinasDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)UNITAU - Universidade de Taubat�Universidade Estadual de Campinas (UNICAMP)Cardoso, Fl�via Goulart Da Rosa [UNESP]Chung, Adriana [UNESP]Martinho, Frederico Canato [UNESP]Camargo, Carlos Henrique Ribeiro [UNESP]Carvalho, Claudio Ant�nio Talge [UNESP]Gomes, Brenda Paula Figueiredo De AlmeidaValera, Marcia Carneiro [UNESP]2018-12-11T17:29:38Z2018-12-11T17:29:38Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article412-418application/pdfhttp://dx.doi.org/10.1590/0103-6440201600520Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016.1806-47600103-6440http://hdl.handle.net/11449/17828610.1590/0103-6440201600520S0103-644020160004004122-s2.0-84988433786S0103-64402016000400412.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-11-17T06:08:38Zoai:repositorio.unesp.br:11449/178286Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-17T06:08:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
title Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
spellingShingle Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
Cardoso, Fl�via Goulart Da Rosa [UNESP]
Bacteria
Cytokines
Endodontic infection
Endotoxin
Root canals
title_short Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
title_full Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
title_fullStr Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
title_full_unstemmed Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
title_sort Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential
author Cardoso, Fl�via Goulart Da Rosa [UNESP]
author_facet Cardoso, Fl�via Goulart Da Rosa [UNESP]
Chung, Adriana [UNESP]
Martinho, Frederico Canato [UNESP]
Camargo, Carlos Henrique Ribeiro [UNESP]
Carvalho, Claudio Ant�nio Talge [UNESP]
Gomes, Brenda Paula Figueiredo De Almeida
Valera, Marcia Carneiro [UNESP]
author_role author
author2 Chung, Adriana [UNESP]
Martinho, Frederico Canato [UNESP]
Camargo, Carlos Henrique Ribeiro [UNESP]
Carvalho, Claudio Ant�nio Talge [UNESP]
Gomes, Brenda Paula Figueiredo De Almeida
Valera, Marcia Carneiro [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNITAU - Universidade de Taubat�
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Cardoso, Fl�via Goulart Da Rosa [UNESP]
Chung, Adriana [UNESP]
Martinho, Frederico Canato [UNESP]
Camargo, Carlos Henrique Ribeiro [UNESP]
Carvalho, Claudio Ant�nio Talge [UNESP]
Gomes, Brenda Paula Figueiredo De Almeida
Valera, Marcia Carneiro [UNESP]
dc.subject.por.fl_str_mv Bacteria
Cytokines
Endodontic infection
Endotoxin
Root canals
topic Bacteria
Cytokines
Endodontic infection
Endotoxin
Root canals
description This clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-01
2018-12-11T17:29:38Z
2018-12-11T17:29:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-6440201600520
Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016.
1806-4760
0103-6440
http://hdl.handle.net/11449/178286
10.1590/0103-6440201600520
S0103-64402016000400412
2-s2.0-84988433786
S0103-64402016000400412.pdf
url http://dx.doi.org/10.1590/0103-6440201600520
http://hdl.handle.net/11449/178286
identifier_str_mv Brazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016.
1806-4760
0103-6440
10.1590/0103-6440201600520
S0103-64402016000400412
2-s2.0-84988433786
S0103-64402016000400412.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Dental Journal
0,476
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 412-418
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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