Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201902239 http://hdl.handle.net/11449/189021 |
Resumo: | This clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p<0.05). Positive correlations were found between bacteria species and presence of different clinical features (p<0.05). After grouping the bacteria species into bacterial complexes, positive associations were found between green, orange and red complexes with presence of sinus tract (p<0.05). This clinical study revealed that larger root canals hold higher levels of culturable bacteria in PEI. Thus, the interaction of different virulent bacteria species in complexes seems to play an important role in the development of clinical features. |
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Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infectionBacteriaEndodontic infectionEndotoxinThis clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p<0.05). Positive correlations were found between bacteria species and presence of different clinical features (p<0.05). After grouping the bacteria species into bacterial complexes, positive associations were found between green, orange and red complexes with presence of sinus tract (p<0.05). This clinical study revealed that larger root canals hold higher levels of culturable bacteria in PEI. Thus, the interaction of different virulent bacteria species in complexes seems to play an important role in the development of clinical features.Department of Restorative Dentistry Institute of Science and Technology UNESP - Univ Estadual Paulista, São José dos CamposDepartment of Dentistry Endodontic Division University of Taubaté – UNITAUDepartment of Advanced Oral Science & Therapeutics Endodontic Division University of Maryland School of DentistryDepartment of Semiology UFPel - Universidade Federal de Pelotas School of DentistryDepartment of Radiology Institute of Science and Technology UNESP - Univ Estadual PaulistaDepartment of Restorative Dentistry Institute of Science and Technology UNESP - Univ Estadual Paulista, São José dos CamposDepartment of Radiology Institute of Science and Technology UNESP - Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)University of Taubaté – UNITAUSchool of DentistryUniversidade Federal de Pernambuco (UFPE)Cardoso, Flávia Goulart da Rosa [UNESP]Martinho, Frederico Canato [UNESP]Ferreira, Nádia de Souza [UNESP]do Prado, Renata Falchete [UNESP]Manhães-Júnior, Luiz Roberto Coutinho [UNESP]Rocco, Marco Antônio [UNESP]Valera, Marcia Carneiro [UNESP]2019-10-06T16:27:13Z2019-10-06T16:27:13Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article117-122application/pdfhttp://dx.doi.org/10.1590/0103-6440201902239Brazilian Dental Journal, v. 30, n. 2, p. 117-122, 2019.1806-47600103-6440http://hdl.handle.net/11449/18902110.1590/0103-6440201902239S0103-644020190002001172-s2.0-85064722622S0103-64402019000200117.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-12-19T06:24:54Zoai:repositorio.unesp.br:11449/189021Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-19T06:24:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
title |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
spellingShingle |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection Cardoso, Flávia Goulart da Rosa [UNESP] Bacteria Endodontic infection Endotoxin |
title_short |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
title_full |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
title_fullStr |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
title_full_unstemmed |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
title_sort |
Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection |
author |
Cardoso, Flávia Goulart da Rosa [UNESP] |
author_facet |
Cardoso, Flávia Goulart da Rosa [UNESP] Martinho, Frederico Canato [UNESP] Ferreira, Nádia de Souza [UNESP] do Prado, Renata Falchete [UNESP] Manhães-Júnior, Luiz Roberto Coutinho [UNESP] Rocco, Marco Antônio [UNESP] Valera, Marcia Carneiro [UNESP] |
author_role |
author |
author2 |
Martinho, Frederico Canato [UNESP] Ferreira, Nádia de Souza [UNESP] do Prado, Renata Falchete [UNESP] Manhães-Júnior, Luiz Roberto Coutinho [UNESP] Rocco, Marco Antônio [UNESP] Valera, Marcia Carneiro [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Taubaté – UNITAU School of Dentistry Universidade Federal de Pernambuco (UFPE) |
dc.contributor.author.fl_str_mv |
Cardoso, Flávia Goulart da Rosa [UNESP] Martinho, Frederico Canato [UNESP] Ferreira, Nádia de Souza [UNESP] do Prado, Renata Falchete [UNESP] Manhães-Júnior, Luiz Roberto Coutinho [UNESP] Rocco, Marco Antônio [UNESP] Valera, Marcia Carneiro [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Endodontic infection Endotoxin |
topic |
Bacteria Endodontic infection Endotoxin |
description |
This clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p<0.05). Positive correlations were found between bacteria species and presence of different clinical features (p<0.05). After grouping the bacteria species into bacterial complexes, positive associations were found between green, orange and red complexes with presence of sinus tract (p<0.05). This clinical study revealed that larger root canals hold higher levels of culturable bacteria in PEI. Thus, the interaction of different virulent bacteria species in complexes seems to play an important role in the development of clinical features. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:27:13Z 2019-10-06T16:27:13Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201902239 Brazilian Dental Journal, v. 30, n. 2, p. 117-122, 2019. 1806-4760 0103-6440 http://hdl.handle.net/11449/189021 10.1590/0103-6440201902239 S0103-64402019000200117 2-s2.0-85064722622 S0103-64402019000200117.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201902239 http://hdl.handle.net/11449/189021 |
identifier_str_mv |
Brazilian Dental Journal, v. 30, n. 2, p. 117-122, 2019. 1806-4760 0103-6440 10.1590/0103-6440201902239 S0103-64402019000200117 2-s2.0-85064722622 S0103-64402019000200117.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
117-122 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965339743682560 |