The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis

Detalhes bibliográficos
Autor(a) principal: Pereira, Jaqueline A.
Data de Publicação: 2021
Outros Autores: Milani, Diogo [UNESP], Ferretti, Ana Beatriz S. M. [UNESP], Bardella, Vanessa B. [UNESP], Cabral-de-Mello, Diogo C. [UNESP], Lopes, Denilce M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00412-021-00764-x
http://hdl.handle.net/11449/222927
Resumo: Satellite DNAs (satDNAs) and transposable elements (TEs) are among the main components of constitutive heterochromatin (c-heterochromatin) and are related to their functionality, dynamics, and evolution. A peculiar case regarding the quantity and distribution of c-heterochromatin is observed in the genus of bees, Melipona, with species having a low amount of heterochromatin and species with high amount occupying almost all chromosomes. By combining low-pass genome sequencing and chromosomal analysis, we characterized the satDNAs and TEs of Melipona quadrifasciata (low c-heterochromatin) and Melipona scutellaris (high low c-heterochromatin) to understand c-heterochromatin composition and evolution. We identified 15 satDNA families and 20 TEs for both species. Significant variations in the repeat landscapes were observed between the species. In M. quadrifasciata, the repetitive fraction corresponded to only 3.78% of the genome library studied, whereas in M. scutellaris, it represented 54.95%. Massive quantitative and qualitative changes contributed to the differential amplification of c-heterochromatin, mainly due to the amplification of exclusive repetitions in M. scutellaris, as the satDNA MscuSat01-195 and the TE LTR/Gypsy_1 that represent 38.20 and 14.4% of its genome, respectively. The amplification of these two repeats is evident at the chromosomal level, with observation of their occurrence on most c-heterochromatin. Moreover, we detected repeats shared between species, revealing that they experienced mainly quantitative variations and varied in the organization on chromosomes and evolutionary patterns. Together, our data allow the discussion of patterns of evolution of repetitive DNAs and c-heterochromatin that occurred in a short period of time, after separation of the Michmelia and Melipona subgenera.
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spelling The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysisFluorescence in situ hybridizationRepeatomeSatellite DNAsStingless beesTransposable elementsSatellite DNAs (satDNAs) and transposable elements (TEs) are among the main components of constitutive heterochromatin (c-heterochromatin) and are related to their functionality, dynamics, and evolution. A peculiar case regarding the quantity and distribution of c-heterochromatin is observed in the genus of bees, Melipona, with species having a low amount of heterochromatin and species with high amount occupying almost all chromosomes. By combining low-pass genome sequencing and chromosomal analysis, we characterized the satDNAs and TEs of Melipona quadrifasciata (low c-heterochromatin) and Melipona scutellaris (high low c-heterochromatin) to understand c-heterochromatin composition and evolution. We identified 15 satDNA families and 20 TEs for both species. Significant variations in the repeat landscapes were observed between the species. In M. quadrifasciata, the repetitive fraction corresponded to only 3.78% of the genome library studied, whereas in M. scutellaris, it represented 54.95%. Massive quantitative and qualitative changes contributed to the differential amplification of c-heterochromatin, mainly due to the amplification of exclusive repetitions in M. scutellaris, as the satDNA MscuSat01-195 and the TE LTR/Gypsy_1 that represent 38.20 and 14.4% of its genome, respectively. The amplification of these two repeats is evident at the chromosomal level, with observation of their occurrence on most c-heterochromatin. Moreover, we detected repeats shared between species, revealing that they experienced mainly quantitative variations and varied in the organization on chromosomes and evolutionary patterns. Together, our data allow the discussion of patterns of evolution of repetitive DNAs and c-heterochromatin that occurred in a short period of time, after separation of the Michmelia and Melipona subgenera.Laboratório de Citogenética de Insetos Departamento de Biologia Geral Universidade Federal de Viçosa, Campus Viçosa, Av. P.H. Rolfs s/nDepartamento de Biologia Geral E Aplicada Instituto de Biociências/IB UNESP–Universidade Estadual PaulistaDepartamento de Biologia Geral E Aplicada Instituto de Biociências/IB UNESP–Universidade Estadual PaulistaUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (UNESP)Pereira, Jaqueline A.Milani, Diogo [UNESP]Ferretti, Ana Beatriz S. M. [UNESP]Bardella, Vanessa B. [UNESP]Cabral-de-Mello, Diogo C. [UNESP]Lopes, Denilce M.2022-04-28T19:47:38Z2022-04-28T19:47:38Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00412-021-00764-xChromosoma.1432-08860009-5915http://hdl.handle.net/11449/22292710.1007/s00412-021-00764-x2-s2.0-85119961686Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChromosomainfo:eu-repo/semantics/openAccess2022-04-28T19:47:38Zoai:repositorio.unesp.br:11449/222927Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:47:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
title The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
spellingShingle The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
Pereira, Jaqueline A.
Fluorescence in situ hybridization
Repeatome
Satellite DNAs
Stingless bees
Transposable elements
title_short The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
title_full The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
title_fullStr The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
title_full_unstemmed The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
title_sort The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis
author Pereira, Jaqueline A.
author_facet Pereira, Jaqueline A.
Milani, Diogo [UNESP]
Ferretti, Ana Beatriz S. M. [UNESP]
Bardella, Vanessa B. [UNESP]
Cabral-de-Mello, Diogo C. [UNESP]
Lopes, Denilce M.
author_role author
author2 Milani, Diogo [UNESP]
Ferretti, Ana Beatriz S. M. [UNESP]
Bardella, Vanessa B. [UNESP]
Cabral-de-Mello, Diogo C. [UNESP]
Lopes, Denilce M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pereira, Jaqueline A.
Milani, Diogo [UNESP]
Ferretti, Ana Beatriz S. M. [UNESP]
Bardella, Vanessa B. [UNESP]
Cabral-de-Mello, Diogo C. [UNESP]
Lopes, Denilce M.
dc.subject.por.fl_str_mv Fluorescence in situ hybridization
Repeatome
Satellite DNAs
Stingless bees
Transposable elements
topic Fluorescence in situ hybridization
Repeatome
Satellite DNAs
Stingless bees
Transposable elements
description Satellite DNAs (satDNAs) and transposable elements (TEs) are among the main components of constitutive heterochromatin (c-heterochromatin) and are related to their functionality, dynamics, and evolution. A peculiar case regarding the quantity and distribution of c-heterochromatin is observed in the genus of bees, Melipona, with species having a low amount of heterochromatin and species with high amount occupying almost all chromosomes. By combining low-pass genome sequencing and chromosomal analysis, we characterized the satDNAs and TEs of Melipona quadrifasciata (low c-heterochromatin) and Melipona scutellaris (high low c-heterochromatin) to understand c-heterochromatin composition and evolution. We identified 15 satDNA families and 20 TEs for both species. Significant variations in the repeat landscapes were observed between the species. In M. quadrifasciata, the repetitive fraction corresponded to only 3.78% of the genome library studied, whereas in M. scutellaris, it represented 54.95%. Massive quantitative and qualitative changes contributed to the differential amplification of c-heterochromatin, mainly due to the amplification of exclusive repetitions in M. scutellaris, as the satDNA MscuSat01-195 and the TE LTR/Gypsy_1 that represent 38.20 and 14.4% of its genome, respectively. The amplification of these two repeats is evident at the chromosomal level, with observation of their occurrence on most c-heterochromatin. Moreover, we detected repeats shared between species, revealing that they experienced mainly quantitative variations and varied in the organization on chromosomes and evolutionary patterns. Together, our data allow the discussion of patterns of evolution of repetitive DNAs and c-heterochromatin that occurred in a short period of time, after separation of the Michmelia and Melipona subgenera.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-28T19:47:38Z
2022-04-28T19:47:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00412-021-00764-x
Chromosoma.
1432-0886
0009-5915
http://hdl.handle.net/11449/222927
10.1007/s00412-021-00764-x
2-s2.0-85119961686
url http://dx.doi.org/10.1007/s00412-021-00764-x
http://hdl.handle.net/11449/222927
identifier_str_mv Chromosoma.
1432-0886
0009-5915
10.1007/s00412-021-00764-x
2-s2.0-85119961686
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chromosoma
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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