Identification of ellagic acid derivatives in methanolic extracts from Qualea species

• Autor(a) principal: Nasser, Ana L. M. [UNESP]
• Data de Publicação: 2008
• Outros Autores: Carli, Camila B. A. [UNESP], Rodrigues, Clenilson M. [UNESP], Maia, Danielle C. G. [UNESP], Carlos, Iracilda Zeppone [UNESP], Eberlin, Marcos N., Hiruma-Lima, Clélia A. [UNESP], Vilegas, Wagner [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.znaturforsch.com/s63c/s63c0789.pdf; http://hdl.handle.net/11449/70831
• topic: Ellagic acid derivatives; Liquid chromatography; Qualea; ellagic acid; lipopolysaccharide; methanol; nitric oxide; plant extract; thioglycolic acid derivative; tumor necrosis factor alpha; angiosperm; animal; cell survival; chemistry; cytology; drug effect; high performance liquid chromatography; isolation and purification; macrophage; metabolism; mouse; nuclear magnetic resonance spectroscopy; physiology; Angiosperms; Animals; Cell Survival; Chromatography, High Pressure Liquid; Ellagic Acid; Lipopolysaccharides; Macrophages; Magnetic Resonance Spectroscopy; Methanol; Mice; Nitric Oxide; Plant Extracts; Thioglycolates; Tumor Necrosis Factor-alpha; Murinae; Qualea grandiflora; Qualea multiflora; Vochysiaceae

The methanolic extract from the barks of the medicinal plant Qualea parviflora (Vochysiaceae) was fractionated by column chromatography over silica gel followed by gel permeation over Sephadex LH-20 to give 3,3′-di-O- methylellagic acid-4-O-β-D-glucopyranoside (1), 3-O-methylellagic acid-4′-O-α-L-rhamnopyranoside (2), 3,3′,4-tri-O-methylellagic acid-4′-O-β-D-glucopyranoside (3), and 3,3′-di-O-methylellagic acid (4), together with triterpenes and saponins. We also performed comparative analyses among this species and Q. grandiflora and Q. multiflora using high-pressure liquid chromatography. The biological assays showed that, when compared to the standard ellagic acid, compounds 1-4 are less cytotoxic but have a lower capacity of stimulating murine peritoneal macrophages to release nitric oxide and tumoural-α necrose factor. © 2008 Verlag der Zeitschrift für Naturforschung.