Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles

Detalhes bibliográficos
Autor(a) principal: Silva, A. A. [UNESP]
Data de Publicação: 2006
Outros Autores: Formariz, T. P. [UNESP], Scarpa, M. V. [UNESP], Oliveira, Anselmo Gomes de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/371
http://hdl.handle.net/11449/69345
Resumo: A simple, rapid, selective and specific high performance liquid chromatographic (HPLC) method for quantitative analysis of the triamcinolone in polylactide-co-glycolide acid (PLGA) microparticles was developed. The chromatographic parameters were reversed-phase C18 column, 250mm x 4.6mm, with particle size 5 μm. The column oven was thermostated at 35°C ± 2°C. The mobile phase was methanol/water 45:55 (v/v) and elution was isocratic at a flow-rate of 1 mL.mL-1. The determinations were performed using a UV-Vis detector at 239 nm. The injected sample volume was 10 μL. The standard curve was linear (r2 > 0.999) in the concentration range 100-2500 ng.mL-1. The method showed adequate precision, with a relative standard deviation (RSD) was smaller than 3%. The accuracy was analyzed by adding a standard drug and good recovery values were obtained for all drug concentrations used. The method showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for quantitation of triamcinolone in PLGA microparticles.
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spelling Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticlesAnalytical method validationHPLC analytical methodPLGA microparticlesTriamcinoloneglycolic acid derivativemethanoltriamcinolonewaterbiodegradabilityconcentration (parameters)diagnostic accuracydiagnostic valueelutionflow ratehigh performance liquid chromatographyinjectionparticle sizequantitative analysissamplestandardthermostabilityvalidation studyA simple, rapid, selective and specific high performance liquid chromatographic (HPLC) method for quantitative analysis of the triamcinolone in polylactide-co-glycolide acid (PLGA) microparticles was developed. The chromatographic parameters were reversed-phase C18 column, 250mm x 4.6mm, with particle size 5 μm. The column oven was thermostated at 35°C ± 2°C. The mobile phase was methanol/water 45:55 (v/v) and elution was isocratic at a flow-rate of 1 mL.mL-1. The determinations were performed using a UV-Vis detector at 239 nm. The injected sample volume was 10 μL. The standard curve was linear (r2 > 0.999) in the concentration range 100-2500 ng.mL-1. The method showed adequate precision, with a relative standard deviation (RSD) was smaller than 3%. The accuracy was analyzed by adding a standard drug and good recovery values were obtained for all drug concentrations used. The method showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for quantitation of triamcinolone in PLGA microparticles.Departamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SPDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jau, km. 01, CEP: 14801-902 - Araraquara - SPDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SPDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jau, km. 01, CEP: 14801-902 - Araraquara - SPUniversidade Estadual Paulista (Unesp)Silva, A. A. [UNESP]Formariz, T. P. [UNESP]Scarpa, M. V. [UNESP]Oliveira, Anselmo Gomes de [UNESP]2014-05-27T11:22:05Z2014-05-27T11:22:05Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-126application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/371Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 2, p. 119-126, 2006.1808-4532http://hdl.handle.net/11449/693452-s2.0-353489620722-s2.0-35348962072.pdf9114495952533044Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:46:24Zoai:repositorio.unesp.br:11449/69345Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:38:44.721520Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
title Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
spellingShingle Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
Silva, A. A. [UNESP]
Analytical method validation
HPLC analytical method
PLGA microparticles
Triamcinolone
glycolic acid derivative
methanol
triamcinolone
water
biodegradability
concentration (parameters)
diagnostic accuracy
diagnostic value
elution
flow rate
high performance liquid chromatography
injection
particle size
quantitative analysis
sample
standard
thermostability
validation study
title_short Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
title_full Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
title_fullStr Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
title_full_unstemmed Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
title_sort Development and validation of HPLC method for quantitative analysis of triamcinolone in biodegradable microparticles
author Silva, A. A. [UNESP]
author_facet Silva, A. A. [UNESP]
Formariz, T. P. [UNESP]
Scarpa, M. V. [UNESP]
Oliveira, Anselmo Gomes de [UNESP]
author_role author
author2 Formariz, T. P. [UNESP]
Scarpa, M. V. [UNESP]
Oliveira, Anselmo Gomes de [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, A. A. [UNESP]
Formariz, T. P. [UNESP]
Scarpa, M. V. [UNESP]
Oliveira, Anselmo Gomes de [UNESP]
dc.subject.por.fl_str_mv Analytical method validation
HPLC analytical method
PLGA microparticles
Triamcinolone
glycolic acid derivative
methanol
triamcinolone
water
biodegradability
concentration (parameters)
diagnostic accuracy
diagnostic value
elution
flow rate
high performance liquid chromatography
injection
particle size
quantitative analysis
sample
standard
thermostability
validation study
topic Analytical method validation
HPLC analytical method
PLGA microparticles
Triamcinolone
glycolic acid derivative
methanol
triamcinolone
water
biodegradability
concentration (parameters)
diagnostic accuracy
diagnostic value
elution
flow rate
high performance liquid chromatography
injection
particle size
quantitative analysis
sample
standard
thermostability
validation study
description A simple, rapid, selective and specific high performance liquid chromatographic (HPLC) method for quantitative analysis of the triamcinolone in polylactide-co-glycolide acid (PLGA) microparticles was developed. The chromatographic parameters were reversed-phase C18 column, 250mm x 4.6mm, with particle size 5 μm. The column oven was thermostated at 35°C ± 2°C. The mobile phase was methanol/water 45:55 (v/v) and elution was isocratic at a flow-rate of 1 mL.mL-1. The determinations were performed using a UV-Vis detector at 239 nm. The injected sample volume was 10 μL. The standard curve was linear (r2 > 0.999) in the concentration range 100-2500 ng.mL-1. The method showed adequate precision, with a relative standard deviation (RSD) was smaller than 3%. The accuracy was analyzed by adding a standard drug and good recovery values were obtained for all drug concentrations used. The method showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for quantitation of triamcinolone in PLGA microparticles.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
2014-05-27T11:22:05Z
2014-05-27T11:22:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/371
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 2, p. 119-126, 2006.
1808-4532
http://hdl.handle.net/11449/69345
2-s2.0-35348962072
2-s2.0-35348962072.pdf
9114495952533044
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/371
http://hdl.handle.net/11449/69345
identifier_str_mv Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 2, p. 119-126, 2006.
1808-4532
2-s2.0-35348962072
2-s2.0-35348962072.pdf
9114495952533044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 119-126
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129446091161600