Alternative oxidase confers nutritional limitation on Drosophila development
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jez.2274 http://hdl.handle.net/11449/210048 |
Resumo: | The mitochondrial alternative oxidase, AOX, present in most eukaryotes apart from vertebrates and insects, catalyzes the direct oxidation of ubiquinol by oxygen, by-passing the terminal proton-motive steps of the respiratory chain. Its physiological role is not fully understood, but it is proposed to buffer stresses in the respiratory chain similar to those encountered in mitochondrial diseases in humans. Previously, we found that the ubiquitous expression of AOX from Ciona intestinalis in Drosophila perturbs the development of flies cultured under low-nutrient conditions (media containing only glucose and yeast). Here we tested the effects of a wide range of nutritional supplements on Drosophila development, to gain insight into the physiological mechanism underlying this developmental failure. On low-nutrient medium, larvae contained decreased amounts of triglycerides, lactate, and pyruvate, irrespective of AOX expression. Complex food supplements, including treacle (molasses), restored normal development to AOX-expressing flies, but many individual additives did not. Inhibition of AOX by treacle extract was excluded as a mechanism, since the supplement did not alter the enzymatic activity of AOX in vitro. Furthermore, antibiotics did not influence the organismal phenotype, indicating that commensal microbes were not involved. Fractionation of treacle identified a water-soluble fraction with low solubility in ethanol, rich in lactate and tricarboxylic acid cycle intermediates, which contained the critical activity. We propose that the partial activation of AOX during metamorphosis impairs the efficient use of stored metabolites, resulting in developmental failure. |
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Alternative oxidase confers nutritional limitation on Drosophila developmentAOXcataplerosismitochondrianutritionTCA cycleThe mitochondrial alternative oxidase, AOX, present in most eukaryotes apart from vertebrates and insects, catalyzes the direct oxidation of ubiquinol by oxygen, by-passing the terminal proton-motive steps of the respiratory chain. Its physiological role is not fully understood, but it is proposed to buffer stresses in the respiratory chain similar to those encountered in mitochondrial diseases in humans. Previously, we found that the ubiquitous expression of AOX from Ciona intestinalis in Drosophila perturbs the development of flies cultured under low-nutrient conditions (media containing only glucose and yeast). Here we tested the effects of a wide range of nutritional supplements on Drosophila development, to gain insight into the physiological mechanism underlying this developmental failure. On low-nutrient medium, larvae contained decreased amounts of triglycerides, lactate, and pyruvate, irrespective of AOX expression. Complex food supplements, including treacle (molasses), restored normal development to AOX-expressing flies, but many individual additives did not. Inhibition of AOX by treacle extract was excluded as a mechanism, since the supplement did not alter the enzymatic activity of AOX in vitro. Furthermore, antibiotics did not influence the organismal phenotype, indicating that commensal microbes were not involved. Fractionation of treacle identified a water-soluble fraction with low solubility in ethanol, rich in lactate and tricarboxylic acid cycle intermediates, which contained the critical activity. We propose that the partial activation of AOX during metamorphosis impairs the efficient use of stored metabolites, resulting in developmental failure.FP7 Ideas: European Research CouncilTampere University Hospital Medical Research FundSuomen AkatemiaSigrid Juselius FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Tampere Univ, Fac Med & Hlth Technol, FI-33014 Tampere, FinlandTampere Univ, Tampere Univ Hosp, Tampere, FinlandUniv Turku, Turku Ctr Biotechnol, Turku, FinlandAbo Akad Univ, Turku, FinlandUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilUniv Helsinki, Inst Biotechnol, Helsinki, FinlandUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilFP7 Ideas: European Research Council: 232738Suomen Akatemia: 272376Suomen Akatemia: 283157FAPESP: 2014/02253-6CNPq: 424562/2018-9Wiley-BlackwellTampere UnivUniv TurkuAbo Akad UnivUniversidade Estadual Paulista (Unesp)Univ HelsinkiSaari, SinaKemppainen, EskoTuomela, TeaOliveira, Marcos T. [UNESP]Dufour, EricJacobs, Howard T.2021-06-25T12:38:04Z2021-06-25T12:38:04Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article341-356http://dx.doi.org/10.1002/jez.2274Journal Of Experimental Zoology Part A-ecological And Integrative Physiology. Hoboken: Wiley, v. 331, n. 6, p. 341-356, 2019.2471-5638http://hdl.handle.net/11449/21004810.1002/jez.2274WOS:000618827300003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Experimental Zoology Part A-ecological And Integrative Physiologyinfo:eu-repo/semantics/openAccess2024-06-07T15:31:59Zoai:repositorio.unesp.br:11449/210048Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:07:12.023315Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alternative oxidase confers nutritional limitation on Drosophila development |
title |
Alternative oxidase confers nutritional limitation on Drosophila development |
spellingShingle |
Alternative oxidase confers nutritional limitation on Drosophila development Saari, Sina AOX cataplerosis mitochondria nutrition TCA cycle |
title_short |
Alternative oxidase confers nutritional limitation on Drosophila development |
title_full |
Alternative oxidase confers nutritional limitation on Drosophila development |
title_fullStr |
Alternative oxidase confers nutritional limitation on Drosophila development |
title_full_unstemmed |
Alternative oxidase confers nutritional limitation on Drosophila development |
title_sort |
Alternative oxidase confers nutritional limitation on Drosophila development |
author |
Saari, Sina |
author_facet |
Saari, Sina Kemppainen, Esko Tuomela, Tea Oliveira, Marcos T. [UNESP] Dufour, Eric Jacobs, Howard T. |
author_role |
author |
author2 |
Kemppainen, Esko Tuomela, Tea Oliveira, Marcos T. [UNESP] Dufour, Eric Jacobs, Howard T. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Tampere Univ Univ Turku Abo Akad Univ Universidade Estadual Paulista (Unesp) Univ Helsinki |
dc.contributor.author.fl_str_mv |
Saari, Sina Kemppainen, Esko Tuomela, Tea Oliveira, Marcos T. [UNESP] Dufour, Eric Jacobs, Howard T. |
dc.subject.por.fl_str_mv |
AOX cataplerosis mitochondria nutrition TCA cycle |
topic |
AOX cataplerosis mitochondria nutrition TCA cycle |
description |
The mitochondrial alternative oxidase, AOX, present in most eukaryotes apart from vertebrates and insects, catalyzes the direct oxidation of ubiquinol by oxygen, by-passing the terminal proton-motive steps of the respiratory chain. Its physiological role is not fully understood, but it is proposed to buffer stresses in the respiratory chain similar to those encountered in mitochondrial diseases in humans. Previously, we found that the ubiquitous expression of AOX from Ciona intestinalis in Drosophila perturbs the development of flies cultured under low-nutrient conditions (media containing only glucose and yeast). Here we tested the effects of a wide range of nutritional supplements on Drosophila development, to gain insight into the physiological mechanism underlying this developmental failure. On low-nutrient medium, larvae contained decreased amounts of triglycerides, lactate, and pyruvate, irrespective of AOX expression. Complex food supplements, including treacle (molasses), restored normal development to AOX-expressing flies, but many individual additives did not. Inhibition of AOX by treacle extract was excluded as a mechanism, since the supplement did not alter the enzymatic activity of AOX in vitro. Furthermore, antibiotics did not influence the organismal phenotype, indicating that commensal microbes were not involved. Fractionation of treacle identified a water-soluble fraction with low solubility in ethanol, rich in lactate and tricarboxylic acid cycle intermediates, which contained the critical activity. We propose that the partial activation of AOX during metamorphosis impairs the efficient use of stored metabolites, resulting in developmental failure. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01 2021-06-25T12:38:04Z 2021-06-25T12:38:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jez.2274 Journal Of Experimental Zoology Part A-ecological And Integrative Physiology. Hoboken: Wiley, v. 331, n. 6, p. 341-356, 2019. 2471-5638 http://hdl.handle.net/11449/210048 10.1002/jez.2274 WOS:000618827300003 |
url |
http://dx.doi.org/10.1002/jez.2274 http://hdl.handle.net/11449/210048 |
identifier_str_mv |
Journal Of Experimental Zoology Part A-ecological And Integrative Physiology. Hoboken: Wiley, v. 331, n. 6, p. 341-356, 2019. 2471-5638 10.1002/jez.2274 WOS:000618827300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Experimental Zoology Part A-ecological And Integrative Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
341-356 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128898102198272 |