Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis

Detalhes bibliográficos
Autor(a) principal: El Achkar, Vivian Narana Ribeiro [UNESP]
Data de Publicação: 2020
Outros Autores: Duarte, Andressa, Carlos, Román, León, Jorge Esquiche, Ribeiro-Silva, Alfredo, Pignatari, Shirley Shizue Nagata, Kaminagakura, Estela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.amjoto.2019.102321
http://hdl.handle.net/11449/198226
Resumo: Objective: To characterize inflammatory cells in Recurrent Respiratory Papillomatosis (RRP) and to correlate it with severity using the Derkay laryngoscopic scale. Materials and methods: The data and biopsies from 36 patients with Juvenile (JRRP) and 56 patients with Adult (ARRP) were collected and analyzed under light microscopy. The patients were separated into groups according to the Derkay index: ≥20 for the most severe and < 20 for the less severe cases. Immunohistochemical analysis using CD3, CD4, CD8, CD15, CD20, CD68, FoxP3 and MUM-1 antibodies was performed, and the inflammatory cells were quantified. All the clinicopathological characteristics and the results of the immunohistochemical analysis were compared among the groups proposed using the Chi-Square test and correlated through the Spearman correlation test. Results: The ARRP showed significantly higher quantities of CD3+, CD8+ and MUM1+ cells (p < .05) than the JRRP samples. The presence of CD15+ cells showed positive correlation with the Derkay index (p < .05), while the MUM-1+ cells showed an inverse correlation (p = .01). Conclusion: There are differences between the inflammatory cells population in the juvenile and adult groups and it can be related to disease severity.
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spelling Relationship between inflammation and the severity of Recurrent Respiratory PapillomatosisHuman papillomavirusInflammatory cellsLaryngeal papillomatosisSquamous cell papillomatosisObjective: To characterize inflammatory cells in Recurrent Respiratory Papillomatosis (RRP) and to correlate it with severity using the Derkay laryngoscopic scale. Materials and methods: The data and biopsies from 36 patients with Juvenile (JRRP) and 56 patients with Adult (ARRP) were collected and analyzed under light microscopy. The patients were separated into groups according to the Derkay index: ≥20 for the most severe and < 20 for the less severe cases. Immunohistochemical analysis using CD3, CD4, CD8, CD15, CD20, CD68, FoxP3 and MUM-1 antibodies was performed, and the inflammatory cells were quantified. All the clinicopathological characteristics and the results of the immunohistochemical analysis were compared among the groups proposed using the Chi-Square test and correlated through the Spearman correlation test. Results: The ARRP showed significantly higher quantities of CD3+, CD8+ and MUM1+ cells (p < .05) than the JRRP samples. The presence of CD15+ cells showed positive correlation with the Derkay index (p < .05), while the MUM-1+ cells showed an inverse correlation (p = .01). Conclusion: There are differences between the inflammatory cells population in the juvenile and adult groups and it can be related to disease severity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESPDepartment of Pathology Ribeirão Preto School of Medicine University of São PauloPathology Department Hospital Herrera-LlerandiOral Pathology Department of Stomatology Public Oral Health and Forensic Dentistry School of Dentistry of Ribeirão Preto University of São PauloDepartment of Pathology and Legal Medicine Ribeirão Preto School of Medicine University of São PauloDepartment of Otorhinolaryngology and Head and Neck Surgery São Paulo Federal UniversityDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESPFAPESP: 16/24019-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Hospital Herrera-LlerandiSão Paulo Federal UniversityEl Achkar, Vivian Narana Ribeiro [UNESP]Duarte, AndressaCarlos, RománLeón, Jorge EsquicheRibeiro-Silva, AlfredoPignatari, Shirley Shizue NagataKaminagakura, Estela [UNESP]2020-12-12T01:07:01Z2020-12-12T01:07:01Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.amjoto.2019.102321American Journal of Otolaryngology - Head and Neck Medicine and Surgery, v. 41, n. 2, 2020.1532-818X0196-0709http://hdl.handle.net/11449/19822610.1016/j.amjoto.2019.1023212-s2.0-85075903383Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican Journal of Otolaryngology - Head and Neck Medicine and Surgeryinfo:eu-repo/semantics/openAccess2021-10-23T10:02:23Zoai:repositorio.unesp.br:11449/198226Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:01:50.655569Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
title Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
spellingShingle Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
El Achkar, Vivian Narana Ribeiro [UNESP]
Human papillomavirus
Inflammatory cells
Laryngeal papillomatosis
Squamous cell papillomatosis
title_short Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
title_full Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
title_fullStr Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
title_full_unstemmed Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
title_sort Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis
author El Achkar, Vivian Narana Ribeiro [UNESP]
author_facet El Achkar, Vivian Narana Ribeiro [UNESP]
Duarte, Andressa
Carlos, Román
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Pignatari, Shirley Shizue Nagata
Kaminagakura, Estela [UNESP]
author_role author
author2 Duarte, Andressa
Carlos, Román
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Pignatari, Shirley Shizue Nagata
Kaminagakura, Estela [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Hospital Herrera-Llerandi
São Paulo Federal University
dc.contributor.author.fl_str_mv El Achkar, Vivian Narana Ribeiro [UNESP]
Duarte, Andressa
Carlos, Román
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Pignatari, Shirley Shizue Nagata
Kaminagakura, Estela [UNESP]
dc.subject.por.fl_str_mv Human papillomavirus
Inflammatory cells
Laryngeal papillomatosis
Squamous cell papillomatosis
topic Human papillomavirus
Inflammatory cells
Laryngeal papillomatosis
Squamous cell papillomatosis
description Objective: To characterize inflammatory cells in Recurrent Respiratory Papillomatosis (RRP) and to correlate it with severity using the Derkay laryngoscopic scale. Materials and methods: The data and biopsies from 36 patients with Juvenile (JRRP) and 56 patients with Adult (ARRP) were collected and analyzed under light microscopy. The patients were separated into groups according to the Derkay index: ≥20 for the most severe and < 20 for the less severe cases. Immunohistochemical analysis using CD3, CD4, CD8, CD15, CD20, CD68, FoxP3 and MUM-1 antibodies was performed, and the inflammatory cells were quantified. All the clinicopathological characteristics and the results of the immunohistochemical analysis were compared among the groups proposed using the Chi-Square test and correlated through the Spearman correlation test. Results: The ARRP showed significantly higher quantities of CD3+, CD8+ and MUM1+ cells (p < .05) than the JRRP samples. The presence of CD15+ cells showed positive correlation with the Derkay index (p < .05), while the MUM-1+ cells showed an inverse correlation (p = .01). Conclusion: There are differences between the inflammatory cells population in the juvenile and adult groups and it can be related to disease severity.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:07:01Z
2020-12-12T01:07:01Z
2020-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.amjoto.2019.102321
American Journal of Otolaryngology - Head and Neck Medicine and Surgery, v. 41, n. 2, 2020.
1532-818X
0196-0709
http://hdl.handle.net/11449/198226
10.1016/j.amjoto.2019.102321
2-s2.0-85075903383
url http://dx.doi.org/10.1016/j.amjoto.2019.102321
http://hdl.handle.net/11449/198226
identifier_str_mv American Journal of Otolaryngology - Head and Neck Medicine and Surgery, v. 41, n. 2, 2020.
1532-818X
0196-0709
10.1016/j.amjoto.2019.102321
2-s2.0-85075903383
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv American Journal of Otolaryngology - Head and Neck Medicine and Surgery
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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