Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis

Detalhes bibliográficos
Autor(a) principal: Costa, Victor [UNESP]
Data de Publicação: 2019
Outros Autores: El-Achkar, Vivian Narana [UNESP], de Barros, Patrícia Pimentel [UNESP], León, Jorge Esquiche, Ribeiro-Silva, Alfredo, Carlos, Román, Pignatari, Shirley Shizue Nagata, Ferreira, Silvaneide, Mello, Barbara Pereira, Sichero, Laura, Villa, Luisa Lina, Kaminagakura, Estela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/lary.28465
http://hdl.handle.net/11449/199858
Resumo: Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.
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spelling Role of epstein-barr virus in the severity of recurrent respiratory papillomatosisCD21Epstein-Barr virushuman papilloma virusseveritySquamous papillomatosisObjective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos CamposDepartment of Stomatology Collective Health and Legal Dentistry Ribeirão Preto School of DentistryDepartment of Pathology and Forensic Medicine Ribeirão Preto Medical School University of São Paulo Ribeirão PretoDepartment of Otolaryngology and Head and Neck Surgery Federal University of Sao PauloCenter for Translational Investigation in Oncology Instituto do Cancer do Estado de Sao Paulo Hospital das Clinicas da Faculdade de Medicina da Universidade de São PauloDepartment of Radiology and Oncology Faculdade de Medicina Universidade de São PauloCentro Clínico de Cabeza y CuelloDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos CamposUniversidade Estadual Paulista (Unesp)Ribeirão Preto School of DentistryUniversidade de São Paulo (USP)Centro Clínico de Cabeza y CuelloCosta, Victor [UNESP]El-Achkar, Vivian Narana [UNESP]de Barros, Patrícia Pimentel [UNESP]León, Jorge EsquicheRibeiro-Silva, AlfredoCarlos, RománPignatari, Shirley Shizue NagataFerreira, SilvaneideMello, Barbara PereiraSichero, LauraVilla, Luisa LinaKaminagakura, Estela [UNESP]2020-12-12T01:51:12Z2020-12-12T01:51:12Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/lary.28465Laryngoscope.1531-49950023-852Xhttp://hdl.handle.net/11449/19985810.1002/lary.284652-s2.0-85077019439Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLaryngoscopeinfo:eu-repo/semantics/openAccess2021-10-23T10:11:10Zoai:repositorio.unesp.br:11449/199858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:53.830546Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
title Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
spellingShingle Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
Costa, Victor [UNESP]
CD21
Epstein-Barr virus
human papilloma virus
severity
Squamous papillomatosis
title_short Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
title_full Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
title_fullStr Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
title_full_unstemmed Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
title_sort Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
author Costa, Victor [UNESP]
author_facet Costa, Victor [UNESP]
El-Achkar, Vivian Narana [UNESP]
de Barros, Patrícia Pimentel [UNESP]
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Carlos, Román
Pignatari, Shirley Shizue Nagata
Ferreira, Silvaneide
Mello, Barbara Pereira
Sichero, Laura
Villa, Luisa Lina
Kaminagakura, Estela [UNESP]
author_role author
author2 El-Achkar, Vivian Narana [UNESP]
de Barros, Patrícia Pimentel [UNESP]
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Carlos, Román
Pignatari, Shirley Shizue Nagata
Ferreira, Silvaneide
Mello, Barbara Pereira
Sichero, Laura
Villa, Luisa Lina
Kaminagakura, Estela [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Ribeirão Preto School of Dentistry
Universidade de São Paulo (USP)
Centro Clínico de Cabeza y Cuello
dc.contributor.author.fl_str_mv Costa, Victor [UNESP]
El-Achkar, Vivian Narana [UNESP]
de Barros, Patrícia Pimentel [UNESP]
León, Jorge Esquiche
Ribeiro-Silva, Alfredo
Carlos, Román
Pignatari, Shirley Shizue Nagata
Ferreira, Silvaneide
Mello, Barbara Pereira
Sichero, Laura
Villa, Luisa Lina
Kaminagakura, Estela [UNESP]
dc.subject.por.fl_str_mv CD21
Epstein-Barr virus
human papilloma virus
severity
Squamous papillomatosis
topic CD21
Epstein-Barr virus
human papilloma virus
severity
Squamous papillomatosis
description Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-12T01:51:12Z
2020-12-12T01:51:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/lary.28465
Laryngoscope.
1531-4995
0023-852X
http://hdl.handle.net/11449/199858
10.1002/lary.28465
2-s2.0-85077019439
url http://dx.doi.org/10.1002/lary.28465
http://hdl.handle.net/11449/199858
identifier_str_mv Laryngoscope.
1531-4995
0023-852X
10.1002/lary.28465
2-s2.0-85077019439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Laryngoscope
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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