Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/lary.28465 http://hdl.handle.net/11449/199858 |
Resumo: | Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019. |
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Role of epstein-barr virus in the severity of recurrent respiratory papillomatosisCD21Epstein-Barr virushuman papilloma virusseveritySquamous papillomatosisObjective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos CamposDepartment of Stomatology Collective Health and Legal Dentistry Ribeirão Preto School of DentistryDepartment of Pathology and Forensic Medicine Ribeirão Preto Medical School University of São Paulo Ribeirão PretoDepartment of Otolaryngology and Head and Neck Surgery Federal University of Sao PauloCenter for Translational Investigation in Oncology Instituto do Cancer do Estado de Sao Paulo Hospital das Clinicas da Faculdade de Medicina da Universidade de São PauloDepartment of Radiology and Oncology Faculdade de Medicina Universidade de São PauloCentro Clínico de Cabeza y CuelloDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos CamposUniversidade Estadual Paulista (Unesp)Ribeirão Preto School of DentistryUniversidade de São Paulo (USP)Centro Clínico de Cabeza y CuelloCosta, Victor [UNESP]El-Achkar, Vivian Narana [UNESP]de Barros, Patrícia Pimentel [UNESP]León, Jorge EsquicheRibeiro-Silva, AlfredoCarlos, RománPignatari, Shirley Shizue NagataFerreira, SilvaneideMello, Barbara PereiraSichero, LauraVilla, Luisa LinaKaminagakura, Estela [UNESP]2020-12-12T01:51:12Z2020-12-12T01:51:12Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/lary.28465Laryngoscope.1531-49950023-852Xhttp://hdl.handle.net/11449/19985810.1002/lary.284652-s2.0-85077019439Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLaryngoscopeinfo:eu-repo/semantics/openAccess2021-10-23T10:11:10Zoai:repositorio.unesp.br:11449/199858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:53.830546Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
title |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
spellingShingle |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis Costa, Victor [UNESP] CD21 Epstein-Barr virus human papilloma virus severity Squamous papillomatosis |
title_short |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
title_full |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
title_fullStr |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
title_full_unstemmed |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
title_sort |
Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis |
author |
Costa, Victor [UNESP] |
author_facet |
Costa, Victor [UNESP] El-Achkar, Vivian Narana [UNESP] de Barros, Patrícia Pimentel [UNESP] León, Jorge Esquiche Ribeiro-Silva, Alfredo Carlos, Román Pignatari, Shirley Shizue Nagata Ferreira, Silvaneide Mello, Barbara Pereira Sichero, Laura Villa, Luisa Lina Kaminagakura, Estela [UNESP] |
author_role |
author |
author2 |
El-Achkar, Vivian Narana [UNESP] de Barros, Patrícia Pimentel [UNESP] León, Jorge Esquiche Ribeiro-Silva, Alfredo Carlos, Román Pignatari, Shirley Shizue Nagata Ferreira, Silvaneide Mello, Barbara Pereira Sichero, Laura Villa, Luisa Lina Kaminagakura, Estela [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ribeirão Preto School of Dentistry Universidade de São Paulo (USP) Centro Clínico de Cabeza y Cuello |
dc.contributor.author.fl_str_mv |
Costa, Victor [UNESP] El-Achkar, Vivian Narana [UNESP] de Barros, Patrícia Pimentel [UNESP] León, Jorge Esquiche Ribeiro-Silva, Alfredo Carlos, Román Pignatari, Shirley Shizue Nagata Ferreira, Silvaneide Mello, Barbara Pereira Sichero, Laura Villa, Luisa Lina Kaminagakura, Estela [UNESP] |
dc.subject.por.fl_str_mv |
CD21 Epstein-Barr virus human papilloma virus severity Squamous papillomatosis |
topic |
CD21 Epstein-Barr virus human papilloma virus severity Squamous papillomatosis |
description |
Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-12T01:51:12Z 2020-12-12T01:51:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/lary.28465 Laryngoscope. 1531-4995 0023-852X http://hdl.handle.net/11449/199858 10.1002/lary.28465 2-s2.0-85077019439 |
url |
http://dx.doi.org/10.1002/lary.28465 http://hdl.handle.net/11449/199858 |
identifier_str_mv |
Laryngoscope. 1531-4995 0023-852X 10.1002/lary.28465 2-s2.0-85077019439 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Laryngoscope |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129585045307392 |