Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11046-020-00509-x http://hdl.handle.net/11449/205494 |
Resumo: | Dermatomycoses caused by Candida spp. are increasingly common, however there are few reports in the literature regarding their epidemiology, pathogenesis and antifungal susceptibility of these fungal pathogens. This study aimed to identify, characterize virulence and determine the antifungal susceptibility of Candida species isolated from patients with dermatomycoses. We studied a total of 45 yeast samples isolated from dermal scrapings from patients treated at a public hospital, Bauru, São Paulo, Brazil. After identification, the samples were analyzed for protease activity, phospholipase, biofilm formation and antifungal susceptibility. The most common species was Candida albicans (40%), followed by C. krusei (22.22%), C. parapsilosis complex (22.22%) and C. famata (6.67%). Regarding virulence mechanisms, 57.78% and 28.89% of the samples showed enzymatic activity for protease and phospholipase, respectively. A total of 13.33% of the samples showed secretion of both enzymes. All isolates were biofilm producers, and a higher production was observed in C. tropicalis isolates. Regarding the antifungal susceptibility test, 97.78% of the samples had the highest levels of the minimal inhibitory concentration (MIC) for terbinafine, and one C. krusei sample showed intermediate susceptibility to fluconazole. The correct identification of dermatomycosis-related microorganisms, as well as a better knowledge of their pathogenicity and susceptibility against the antifungals used in the clinic, is extremely important for an efficient preventive and therapeutic action. |
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Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis PatientsAntifungalsCandida sppDermatomycosesEpidemiologyPathogenesisVirulenceDermatomycoses caused by Candida spp. are increasingly common, however there are few reports in the literature regarding their epidemiology, pathogenesis and antifungal susceptibility of these fungal pathogens. This study aimed to identify, characterize virulence and determine the antifungal susceptibility of Candida species isolated from patients with dermatomycoses. We studied a total of 45 yeast samples isolated from dermal scrapings from patients treated at a public hospital, Bauru, São Paulo, Brazil. After identification, the samples were analyzed for protease activity, phospholipase, biofilm formation and antifungal susceptibility. The most common species was Candida albicans (40%), followed by C. krusei (22.22%), C. parapsilosis complex (22.22%) and C. famata (6.67%). Regarding virulence mechanisms, 57.78% and 28.89% of the samples showed enzymatic activity for protease and phospholipase, respectively. A total of 13.33% of the samples showed secretion of both enzymes. All isolates were biofilm producers, and a higher production was observed in C. tropicalis isolates. Regarding the antifungal susceptibility test, 97.78% of the samples had the highest levels of the minimal inhibitory concentration (MIC) for terbinafine, and one C. krusei sample showed intermediate susceptibility to fluconazole. The correct identification of dermatomycosis-related microorganisms, as well as a better knowledge of their pathogenicity and susceptibility against the antifungals used in the clinic, is extremely important for an efficient preventive and therapeutic action.Lauro de Souza Lima InstituteAdolfo Lutz InstituteMedical School São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)Medical School São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)Lauro de Souza Lima InstituteAdolfo Lutz InstituteUniversidade Estadual Paulista (Unesp)Mello, Victor GalvãoEscudeiro, HeloisaWeckwerth, Ana Carolina Villas BôasAndrade, Maria IzildaFusaro, Ana Elisade Moraes, Eloise Brasil [UNESP]Ruiz, Luciana da Silva [UNESP]Baptista, Ida Maria Foschiani Dias [UNESP]2021-06-25T10:16:15Z2021-06-25T10:16:15Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article71-80http://dx.doi.org/10.1007/s11046-020-00509-xMycopathologia, v. 186, n. 1, p. 71-80, 2021.1573-08320301-486Xhttp://hdl.handle.net/11449/20549410.1007/s11046-020-00509-x2-s2.0-85096380752Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMycopathologiainfo:eu-repo/semantics/openAccess2021-10-23T14:40:35Zoai:repositorio.unesp.br:11449/205494Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:40:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
title |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
spellingShingle |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients Mello, Victor Galvão Antifungals Candida spp Dermatomycoses Epidemiology Pathogenesis Virulence |
title_short |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
title_full |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
title_fullStr |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
title_full_unstemmed |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
title_sort |
Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients |
author |
Mello, Victor Galvão |
author_facet |
Mello, Victor Galvão Escudeiro, Heloisa Weckwerth, Ana Carolina Villas Bôas Andrade, Maria Izilda Fusaro, Ana Elisa de Moraes, Eloise Brasil [UNESP] Ruiz, Luciana da Silva [UNESP] Baptista, Ida Maria Foschiani Dias [UNESP] |
author_role |
author |
author2 |
Escudeiro, Heloisa Weckwerth, Ana Carolina Villas Bôas Andrade, Maria Izilda Fusaro, Ana Elisa de Moraes, Eloise Brasil [UNESP] Ruiz, Luciana da Silva [UNESP] Baptista, Ida Maria Foschiani Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Lauro de Souza Lima Institute Adolfo Lutz Institute Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mello, Victor Galvão Escudeiro, Heloisa Weckwerth, Ana Carolina Villas Bôas Andrade, Maria Izilda Fusaro, Ana Elisa de Moraes, Eloise Brasil [UNESP] Ruiz, Luciana da Silva [UNESP] Baptista, Ida Maria Foschiani Dias [UNESP] |
dc.subject.por.fl_str_mv |
Antifungals Candida spp Dermatomycoses Epidemiology Pathogenesis Virulence |
topic |
Antifungals Candida spp Dermatomycoses Epidemiology Pathogenesis Virulence |
description |
Dermatomycoses caused by Candida spp. are increasingly common, however there are few reports in the literature regarding their epidemiology, pathogenesis and antifungal susceptibility of these fungal pathogens. This study aimed to identify, characterize virulence and determine the antifungal susceptibility of Candida species isolated from patients with dermatomycoses. We studied a total of 45 yeast samples isolated from dermal scrapings from patients treated at a public hospital, Bauru, São Paulo, Brazil. After identification, the samples were analyzed for protease activity, phospholipase, biofilm formation and antifungal susceptibility. The most common species was Candida albicans (40%), followed by C. krusei (22.22%), C. parapsilosis complex (22.22%) and C. famata (6.67%). Regarding virulence mechanisms, 57.78% and 28.89% of the samples showed enzymatic activity for protease and phospholipase, respectively. A total of 13.33% of the samples showed secretion of both enzymes. All isolates were biofilm producers, and a higher production was observed in C. tropicalis isolates. Regarding the antifungal susceptibility test, 97.78% of the samples had the highest levels of the minimal inhibitory concentration (MIC) for terbinafine, and one C. krusei sample showed intermediate susceptibility to fluconazole. The correct identification of dermatomycosis-related microorganisms, as well as a better knowledge of their pathogenicity and susceptibility against the antifungals used in the clinic, is extremely important for an efficient preventive and therapeutic action. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:16:15Z 2021-06-25T10:16:15Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11046-020-00509-x Mycopathologia, v. 186, n. 1, p. 71-80, 2021. 1573-0832 0301-486X http://hdl.handle.net/11449/205494 10.1007/s11046-020-00509-x 2-s2.0-85096380752 |
url |
http://dx.doi.org/10.1007/s11046-020-00509-x http://hdl.handle.net/11449/205494 |
identifier_str_mv |
Mycopathologia, v. 186, n. 1, p. 71-80, 2021. 1573-0832 0301-486X 10.1007/s11046-020-00509-x 2-s2.0-85096380752 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mycopathologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
71-80 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046882990096384 |