Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-015-1577-2 http://hdl.handle.net/11449/161267 |
Resumo: | Objectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics. |
id |
UNSP_1cda7b859d38a5b76d42a6b9a977bbfb |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/161267 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilmElectrospinningScaffoldAntibioticDisinfectionEndodonticsNanofibersObjectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.International Development Funds (IDF) from Indiana University-Purdue University (IUPUI/OVCR)Indiana University School of Dentistry (IUSD)IUSD, Dept Biomed & Appl Sci, 1121 W Michigan St, Indianapolis, IN 46202 USAIndiana Univ Sch Med, Dept Anat & Cell Biol, Indianapolis, IN 46202 USAIndiana Univ Purdue Univ, Purdue Sch Engn & Technol, Dept Biomed Engn, Indianapolis, IN 46202 USAUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, BR-12245000 Sao Jose Dos Campos, SP, BrazilUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, BR-12245000 Sao Jose Dos Campos, SP, BrazilSpringerIUSDIndiana Univ Sch MedIndiana Univ Purdue UnivUniversidade Estadual Paulista (Unesp)Albuquerque, Maria Tereza P. [UNESP]Evans, Joshua D.Gregory, Richard L.Valera, Marcia C. [UNESP]Bottino, Marco C.2018-11-26T16:27:50Z2018-11-26T16:27:50Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-393application/pdfhttp://dx.doi.org/10.1007/s00784-015-1577-2Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016.1432-6981http://hdl.handle.net/11449/16126710.1007/s00784-015-1577-2WOS:000371426800023WOS000371426800023.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2023-10-07T06:10:05Zoai:repositorio.unesp.br:11449/161267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:17:38.819168Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
title |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
spellingShingle |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm Albuquerque, Maria Tereza P. [UNESP] Electrospinning Scaffold Antibiotic Disinfection Endodontics Nanofibers |
title_short |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
title_full |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
title_fullStr |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
title_full_unstemmed |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
title_sort |
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm |
author |
Albuquerque, Maria Tereza P. [UNESP] |
author_facet |
Albuquerque, Maria Tereza P. [UNESP] Evans, Joshua D. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
author_role |
author |
author2 |
Evans, Joshua D. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
IUSD Indiana Univ Sch Med Indiana Univ Purdue Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Albuquerque, Maria Tereza P. [UNESP] Evans, Joshua D. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
dc.subject.por.fl_str_mv |
Electrospinning Scaffold Antibiotic Disinfection Endodontics Nanofibers |
topic |
Electrospinning Scaffold Antibiotic Disinfection Endodontics Nanofibers |
description |
Objectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 2018-11-26T16:27:50Z 2018-11-26T16:27:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-015-1577-2 Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016. 1432-6981 http://hdl.handle.net/11449/161267 10.1007/s00784-015-1577-2 WOS:000371426800023 WOS000371426800023.pdf |
url |
http://dx.doi.org/10.1007/s00784-015-1577-2 http://hdl.handle.net/11449/161267 |
identifier_str_mv |
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016. 1432-6981 10.1007/s00784-015-1577-2 WOS:000371426800023 WOS000371426800023.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
387-393 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128341957410816 |