Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Maria Tereza P. [UNESP]
Data de Publicação: 2016
Outros Autores: Evans, Joshua D., Gregory, Richard L., Valera, Marcia C. [UNESP], Bottino, Marco C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-015-1577-2
http://hdl.handle.net/11449/161267
Resumo: Objectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.
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spelling Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilmElectrospinningScaffoldAntibioticDisinfectionEndodonticsNanofibersObjectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.International Development Funds (IDF) from Indiana University-Purdue University (IUPUI/OVCR)Indiana University School of Dentistry (IUSD)IUSD, Dept Biomed & Appl Sci, 1121 W Michigan St, Indianapolis, IN 46202 USAIndiana Univ Sch Med, Dept Anat & Cell Biol, Indianapolis, IN 46202 USAIndiana Univ Purdue Univ, Purdue Sch Engn & Technol, Dept Biomed Engn, Indianapolis, IN 46202 USAUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, BR-12245000 Sao Jose Dos Campos, SP, BrazilUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, BR-12245000 Sao Jose Dos Campos, SP, BrazilSpringerIUSDIndiana Univ Sch MedIndiana Univ Purdue UnivUniversidade Estadual Paulista (Unesp)Albuquerque, Maria Tereza P. [UNESP]Evans, Joshua D.Gregory, Richard L.Valera, Marcia C. [UNESP]Bottino, Marco C.2018-11-26T16:27:50Z2018-11-26T16:27:50Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-393application/pdfhttp://dx.doi.org/10.1007/s00784-015-1577-2Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016.1432-6981http://hdl.handle.net/11449/16126710.1007/s00784-015-1577-2WOS:000371426800023WOS000371426800023.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2023-10-07T06:10:05Zoai:repositorio.unesp.br:11449/161267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:17:38.819168Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
title Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
spellingShingle Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
Albuquerque, Maria Tereza P. [UNESP]
Electrospinning
Scaffold
Antibiotic
Disinfection
Endodontics
Nanofibers
title_short Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
title_full Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
title_fullStr Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
title_full_unstemmed Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
title_sort Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
author Albuquerque, Maria Tereza P. [UNESP]
author_facet Albuquerque, Maria Tereza P. [UNESP]
Evans, Joshua D.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
author_role author
author2 Evans, Joshua D.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IUSD
Indiana Univ Sch Med
Indiana Univ Purdue Univ
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Albuquerque, Maria Tereza P. [UNESP]
Evans, Joshua D.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
dc.subject.por.fl_str_mv Electrospinning
Scaffold
Antibiotic
Disinfection
Endodontics
Nanofibers
topic Electrospinning
Scaffold
Antibiotic
Disinfection
Endodontics
Nanofibers
description Objectives This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Materials and methods Dentin specimens (4 x 4 x 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP 25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level. Results Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log(10) CFU/mL) and the negative control (5.9 log10 CFU/mL). Conclusions The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. Clinical relevance Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
2018-11-26T16:27:50Z
2018-11-26T16:27:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-015-1577-2
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016.
1432-6981
http://hdl.handle.net/11449/161267
10.1007/s00784-015-1577-2
WOS:000371426800023
WOS000371426800023.pdf
url http://dx.doi.org/10.1007/s00784-015-1577-2
http://hdl.handle.net/11449/161267
identifier_str_mv Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 2, p. 387-393, 2016.
1432-6981
10.1007/s00784-015-1577-2
WOS:000371426800023
WOS000371426800023.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
0,986
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 387-393
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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