Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2015.03.005 http://hdl.handle.net/11449/160695 |
Resumo: | Introduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics. |
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Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii BiofilmAntibioticbacteriadisinfectionelectrospinningnanofiberspulpregenerationroot canalscaffoldstem cellsIntroduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.International Development Funds (IDE) Grant from Indiana University Purdue UniversityIndiana University School of DentistryIndiana Clinical and Translational Sciences InstituteNational Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences AwardIndiana Univ, Sch Dent, Dept Restorat Dent, Div Dent Biomat, Indianapolis, IN 46202 USAIndiana Univ, Sch Dent, Div Nephrol, Indianapolis, IN 46202 USAIndiana Univ, Sch Dent, Dept Oral Biol, Indianapolis, IN 46202 USAUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, Sao Paulo, BrazilFed Univ Pelotas UFPEL, Dept Operat Dent, Sch Dent, Pelotas, RS, BrazilUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, Sao Paulo, BrazilNational Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award: UL1 TR001108Elsevier B.V.Indiana UnivUniversidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Albuquerque, Maria T. P. [UNESP]Ryan, Stuart J.Muenchow, Eliseu A.Kamocka, Maria M.Gregory, Richard L.Valera, Marcia C. [UNESP]Bottino, Marco C.2018-11-26T16:16:21Z2018-11-26T16:16:21Z2015-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1337-1343application/pdfhttp://dx.doi.org/10.1016/j.joen.2015.03.005Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015.0099-2399http://hdl.handle.net/11449/16069510.1016/j.joen.2015.03.005WOS:000359183600022WOS000359183600022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Endodontics1,585info:eu-repo/semantics/openAccess2023-12-17T06:17:41Zoai:repositorio.unesp.br:11449/160695Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:32:51.972030Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
title |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
spellingShingle |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm Albuquerque, Maria T. P. [UNESP] Antibiotic bacteria disinfection electrospinning nanofibers pulp regeneration root canal scaffold stem cells |
title_short |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
title_full |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
title_fullStr |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
title_full_unstemmed |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
title_sort |
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm |
author |
Albuquerque, Maria T. P. [UNESP] |
author_facet |
Albuquerque, Maria T. P. [UNESP] Ryan, Stuart J. Muenchow, Eliseu A. Kamocka, Maria M. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
author_role |
author |
author2 |
Ryan, Stuart J. Muenchow, Eliseu A. Kamocka, Maria M. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Indiana Univ Universidade Estadual Paulista (Unesp) Universidade Federal de Pernambuco (UFPE) |
dc.contributor.author.fl_str_mv |
Albuquerque, Maria T. P. [UNESP] Ryan, Stuart J. Muenchow, Eliseu A. Kamocka, Maria M. Gregory, Richard L. Valera, Marcia C. [UNESP] Bottino, Marco C. |
dc.subject.por.fl_str_mv |
Antibiotic bacteria disinfection electrospinning nanofibers pulp regeneration root canal scaffold stem cells |
topic |
Antibiotic bacteria disinfection electrospinning nanofibers pulp regeneration root canal scaffold stem cells |
description |
Introduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 2018-11-26T16:16:21Z 2018-11-26T16:16:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2015.03.005 Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015. 0099-2399 http://hdl.handle.net/11449/160695 10.1016/j.joen.2015.03.005 WOS:000359183600022 WOS000359183600022.pdf |
url |
http://dx.doi.org/10.1016/j.joen.2015.03.005 http://hdl.handle.net/11449/160695 |
identifier_str_mv |
Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015. 0099-2399 10.1016/j.joen.2015.03.005 WOS:000359183600022 WOS000359183600022.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Endodontics 1,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1337-1343 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129218421194752 |