Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Maria T. P. [UNESP]
Data de Publicação: 2015
Outros Autores: Ryan, Stuart J., Muenchow, Eliseu A., Kamocka, Maria M., Gregory, Richard L., Valera, Marcia C. [UNESP], Bottino, Marco C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.joen.2015.03.005
http://hdl.handle.net/11449/160695
Resumo: Introduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.
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spelling Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii BiofilmAntibioticbacteriadisinfectionelectrospinningnanofiberspulpregenerationroot canalscaffoldstem cellsIntroduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.International Development Funds (IDE) Grant from Indiana University Purdue UniversityIndiana University School of DentistryIndiana Clinical and Translational Sciences InstituteNational Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences AwardIndiana Univ, Sch Dent, Dept Restorat Dent, Div Dent Biomat, Indianapolis, IN 46202 USAIndiana Univ, Sch Dent, Div Nephrol, Indianapolis, IN 46202 USAIndiana Univ, Sch Dent, Dept Oral Biol, Indianapolis, IN 46202 USAUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, Sao Paulo, BrazilFed Univ Pelotas UFPEL, Dept Operat Dent, Sch Dent, Pelotas, RS, BrazilUniv Estadual Paulista, Sao Jose dos Campos Dent Sch, Grad Program Restorat Dent Endodont, Sao Paulo, BrazilNational Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award: UL1 TR001108Elsevier B.V.Indiana UnivUniversidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Albuquerque, Maria T. P. [UNESP]Ryan, Stuart J.Muenchow, Eliseu A.Kamocka, Maria M.Gregory, Richard L.Valera, Marcia C. [UNESP]Bottino, Marco C.2018-11-26T16:16:21Z2018-11-26T16:16:21Z2015-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1337-1343application/pdfhttp://dx.doi.org/10.1016/j.joen.2015.03.005Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015.0099-2399http://hdl.handle.net/11449/16069510.1016/j.joen.2015.03.005WOS:000359183600022WOS000359183600022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Endodontics1,585info:eu-repo/semantics/openAccess2023-12-17T06:17:41Zoai:repositorio.unesp.br:11449/160695Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:32:51.972030Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
title Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
spellingShingle Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
Albuquerque, Maria T. P. [UNESP]
Antibiotic
bacteria
disinfection
electrospinning
nanofibers
pulp
regeneration
root canal
scaffold
stem cells
title_short Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
title_full Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
title_fullStr Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
title_full_unstemmed Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
title_sort Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm
author Albuquerque, Maria T. P. [UNESP]
author_facet Albuquerque, Maria T. P. [UNESP]
Ryan, Stuart J.
Muenchow, Eliseu A.
Kamocka, Maria M.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
author_role author
author2 Ryan, Stuart J.
Muenchow, Eliseu A.
Kamocka, Maria M.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Indiana Univ
Universidade Estadual Paulista (Unesp)
Universidade Federal de Pernambuco (UFPE)
dc.contributor.author.fl_str_mv Albuquerque, Maria T. P. [UNESP]
Ryan, Stuart J.
Muenchow, Eliseu A.
Kamocka, Maria M.
Gregory, Richard L.
Valera, Marcia C. [UNESP]
Bottino, Marco C.
dc.subject.por.fl_str_mv Antibiotic
bacteria
disinfection
electrospinning
nanofibers
pulp
regeneration
root canal
scaffold
stem cells
topic Antibiotic
bacteria
disinfection
electrospinning
nanofibers
pulp
regeneration
root canal
scaffold
stem cells
description Introduction: Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP) mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods: Metronidazole, ciprofloxacin, and minocydine were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 x 4 x 1 mm(3), n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens wete exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results: Scaffolds displayed a submicron mean fiber diameter (PDS = 689 +/- 12 nm and TAP-mimic = 718 +/- 125 nm). Overall, TAP-mimic scaffolds showed significantly (P <= 040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions: Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
2018-11-26T16:16:21Z
2018-11-26T16:16:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.joen.2015.03.005
Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015.
0099-2399
http://hdl.handle.net/11449/160695
10.1016/j.joen.2015.03.005
WOS:000359183600022
WOS000359183600022.pdf
url http://dx.doi.org/10.1016/j.joen.2015.03.005
http://hdl.handle.net/11449/160695
identifier_str_mv Journal Of Endodontics. New York: Elsevier Science Inc, v. 41, n. 8, p. 1337-1343, 2015.
0099-2399
10.1016/j.joen.2015.03.005
WOS:000359183600022
WOS000359183600022.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Endodontics
1,585
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1337-1343
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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