Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy

Detalhes bibliográficos
Autor(a) principal: Vesentini, G. [UNESP]
Data de Publicação: 2018
Outros Autores: Marini, G. [UNESP], Piculo, F. [UNESP], Damasceno, D. C. [UNESP], Matheus, S. M. M. [UNESP], Felisbino, S. L. [UNESP], Calderon, I. M. P. [UNESP], Hijaz, A., Barbosa, A. M. P. [UNESP], Rudge, M. V. C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1414-431X20177035
http://hdl.handle.net/11449/163922
Resumo: The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy.
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spelling Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancyDiabetesPregnancyCollagenSkeletal muscleRatsThe urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilUniv Sagrado Coracao, Dept Ciencias Saude, Sagrado, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Hosp Case Med Ctr, Urol Inst, Dept Urol, Cleveland, OH USAUniv Estadual Paulista, Dept Fisioterapia & Terapia Ocupac, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Fisioterapia & Terapia Ocupac, Botucatu, SP, BrazilFAPESP: FAPESP 2012/25053-7FAPESP: 2014/14144-7Assoc Bras Divulg CientificaUniversidade Estadual Paulista (Unesp)Univ Sagrado CoracaoUniv Hosp Case Med CtrVesentini, G. [UNESP]Marini, G. [UNESP]Piculo, F. [UNESP]Damasceno, D. C. [UNESP]Matheus, S. M. M. [UNESP]Felisbino, S. L. [UNESP]Calderon, I. M. P. [UNESP]Hijaz, A.Barbosa, A. M. P. [UNESP]Rudge, M. V. C. [UNESP]2018-11-26T17:48:26Z2018-11-26T17:48:26Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1590/1414-431X20177035Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018.0100-879Xhttp://hdl.handle.net/11449/16392210.1590/1414-431X20177035S0100-879X2018000400611WOS:000426394200001S0100-879X2018000400611.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Medical And Biological Researchinfo:eu-repo/semantics/openAccess2024-08-16T14:06:42Zoai:repositorio.unesp.br:11449/163922Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
title Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
spellingShingle Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
Vesentini, G. [UNESP]
Diabetes
Pregnancy
Collagen
Skeletal muscle
Rats
title_short Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
title_full Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
title_fullStr Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
title_full_unstemmed Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
title_sort Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
author Vesentini, G. [UNESP]
author_facet Vesentini, G. [UNESP]
Marini, G. [UNESP]
Piculo, F. [UNESP]
Damasceno, D. C. [UNESP]
Matheus, S. M. M. [UNESP]
Felisbino, S. L. [UNESP]
Calderon, I. M. P. [UNESP]
Hijaz, A.
Barbosa, A. M. P. [UNESP]
Rudge, M. V. C. [UNESP]
author_role author
author2 Marini, G. [UNESP]
Piculo, F. [UNESP]
Damasceno, D. C. [UNESP]
Matheus, S. M. M. [UNESP]
Felisbino, S. L. [UNESP]
Calderon, I. M. P. [UNESP]
Hijaz, A.
Barbosa, A. M. P. [UNESP]
Rudge, M. V. C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Sagrado Coracao
Univ Hosp Case Med Ctr
dc.contributor.author.fl_str_mv Vesentini, G. [UNESP]
Marini, G. [UNESP]
Piculo, F. [UNESP]
Damasceno, D. C. [UNESP]
Matheus, S. M. M. [UNESP]
Felisbino, S. L. [UNESP]
Calderon, I. M. P. [UNESP]
Hijaz, A.
Barbosa, A. M. P. [UNESP]
Rudge, M. V. C. [UNESP]
dc.subject.por.fl_str_mv Diabetes
Pregnancy
Collagen
Skeletal muscle
Rats
topic Diabetes
Pregnancy
Collagen
Skeletal muscle
Rats
description The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T17:48:26Z
2018-11-26T17:48:26Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1414-431X20177035
Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018.
0100-879X
http://hdl.handle.net/11449/163922
10.1590/1414-431X20177035
S0100-879X2018000400611
WOS:000426394200001
S0100-879X2018000400611.pdf
url http://dx.doi.org/10.1590/1414-431X20177035
http://hdl.handle.net/11449/163922
identifier_str_mv Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018.
0100-879X
10.1590/1414-431X20177035
S0100-879X2018000400611
WOS:000426394200001
S0100-879X2018000400611.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Medical And Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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