Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1414-431X20177035 http://hdl.handle.net/11449/163922 |
Resumo: | The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy. |
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Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancyDiabetesPregnancyCollagenSkeletal muscleRatsThe urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilUniv Sagrado Coracao, Dept Ciencias Saude, Sagrado, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Hosp Case Med Ctr, Urol Inst, Dept Urol, Cleveland, OH USAUniv Estadual Paulista, Dept Fisioterapia & Terapia Ocupac, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Fisioterapia & Terapia Ocupac, Botucatu, SP, BrazilFAPESP: FAPESP 2012/25053-7FAPESP: 2014/14144-7Assoc Bras Divulg CientificaUniversidade Estadual Paulista (Unesp)Univ Sagrado CoracaoUniv Hosp Case Med CtrVesentini, G. [UNESP]Marini, G. [UNESP]Piculo, F. [UNESP]Damasceno, D. C. [UNESP]Matheus, S. M. M. [UNESP]Felisbino, S. L. [UNESP]Calderon, I. M. P. [UNESP]Hijaz, A.Barbosa, A. M. P. [UNESP]Rudge, M. V. C. [UNESP]2018-11-26T17:48:26Z2018-11-26T17:48:26Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1590/1414-431X20177035Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018.0100-879Xhttp://hdl.handle.net/11449/16392210.1590/1414-431X20177035S0100-879X2018000400611WOS:000426394200001S0100-879X2018000400611.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Medical And Biological Researchinfo:eu-repo/semantics/openAccess2024-08-16T14:06:42Zoai:repositorio.unesp.br:11449/163922Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
title |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
spellingShingle |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy Vesentini, G. [UNESP] Diabetes Pregnancy Collagen Skeletal muscle Rats |
title_short |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
title_full |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
title_fullStr |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
title_full_unstemmed |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
title_sort |
Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy |
author |
Vesentini, G. [UNESP] |
author_facet |
Vesentini, G. [UNESP] Marini, G. [UNESP] Piculo, F. [UNESP] Damasceno, D. C. [UNESP] Matheus, S. M. M. [UNESP] Felisbino, S. L. [UNESP] Calderon, I. M. P. [UNESP] Hijaz, A. Barbosa, A. M. P. [UNESP] Rudge, M. V. C. [UNESP] |
author_role |
author |
author2 |
Marini, G. [UNESP] Piculo, F. [UNESP] Damasceno, D. C. [UNESP] Matheus, S. M. M. [UNESP] Felisbino, S. L. [UNESP] Calderon, I. M. P. [UNESP] Hijaz, A. Barbosa, A. M. P. [UNESP] Rudge, M. V. C. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Sagrado Coracao Univ Hosp Case Med Ctr |
dc.contributor.author.fl_str_mv |
Vesentini, G. [UNESP] Marini, G. [UNESP] Piculo, F. [UNESP] Damasceno, D. C. [UNESP] Matheus, S. M. M. [UNESP] Felisbino, S. L. [UNESP] Calderon, I. M. P. [UNESP] Hijaz, A. Barbosa, A. M. P. [UNESP] Rudge, M. V. C. [UNESP] |
dc.subject.por.fl_str_mv |
Diabetes Pregnancy Collagen Skeletal muscle Rats |
topic |
Diabetes Pregnancy Collagen Skeletal muscle Rats |
description |
The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for fast and slow muscle fibers and transmission electron microscopy. We investigated two levels of STZ-induced diabetes: long-term mild diabetes (blood glucose level: 120-200 mg/dL) and short-term severe diabetes (blood glucose level 4300 mg/dL). Long-term mild diabetic pregnant and short-term severe diabetic pregnant rats had decreased fast fibers and increased slow fibers, disrupted areas of sarcomere, intermyofibrillar mitochondria and myelin figures in the RA muscle. Both groups enabled us to analyze the specific influence of pregnancy, separately from diabetes. The current study demonstrated that diabetes and pregnancy induced intramuscular transformation and reorganization of RA muscle with a switch of fiber type adjusting their architecture according to intensity and duration of hyperglycemic insult within pregnancy. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:48:26Z 2018-11-26T17:48:26Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X20177035 Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018. 0100-879X http://hdl.handle.net/11449/163922 10.1590/1414-431X20177035 S0100-879X2018000400611 WOS:000426394200001 S0100-879X2018000400611.pdf |
url |
http://dx.doi.org/10.1590/1414-431X20177035 http://hdl.handle.net/11449/163922 |
identifier_str_mv |
Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 51, n. 4, 10 p., 2018. 0100-879X 10.1590/1414-431X20177035 S0100-879X2018000400611 WOS:000426394200001 S0100-879X2018000400611.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Medical And Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128118399959040 |