Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s2175-97902018000317554 http://hdl.handle.net/11449/185171 |
Resumo: | Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil. ERIC-PCR rendered banding patterns with great diversity (1 to 12 bands) of molecular sizes, ranging from 100 to 1600 bp. ERIC-PCR showed to be fast, simple and affordable to differentiate isolates. ERIC-PCR would be an important tool in the epidemiology of TB as screening in case of outbreak, which demands rapid intervention. However if any doubt persist, as it may occur with the application of only one genotypic method, other genotyping methods should be applied and carefully interpreted, always with additional epidemiological information. |
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Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCRTuberculosisMolecular characterizationERIC-PCREpidemiologyTuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil. ERIC-PCR rendered banding patterns with great diversity (1 to 12 bands) of molecular sizes, ranging from 100 to 1600 bp. ERIC-PCR showed to be fast, simple and affordable to differentiate isolates. ERIC-PCR would be an important tool in the epidemiology of TB as screening in case of outbreak, which demands rapid intervention. However if any doubt persist, as it may occur with the application of only one genotypic method, other genotyping methods should be applied and carefully interpreted, always with additional epidemiological information.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Maringa, Dept Clin Anal & Biomed, Lab Med Bacteriol, Maringa, Parana, BrazilUniv Estadual Maringa, Postgrad Biosci & Fisiopathol, Maringa, Parana, BrazilUniv Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, Araraquara, SP, BrazilUniv Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, BrazilUniv Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, Araraquara, SP, BrazilUniv Sao Paulo, Conjunto QuimicasUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Caleffi-Ferracioli, Katiany RizzieriBaldin, Vanessa PietrowskiNoguti, Erika NodaDias Siqueira, Vera LuciaLima Scodro, Regiane BertinPavan, Fernando Rogerio [UNESP]Hirata, Mario HiroyukiCardoso, Rosilene Fressatti2019-10-04T12:33:11Z2019-10-04T12:33:11Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1590/s2175-97902018000317554Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 54, n. 3, 7 p., 2018.1984-8250http://hdl.handle.net/11449/18517110.1590/s2175-97902018000317554S1984-82502018000300616WOS:000452196300001S1984-82502018000300616.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccess2024-06-24T13:07:14Zoai:repositorio.unesp.br:11449/185171Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T13:07:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
title |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
spellingShingle |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR Caleffi-Ferracioli, Katiany Rizzieri Tuberculosis Molecular characterization ERIC-PCR Epidemiology |
title_short |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
title_full |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
title_fullStr |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
title_full_unstemmed |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
title_sort |
Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR |
author |
Caleffi-Ferracioli, Katiany Rizzieri |
author_facet |
Caleffi-Ferracioli, Katiany Rizzieri Baldin, Vanessa Pietrowski Noguti, Erika Noda Dias Siqueira, Vera Lucia Lima Scodro, Regiane Bertin Pavan, Fernando Rogerio [UNESP] Hirata, Mario Hiroyuki Cardoso, Rosilene Fressatti |
author_role |
author |
author2 |
Baldin, Vanessa Pietrowski Noguti, Erika Noda Dias Siqueira, Vera Lucia Lima Scodro, Regiane Bertin Pavan, Fernando Rogerio [UNESP] Hirata, Mario Hiroyuki Cardoso, Rosilene Fressatti |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Caleffi-Ferracioli, Katiany Rizzieri Baldin, Vanessa Pietrowski Noguti, Erika Noda Dias Siqueira, Vera Lucia Lima Scodro, Regiane Bertin Pavan, Fernando Rogerio [UNESP] Hirata, Mario Hiroyuki Cardoso, Rosilene Fressatti |
dc.subject.por.fl_str_mv |
Tuberculosis Molecular characterization ERIC-PCR Epidemiology |
topic |
Tuberculosis Molecular characterization ERIC-PCR Epidemiology |
description |
Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil. ERIC-PCR rendered banding patterns with great diversity (1 to 12 bands) of molecular sizes, ranging from 100 to 1600 bp. ERIC-PCR showed to be fast, simple and affordable to differentiate isolates. ERIC-PCR would be an important tool in the epidemiology of TB as screening in case of outbreak, which demands rapid intervention. However if any doubt persist, as it may occur with the application of only one genotypic method, other genotyping methods should be applied and carefully interpreted, always with additional epidemiological information. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2019-10-04T12:33:11Z 2019-10-04T12:33:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s2175-97902018000317554 Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 54, n. 3, 7 p., 2018. 1984-8250 http://hdl.handle.net/11449/185171 10.1590/s2175-97902018000317554 S1984-82502018000300616 WOS:000452196300001 S1984-82502018000300616.pdf |
url |
http://dx.doi.org/10.1590/s2175-97902018000317554 http://hdl.handle.net/11449/185171 |
identifier_str_mv |
Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 54, n. 3, 7 p., 2018. 1984-8250 10.1590/s2175-97902018000317554 S1984-82502018000300616 WOS:000452196300001 S1984-82502018000300616.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Pharmaceutical Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo, Conjunto Quimicas |
publisher.none.fl_str_mv |
Univ Sao Paulo, Conjunto Quimicas |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045263751774208 |