Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Detalhes bibliográficos
Autor(a) principal: Spositto,F.L.E.
Data de Publicação: 2014
Outros Autores: Campanerut,P.A.Z., Ghiraldi,L.D., Leite,C.Q.F., Hirata,M.H., Hirata,R.D.C., Siqueira,V.L.D., Cardoso,R. Fressatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012
Resumo: We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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spelling Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complexbovine tuberculosistuberculosisPCR-multiplexdiagnosisWe evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.Sociedade Brasileira de Microbiologia2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012Brazilian Journal of Microbiology v.45 n.3 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000300012info:eu-repo/semantics/openAccessSpositto,F.L.E.Campanerut,P.A.Z.Ghiraldi,L.D.Leite,C.Q.F.Hirata,M.H.Hirata,R.D.C.Siqueira,V.L.D.Cardoso,R. Fressattieng2014-11-04T00:00:00Zoai:scielo:S1517-83822014000300012Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-11-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
spellingShingle Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
Spositto,F.L.E.
bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
title_short Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_fullStr Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full_unstemmed Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_sort Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
author Spositto,F.L.E.
author_facet Spositto,F.L.E.
Campanerut,P.A.Z.
Ghiraldi,L.D.
Leite,C.Q.F.
Hirata,M.H.
Hirata,R.D.C.
Siqueira,V.L.D.
Cardoso,R. Fressatti
author_role author
author2 Campanerut,P.A.Z.
Ghiraldi,L.D.
Leite,C.Q.F.
Hirata,M.H.
Hirata,R.D.C.
Siqueira,V.L.D.
Cardoso,R. Fressatti
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Spositto,F.L.E.
Campanerut,P.A.Z.
Ghiraldi,L.D.
Leite,C.Q.F.
Hirata,M.H.
Hirata,R.D.C.
Siqueira,V.L.D.
Cardoso,R. Fressatti
dc.subject.por.fl_str_mv bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
topic bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
description We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000300012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.3 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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