Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scitotenv.2022.158086 http://hdl.handle.net/11449/242160 |
Resumo: | The concern about pesticide exposure to neotropical bees has been increasing in the last few years, and knowledge gaps have been identified. Although stingless bees, (e.g.: Melipona scutellaris), are more diverse than honeybees and they stand out in the pollination of several valuable economical crops, toxicity assessments with stingless bees are still scarce. Nowadays new approaches in ecotoxicological studies, such as omic analysis, were pointed out as a strategy to reveal mechanisms of how bees deal with these stressors. To date, no molecular techniques have been applied for the evaluation of target and/or non-target organs in stingless bees, such as the Malpighian tubules (Mt). Therefore, in the present study, we evaluated the differentially expressed genes (DEGs) in the Mt of M. scutellaris after one and eight days of exposure to LC50/100 (0.000543 ng a.i./μL) of thiamethoxam (TMX). Through functional annotation analysis of four transcriptome libraries, the time course line approach revealed 237 DEGs (nine clusters) associated with carbon/energy metabolism and cellular processes (lysosomes, autophagy, and glycan degradation). The expression profiles of Mt were altered by TMX in processes, such as detoxification, excretion, tissue regeneration, oxidative stress, apoptosis, and DNA repair. Transcriptome analysis showed that cell metabolism in Mt was mainly affected after 8 days of exposure. Nine genes were selected from different clusters and validated by RT-qPCR. According to our findings, TMX promotes several types of damage in Mt cells at the molecular level. Therefore, interference of different cellular processes directly affects the health of M. scutellaris by compromising the function of Mt. |
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Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damagesDe novo assemblyDifferentially gene expressedExcretion organNeonicotinoidSublethal effectsThe concern about pesticide exposure to neotropical bees has been increasing in the last few years, and knowledge gaps have been identified. Although stingless bees, (e.g.: Melipona scutellaris), are more diverse than honeybees and they stand out in the pollination of several valuable economical crops, toxicity assessments with stingless bees are still scarce. Nowadays new approaches in ecotoxicological studies, such as omic analysis, were pointed out as a strategy to reveal mechanisms of how bees deal with these stressors. To date, no molecular techniques have been applied for the evaluation of target and/or non-target organs in stingless bees, such as the Malpighian tubules (Mt). Therefore, in the present study, we evaluated the differentially expressed genes (DEGs) in the Mt of M. scutellaris after one and eight days of exposure to LC50/100 (0.000543 ng a.i./μL) of thiamethoxam (TMX). Through functional annotation analysis of four transcriptome libraries, the time course line approach revealed 237 DEGs (nine clusters) associated with carbon/energy metabolism and cellular processes (lysosomes, autophagy, and glycan degradation). The expression profiles of Mt were altered by TMX in processes, such as detoxification, excretion, tissue regeneration, oxidative stress, apoptosis, and DNA repair. Transcriptome analysis showed that cell metabolism in Mt was mainly affected after 8 days of exposure. Nine genes were selected from different clusters and validated by RT-qPCR. According to our findings, TMX promotes several types of damage in Mt cells at the molecular level. Therefore, interference of different cellular processes directly affects the health of M. scutellaris by compromising the function of Mt.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SPCenter of Agrarian Sciences Federal University of São Carlos (UFSCar), Anhanguera Road Km 174Department of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SPCNPq: 170714/2017-9FAPESP: 2017/21097-3FAPESP: 2020/03527-3FAPESP: 2021/01359-9CNPq: 400540/2018-5CAPES: 88887.310764/2018-00CAPES: 88887.572722/2020-00Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Miotelo, Lucas [UNESP]Ferro, Milene [UNESP]Maloni, Geovana [UNESP]Otero, Igor Vinicius Ramos [UNESP]Nocelli, Roberta Cornélio FerreiraBacci, Mauricio [UNESP]Malaspina, Osmar [UNESP]2023-03-02T10:43:48Z2023-03-02T10:43:48Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.scitotenv.2022.158086Science of the Total Environment, v. 850.1879-10260048-9697http://hdl.handle.net/11449/24216010.1016/j.scitotenv.2022.1580862-s2.0-85136135934Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScience of the Total Environmentinfo:eu-repo/semantics/openAccess2023-03-02T10:43:48Zoai:repositorio.unesp.br:11449/242160Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:59:44.490391Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
title |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
spellingShingle |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages Miotelo, Lucas [UNESP] De novo assembly Differentially gene expressed Excretion organ Neonicotinoid Sublethal effects |
title_short |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
title_full |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
title_fullStr |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
title_full_unstemmed |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
title_sort |
Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages |
author |
Miotelo, Lucas [UNESP] |
author_facet |
Miotelo, Lucas [UNESP] Ferro, Milene [UNESP] Maloni, Geovana [UNESP] Otero, Igor Vinicius Ramos [UNESP] Nocelli, Roberta Cornélio Ferreira Bacci, Mauricio [UNESP] Malaspina, Osmar [UNESP] |
author_role |
author |
author2 |
Ferro, Milene [UNESP] Maloni, Geovana [UNESP] Otero, Igor Vinicius Ramos [UNESP] Nocelli, Roberta Cornélio Ferreira Bacci, Mauricio [UNESP] Malaspina, Osmar [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Miotelo, Lucas [UNESP] Ferro, Milene [UNESP] Maloni, Geovana [UNESP] Otero, Igor Vinicius Ramos [UNESP] Nocelli, Roberta Cornélio Ferreira Bacci, Mauricio [UNESP] Malaspina, Osmar [UNESP] |
dc.subject.por.fl_str_mv |
De novo assembly Differentially gene expressed Excretion organ Neonicotinoid Sublethal effects |
topic |
De novo assembly Differentially gene expressed Excretion organ Neonicotinoid Sublethal effects |
description |
The concern about pesticide exposure to neotropical bees has been increasing in the last few years, and knowledge gaps have been identified. Although stingless bees, (e.g.: Melipona scutellaris), are more diverse than honeybees and they stand out in the pollination of several valuable economical crops, toxicity assessments with stingless bees are still scarce. Nowadays new approaches in ecotoxicological studies, such as omic analysis, were pointed out as a strategy to reveal mechanisms of how bees deal with these stressors. To date, no molecular techniques have been applied for the evaluation of target and/or non-target organs in stingless bees, such as the Malpighian tubules (Mt). Therefore, in the present study, we evaluated the differentially expressed genes (DEGs) in the Mt of M. scutellaris after one and eight days of exposure to LC50/100 (0.000543 ng a.i./μL) of thiamethoxam (TMX). Through functional annotation analysis of four transcriptome libraries, the time course line approach revealed 237 DEGs (nine clusters) associated with carbon/energy metabolism and cellular processes (lysosomes, autophagy, and glycan degradation). The expression profiles of Mt were altered by TMX in processes, such as detoxification, excretion, tissue regeneration, oxidative stress, apoptosis, and DNA repair. Transcriptome analysis showed that cell metabolism in Mt was mainly affected after 8 days of exposure. Nine genes were selected from different clusters and validated by RT-qPCR. According to our findings, TMX promotes several types of damage in Mt cells at the molecular level. Therefore, interference of different cellular processes directly affects the health of M. scutellaris by compromising the function of Mt. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-02T10:43:48Z 2023-03-02T10:43:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.scitotenv.2022.158086 Science of the Total Environment, v. 850. 1879-1026 0048-9697 http://hdl.handle.net/11449/242160 10.1016/j.scitotenv.2022.158086 2-s2.0-85136135934 |
url |
http://dx.doi.org/10.1016/j.scitotenv.2022.158086 http://hdl.handle.net/11449/242160 |
identifier_str_mv |
Science of the Total Environment, v. 850. 1879-1026 0048-9697 10.1016/j.scitotenv.2022.158086 2-s2.0-85136135934 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Science of the Total Environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129479716896768 |