A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12866-022-02454-9 http://hdl.handle.net/11449/223437 |
Resumo: | Background: Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). Results: This study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography—tandem mass spectrometry (HPLC–MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL−1 for total CKs, and 0.46 to 82.16 pmol mL−1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected. Conclusions: To better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations. |
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A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones2-methylthio-ZeatinHPLC–MS/MSMethanolPlant growth promoting bacteriatrans-ZeatinBackground: Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). Results: This study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography—tandem mass spectrometry (HPLC–MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL−1 for total CKs, and 0.46 to 82.16 pmol mL−1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected. Conclusions: To better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations.Agricultural Adaptation CouncilGenome CanadaOntario Genomics InstituteCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of CanadaGrain Farmers of OntarioEnvironmental and Life Sciences Graduate Program Trent University, 1600 West Bank DriveDepartment of Biology Trent University, 1600 West Bank DriveDepartment of Technology Sao Paulo State UniversityDepartment of Technology Sao Paulo State UniversityAgricultural Adaptation Council: ON-RP3Genome Canada: ON-RP3Ontario Genomics Institute: ON-RP3Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada: RGPIN-2018-05436Natural Sciences and Engineering Research Council of Canada: RGPIN-2018-05436Grain Farmers of Ontario: S2013AG04Trent UniversityUniversidade Estadual Paulista (UNESP)Palberg, DanielKisiała, AnnaJorge, Gabriel Lemes [UNESP]Emery, R. J. Neil2022-04-28T19:50:42Z2022-04-28T19:50:42Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12866-022-02454-9BMC Microbiology, v. 22, n. 1, 2022.1471-2180http://hdl.handle.net/11449/22343710.1186/s12866-022-02454-92-s2.0-85124323314Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T19:50:42Zoai:repositorio.unesp.br:11449/223437Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:21:56.699586Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
title |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
spellingShingle |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones Palberg, Daniel 2-methylthio-Zeatin HPLC–MS/MS Methanol Plant growth promoting bacteria trans-Zeatin |
title_short |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
title_full |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
title_fullStr |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
title_full_unstemmed |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
title_sort |
A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones |
author |
Palberg, Daniel |
author_facet |
Palberg, Daniel Kisiała, Anna Jorge, Gabriel Lemes [UNESP] Emery, R. J. Neil |
author_role |
author |
author2 |
Kisiała, Anna Jorge, Gabriel Lemes [UNESP] Emery, R. J. Neil |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Trent University Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Palberg, Daniel Kisiała, Anna Jorge, Gabriel Lemes [UNESP] Emery, R. J. Neil |
dc.subject.por.fl_str_mv |
2-methylthio-Zeatin HPLC–MS/MS Methanol Plant growth promoting bacteria trans-Zeatin |
topic |
2-methylthio-Zeatin HPLC–MS/MS Methanol Plant growth promoting bacteria trans-Zeatin |
description |
Background: Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). Results: This study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography—tandem mass spectrometry (HPLC–MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL−1 for total CKs, and 0.46 to 82.16 pmol mL−1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected. Conclusions: To better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:42Z 2022-04-28T19:50:42Z 2022-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12866-022-02454-9 BMC Microbiology, v. 22, n. 1, 2022. 1471-2180 http://hdl.handle.net/11449/223437 10.1186/s12866-022-02454-9 2-s2.0-85124323314 |
url |
http://dx.doi.org/10.1186/s12866-022-02454-9 http://hdl.handle.net/11449/223437 |
identifier_str_mv |
BMC Microbiology, v. 22, n. 1, 2022. 1471-2180 10.1186/s12866-022-02454-9 2-s2.0-85124323314 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129510996967424 |