Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-89132008000200001 http://hdl.handle.net/11449/225218 |
Resumo: | This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25° C and in a bioreactor with four complete rotations daily at 25° C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor. |
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Repositório Institucional da UNESP |
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Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activityAgricultural residuesEnzymesShiitakeThis work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25° C and in a bioreactor with four complete rotations daily at 25° C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor.Department of Chemistry and Biochemistry IB São Paulo State University (UNESP), 18618-000 Botucatu, SPAgrobiology and Agrochemistry Department Tuscia University, via S. Camillo di Lellis, s.n., 01100 ViterboDepartment of Chemistry and Biochemistry IB São Paulo State University (UNESP), 18618-000 Botucatu, SPUniversidade Estadual Paulista (UNESP)Tuscia UniversityRegina, Magali [UNESP]Broetto, Fernando [UNESP]Giovannozzi-Sermanni, GiovanniMarabotini, RositaPeranni, Claudio2022-04-28T20:42:40Z2022-04-28T20:42:40Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article223-233http://dx.doi.org/10.1590/S1516-89132008000200001Brazilian Archives of Biology and Technology, v. 51, n. 2, p. 223-233, 2008.1516-8913http://hdl.handle.net/11449/22521810.1590/S1516-891320080002000012-s2.0-47049118218Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technologyinfo:eu-repo/semantics/openAccess2022-04-28T20:42:40Zoai:repositorio.unesp.br:11449/225218Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:48:34.612189Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
title |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
spellingShingle |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity Regina, Magali [UNESP] Agricultural residues Enzymes Shiitake |
title_short |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
title_full |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
title_fullStr |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
title_full_unstemmed |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
title_sort |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulolitic activity |
author |
Regina, Magali [UNESP] |
author_facet |
Regina, Magali [UNESP] Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
author_role |
author |
author2 |
Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Tuscia University |
dc.contributor.author.fl_str_mv |
Regina, Magali [UNESP] Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
dc.subject.por.fl_str_mv |
Agricultural residues Enzymes Shiitake |
topic |
Agricultural residues Enzymes Shiitake |
description |
This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25° C and in a bioreactor with four complete rotations daily at 25° C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-28T20:42:40Z 2022-04-28T20:42:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-89132008000200001 Brazilian Archives of Biology and Technology, v. 51, n. 2, p. 223-233, 2008. 1516-8913 http://hdl.handle.net/11449/225218 10.1590/S1516-89132008000200001 2-s2.0-47049118218 |
url |
http://dx.doi.org/10.1590/S1516-89132008000200001 http://hdl.handle.net/11449/225218 |
identifier_str_mv |
Brazilian Archives of Biology and Technology, v. 51, n. 2, p. 223-233, 2008. 1516-8913 10.1590/S1516-89132008000200001 2-s2.0-47049118218 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Archives of Biology and Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
223-233 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129120017580032 |