Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/gmr18977 http://hdl.handle.net/11449/237653 |
Resumo: | The Brazilian Testudinidae family is widespread across South America. It includes Chelonoidis denticulatus, the largest tortoise in South America and Chelonoidis carbonarius, found mostly in the north and northwestern part of the continent. Using hemoglobin to identify species is cheaper than other methods such as DNA sequencing and can offer useful information, since the hemoglobin molecule is a well-preserved protein chain during the evolution of species. Thus, in order to establish a hemoglobin profile for the Brazilian Testudinidae C. denticulatus, C. carbonarius and morphotype 1, hemoglobin electrophoresis was performed at acid pH in phosphate agar and at alkaline pH in cellulose acetate, in order to visualize the specific fractions of each species. High performance liquid chromatography was used for the quantification of fractions. For an in-depth analysis and better detailing of the hemoglobin profile of the species, polypeptide chain electrophoresis was performed at acid and alkaline pH. We observed differences in the hemoglobin profiles of C. denticulatus in relation to C. carbonarius and morphotype 1, which suggests that this methodology, not common in taxonomic studies, can help determine relationships between species, since hemoglobins are proteins with well-preserved genes. We found differences in hemoglobin mobility between C. denticulatus, C. carbonarius and morphotype 1 in electrophoresis at alkaline pH, however, the behavior of globin chains was similar between the three groups. High performance liquid chromatography showed different retention times in the globin fractions of C. denticulatus and C. carbonarius, but not between C. carbonarius and morphotype 1, indicating that, possibly, the divergence time between C. carbonarius and morphotype 1 is more recent than the divergence between C. denticulatus and C. carbonarius, due to the highly conserved character of this functional protein. Thus, considering the high degree of conservation of hemoglobins in vertebrates, and the differences observed in electrophoresis at alkaline pH and HPLC, we infer that C. carbonarius and morphotype 1 present a common branch. |
id |
UNSP_205a10234065c591ae9bbb8227b6e10a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/237653 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian TestudinidaeTestudinidaeHemoglobinElectrophoresisChelonoidisThe Brazilian Testudinidae family is widespread across South America. It includes Chelonoidis denticulatus, the largest tortoise in South America and Chelonoidis carbonarius, found mostly in the north and northwestern part of the continent. Using hemoglobin to identify species is cheaper than other methods such as DNA sequencing and can offer useful information, since the hemoglobin molecule is a well-preserved protein chain during the evolution of species. Thus, in order to establish a hemoglobin profile for the Brazilian Testudinidae C. denticulatus, C. carbonarius and morphotype 1, hemoglobin electrophoresis was performed at acid pH in phosphate agar and at alkaline pH in cellulose acetate, in order to visualize the specific fractions of each species. High performance liquid chromatography was used for the quantification of fractions. For an in-depth analysis and better detailing of the hemoglobin profile of the species, polypeptide chain electrophoresis was performed at acid and alkaline pH. We observed differences in the hemoglobin profiles of C. denticulatus in relation to C. carbonarius and morphotype 1, which suggests that this methodology, not common in taxonomic studies, can help determine relationships between species, since hemoglobins are proteins with well-preserved genes. We found differences in hemoglobin mobility between C. denticulatus, C. carbonarius and morphotype 1 in electrophoresis at alkaline pH, however, the behavior of globin chains was similar between the three groups. High performance liquid chromatography showed different retention times in the globin fractions of C. denticulatus and C. carbonarius, but not between C. carbonarius and morphotype 1, indicating that, possibly, the divergence time between C. carbonarius and morphotype 1 is more recent than the divergence between C. denticulatus and C. carbonarius, due to the highly conserved character of this functional protein. Thus, considering the high degree of conservation of hemoglobins in vertebrates, and the differences observed in electrophoresis at alkaline pH and HPLC, we infer that C. carbonarius and morphotype 1 present a common branch.Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), BrazilFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Scientific and Technological Research of Acre (FAPAC)Univ Fed Acre, Campus Floresta Cruzeiro do Sul, Cruzeiro Do Sul, AC, BrazilInst Nacl de Pesquisas da Amazonia, Dept Genet Conservacao & Biol Evolut, Manaus, Amazonas, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP, BrazilUniv Fed Oeste Bahia, Ctr Ciencias Biol & Saude, Campus Reitor Edgard Santos, Barreiras, BA, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilFunpec-editoraUniv Fed AcreInst Nacl de Pesquisas da AmazoniaUniversidade de São Paulo (USP)Univ Fed Oeste BahiaUniversidade Estadual Paulista (UNESP)Silva, T. L.Cardoso, V. L. O.Costa, N. R. A.Venancio, L. P. R.Pereira, L. R. [UNESP]Bonini-Domingos, C. R. [UNESP]2022-11-30T13:40:57Z2022-11-30T13:40:57Z2022-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.4238/gmr18977Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 21, n. 3, 9 p., 2022.16765680http://hdl.handle.net/11449/23765310.4238/gmr18977WOS:000836395300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Researchinfo:eu-repo/semantics/openAccess2022-11-30T13:40:57Zoai:repositorio.unesp.br:11449/237653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-11-30T13:40:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
title |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
spellingShingle |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae Silva, T. L. Testudinidae Hemoglobin Electrophoresis Chelonoidis |
title_short |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
title_full |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
title_fullStr |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
title_full_unstemmed |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
title_sort |
Hemoglobin protein profile as a parameter for taxonomic analysis in Brazilian Testudinidae |
author |
Silva, T. L. |
author_facet |
Silva, T. L. Cardoso, V. L. O. Costa, N. R. A. Venancio, L. P. R. Pereira, L. R. [UNESP] Bonini-Domingos, C. R. [UNESP] |
author_role |
author |
author2 |
Cardoso, V. L. O. Costa, N. R. A. Venancio, L. P. R. Pereira, L. R. [UNESP] Bonini-Domingos, C. R. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Acre Inst Nacl de Pesquisas da Amazonia Universidade de São Paulo (USP) Univ Fed Oeste Bahia Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, T. L. Cardoso, V. L. O. Costa, N. R. A. Venancio, L. P. R. Pereira, L. R. [UNESP] Bonini-Domingos, C. R. [UNESP] |
dc.subject.por.fl_str_mv |
Testudinidae Hemoglobin Electrophoresis Chelonoidis |
topic |
Testudinidae Hemoglobin Electrophoresis Chelonoidis |
description |
The Brazilian Testudinidae family is widespread across South America. It includes Chelonoidis denticulatus, the largest tortoise in South America and Chelonoidis carbonarius, found mostly in the north and northwestern part of the continent. Using hemoglobin to identify species is cheaper than other methods such as DNA sequencing and can offer useful information, since the hemoglobin molecule is a well-preserved protein chain during the evolution of species. Thus, in order to establish a hemoglobin profile for the Brazilian Testudinidae C. denticulatus, C. carbonarius and morphotype 1, hemoglobin electrophoresis was performed at acid pH in phosphate agar and at alkaline pH in cellulose acetate, in order to visualize the specific fractions of each species. High performance liquid chromatography was used for the quantification of fractions. For an in-depth analysis and better detailing of the hemoglobin profile of the species, polypeptide chain electrophoresis was performed at acid and alkaline pH. We observed differences in the hemoglobin profiles of C. denticulatus in relation to C. carbonarius and morphotype 1, which suggests that this methodology, not common in taxonomic studies, can help determine relationships between species, since hemoglobins are proteins with well-preserved genes. We found differences in hemoglobin mobility between C. denticulatus, C. carbonarius and morphotype 1 in electrophoresis at alkaline pH, however, the behavior of globin chains was similar between the three groups. High performance liquid chromatography showed different retention times in the globin fractions of C. denticulatus and C. carbonarius, but not between C. carbonarius and morphotype 1, indicating that, possibly, the divergence time between C. carbonarius and morphotype 1 is more recent than the divergence between C. denticulatus and C. carbonarius, due to the highly conserved character of this functional protein. Thus, considering the high degree of conservation of hemoglobins in vertebrates, and the differences observed in electrophoresis at alkaline pH and HPLC, we infer that C. carbonarius and morphotype 1 present a common branch. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:40:57Z 2022-11-30T13:40:57Z 2022-07-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/gmr18977 Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 21, n. 3, 9 p., 2022. 16765680 http://hdl.handle.net/11449/237653 10.4238/gmr18977 WOS:000836395300001 |
url |
http://dx.doi.org/10.4238/gmr18977 http://hdl.handle.net/11449/237653 |
identifier_str_mv |
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 21, n. 3, 9 p., 2022. 16765680 10.4238/gmr18977 WOS:000836395300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047139254730752 |