Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study

Detalhes bibliográficos
Autor(a) principal: Neto, Pedro Fraccarolli
Data de Publicação: 2019
Outros Autores: da Silva, Agnaldo Fernando Baldo, Moro, Evandro Bilha [UNESP], Pilarski, Fabiana [UNESP], de Freitas, Osvaldo, Mooney, Mark H., Paschoal, Jonas Augusto Rizzato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.foodres.2019.01.033
http://hdl.handle.net/11449/188654
Resumo: Due to the lack of regulated drugs for aquaculture, the present study considered specific issues relating to environmental and food safety aspects concerning the potential use of emamectin benzoate (EMA) in freshwater fishes such as tilapia (Oreochromis niloticus) – an important commercial fish species worldwide. The residual depletion of EMA (EMA-B 1a ) in fillet (muscle plus skin in natural proportions) of tilapia treated with a daily dose of 50 μg/kg BW during seven consecutive days was evaluated. To facilitate this, analytical methods for quantitation of EMA in fish feed and in fish fillet employing LC-MS/MS were developed and validated. To eliminate the risk of EMA leaching from feed into the aquatic environment during fish medication via oral administration, a promising procedure for drug incorporation into feed involving the coating of feed pellets with ethyl cellulose polymer containing EMA was evaluated. The medicated feed exhibited good homogeneity (CV < 2.1%) with negligible EMA release (< LOQ) when the medicated feed remained in the water for up to 20 min. Depletion study analysis revealed the highest EMA concentration obtained in fish fillet to be 13.3 ng/g. Therefore, under the employed rearing conditions of this study, the obtained results did not evidence requirement for a minimum withdrawal period to be proposed considering the maximum residue limit of 100 μg/g for fish muscle. In response to the well-recognized demands and need for new alternative veterinary drugs for use within aquaculture, this study offers impetus for consideration of EMA use in tilapia taking into account environmental contamination and food safety issues.
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spelling Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion studyAquacultureEmamectin benzoateFood safetyResidual depletionTilapiaVeterinary drug residuesWithdrawal periodDue to the lack of regulated drugs for aquaculture, the present study considered specific issues relating to environmental and food safety aspects concerning the potential use of emamectin benzoate (EMA) in freshwater fishes such as tilapia (Oreochromis niloticus) – an important commercial fish species worldwide. The residual depletion of EMA (EMA-B 1a ) in fillet (muscle plus skin in natural proportions) of tilapia treated with a daily dose of 50 μg/kg BW during seven consecutive days was evaluated. To facilitate this, analytical methods for quantitation of EMA in fish feed and in fish fillet employing LC-MS/MS were developed and validated. To eliminate the risk of EMA leaching from feed into the aquatic environment during fish medication via oral administration, a promising procedure for drug incorporation into feed involving the coating of feed pellets with ethyl cellulose polymer containing EMA was evaluated. The medicated feed exhibited good homogeneity (CV < 2.1%) with negligible EMA release (< LOQ) when the medicated feed remained in the water for up to 20 min. Depletion study analysis revealed the highest EMA concentration obtained in fish fillet to be 13.3 ng/g. Therefore, under the employed rearing conditions of this study, the obtained results did not evidence requirement for a minimum withdrawal period to be proposed considering the maximum residue limit of 100 μg/g for fish muscle. In response to the well-recognized demands and need for new alternative veterinary drugs for use within aquaculture, this study offers impetus for consideration of EMA use in tilapia taking into account environmental contamination and food safety issues.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Física e Química Universidade de São Paulo (USP)Centro de Aquacultura da Unesp Universidade do Estado de São Paulo (Unesp)Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Ciências Farmacêuticas Universidade de São Paulo (USP)Institute for Global Food Security School of Biological Sciences Queen's University BelfastCentro de Aquacultura da Unesp Universidade do Estado de São Paulo (Unesp)FAPESP: 2012/18334-0FAPESP: 2014/50755-0Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Queen's University BelfastNeto, Pedro Fraccarollida Silva, Agnaldo Fernando BaldoMoro, Evandro Bilha [UNESP]Pilarski, Fabiana [UNESP]de Freitas, OsvaldoMooney, Mark H.Paschoal, Jonas Augusto Rizzato2019-10-06T16:15:00Z2019-10-06T16:15:00Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article524-529http://dx.doi.org/10.1016/j.foodres.2019.01.033Food Research International, v. 119, p. 524-529.1873-71450963-9969http://hdl.handle.net/11449/18865410.1016/j.foodres.2019.01.0332-s2.0-85060350844Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Research Internationalinfo:eu-repo/semantics/openAccess2024-04-09T15:43:17Zoai:repositorio.unesp.br:11449/188654Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:17:30.676351Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
title Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
spellingShingle Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
Neto, Pedro Fraccarolli
Aquaculture
Emamectin benzoate
Food safety
Residual depletion
Tilapia
Veterinary drug residues
Withdrawal period
title_short Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
title_full Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
title_fullStr Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
title_full_unstemmed Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
title_sort Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study
author Neto, Pedro Fraccarolli
author_facet Neto, Pedro Fraccarolli
da Silva, Agnaldo Fernando Baldo
Moro, Evandro Bilha [UNESP]
Pilarski, Fabiana [UNESP]
de Freitas, Osvaldo
Mooney, Mark H.
Paschoal, Jonas Augusto Rizzato
author_role author
author2 da Silva, Agnaldo Fernando Baldo
Moro, Evandro Bilha [UNESP]
Pilarski, Fabiana [UNESP]
de Freitas, Osvaldo
Mooney, Mark H.
Paschoal, Jonas Augusto Rizzato
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Queen's University Belfast
dc.contributor.author.fl_str_mv Neto, Pedro Fraccarolli
da Silva, Agnaldo Fernando Baldo
Moro, Evandro Bilha [UNESP]
Pilarski, Fabiana [UNESP]
de Freitas, Osvaldo
Mooney, Mark H.
Paschoal, Jonas Augusto Rizzato
dc.subject.por.fl_str_mv Aquaculture
Emamectin benzoate
Food safety
Residual depletion
Tilapia
Veterinary drug residues
Withdrawal period
topic Aquaculture
Emamectin benzoate
Food safety
Residual depletion
Tilapia
Veterinary drug residues
Withdrawal period
description Due to the lack of regulated drugs for aquaculture, the present study considered specific issues relating to environmental and food safety aspects concerning the potential use of emamectin benzoate (EMA) in freshwater fishes such as tilapia (Oreochromis niloticus) – an important commercial fish species worldwide. The residual depletion of EMA (EMA-B 1a ) in fillet (muscle plus skin in natural proportions) of tilapia treated with a daily dose of 50 μg/kg BW during seven consecutive days was evaluated. To facilitate this, analytical methods for quantitation of EMA in fish feed and in fish fillet employing LC-MS/MS were developed and validated. To eliminate the risk of EMA leaching from feed into the aquatic environment during fish medication via oral administration, a promising procedure for drug incorporation into feed involving the coating of feed pellets with ethyl cellulose polymer containing EMA was evaluated. The medicated feed exhibited good homogeneity (CV < 2.1%) with negligible EMA release (< LOQ) when the medicated feed remained in the water for up to 20 min. Depletion study analysis revealed the highest EMA concentration obtained in fish fillet to be 13.3 ng/g. Therefore, under the employed rearing conditions of this study, the obtained results did not evidence requirement for a minimum withdrawal period to be proposed considering the maximum residue limit of 100 μg/g for fish muscle. In response to the well-recognized demands and need for new alternative veterinary drugs for use within aquaculture, this study offers impetus for consideration of EMA use in tilapia taking into account environmental contamination and food safety issues.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:15:00Z
2019-10-06T16:15:00Z
2019-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.foodres.2019.01.033
Food Research International, v. 119, p. 524-529.
1873-7145
0963-9969
http://hdl.handle.net/11449/188654
10.1016/j.foodres.2019.01.033
2-s2.0-85060350844
url http://dx.doi.org/10.1016/j.foodres.2019.01.033
http://hdl.handle.net/11449/188654
identifier_str_mv Food Research International, v. 119, p. 524-529.
1873-7145
0963-9969
10.1016/j.foodres.2019.01.033
2-s2.0-85060350844
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Research International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 524-529
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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