Aplicação do sistema de biosusceptometria AC para monitoramento de nanopartículas magnéticas na circulação sanguínea

• Autor(a) principal: Próspero, André Gonçalves [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/131881; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/06-11-2015/000848395.pdf
• topic: Biosusceptometria de Corrente Alternada; Coração - Fisiologia; Nanopartículas; Biomagnetismo; Sangue - Circulação; Alternated Current Biosusceptometry

In the Biomagnetic research field, several methods have reported to study physiological parameters through the detection of magnetic fields generated by biological electrical currents or magnetic marker/tracers. Among such techniques, the Alternating Current Biosusceptometry (AC Biosusceptometry or ACB) system has been developed and refined with the main purpose of detecting and monitoring magnetic materials in biological systems (mainly in the gastrointestinal tract so far). With a pair of gradiometric pickup coils, the ACB system is able to obtain data related to local biological properties and also to study mechanical and physiological parameters, such as gastric emptying and contraction activity. This work presents an unprecedented study that applies the ACB system, associated to magnetic nanoparticles, as a new tool for preclinical characterization of magnetic nanoparticles. In vivo Experiments were performed in rat models to quantify the magnetic nanoparticles half-life in predefined time intervals, the particles arrival time in the animals heart and also the nanoparticles biodistribution. As an additional test, cardiovascular parameters were acquired during the particle intravenous administration, since these parameters directly influence the material half-life and biodistribution. The system presented good performance in all tests, with excellent reproducibility, sensitivity and temporal resolution. Also, these results suggest the ACB using as valuable evaluation method for magnetic nanoparticles preclinical characterization, in parameters as biodistribution and half-life in the bloodstream, since transient changes in mean arterial pressure and heart rate were found due to the magnetic nanoparticles intravenous administration. This work includes the development and characterization of a new low cost physics instrumentation, to subsidize circulatory system research, in small animals, and new nanostructured materials applications ...