Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2010.12.005 http://hdl.handle.net/11449/42590 |
Resumo: | Pain is the most conspicuous symptom observed in patients wounded by stingrays, and skin necrosis is common in accidents by freshwater stingrays. The extract from the stinger integumentary tissue of Potamotrygon falkneri containing toxic components (venom) was tested for its ability to induce histopathological changes in the dorsal skin of mice at different times. 3-6 h after injection, foci of necrosis in isolated basal epidermal cells were observed. Full coagulative necrosis of the skin, subcutaneous tissue and skeletal muscle was evident as soon as 24 h after venom exposure, with a clear demarcation from the normal skin. After 48 h, round collections of necrotic cells start to coalesce originating extensive skin necrotic plaques that detach from viable tissue after 72-96 h. Inflammatory infiltrate was observed after 6 h, but was always mild. Acute vascular thrombosis was rare, and hemorrhage was not present at any time. Superficial bacterial infection was present in two of the examined cases. In conclusion, the venom of P. falkneri is responsible for the development of an early necrosis with mild inflammatory reaction, probably due to direct action of the venom. The severe local damage is probably worsened by the mechanical trauma caused by the stinger. (c) 2010 Elsevier Ltd. All rights reserved. |
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Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingraysStingraysPotamotrygonVenomToxinDermonecrosisHistopathologyPain is the most conspicuous symptom observed in patients wounded by stingrays, and skin necrosis is common in accidents by freshwater stingrays. The extract from the stinger integumentary tissue of Potamotrygon falkneri containing toxic components (venom) was tested for its ability to induce histopathological changes in the dorsal skin of mice at different times. 3-6 h after injection, foci of necrosis in isolated basal epidermal cells were observed. Full coagulative necrosis of the skin, subcutaneous tissue and skeletal muscle was evident as soon as 24 h after venom exposure, with a clear demarcation from the normal skin. After 48 h, round collections of necrotic cells start to coalesce originating extensive skin necrotic plaques that detach from viable tissue after 72-96 h. Inflammatory infiltrate was observed after 6 h, but was always mild. Acute vascular thrombosis was rare, and hemorrhage was not present at any time. Superficial bacterial infection was present in two of the examined cases. In conclusion, the venom of P. falkneri is responsible for the development of an early necrosis with mild inflammatory reaction, probably due to direct action of the venom. The severe local damage is probably worsened by the mechanical trauma caused by the stinger. (c) 2010 Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Inst Butantan, Lab Imunopatol, BR-05503900 São Paulo, BrazilInst Butantan, Lab Biol Celular, BR-05503900 São Paulo, BrazilUniv São Paulo, Inst Coracao InCor, Fac Med, BR-05403000 São Paulo, BrazilBrazil UNESP, Inst Biociencias, UNESP, BR-18618000 Botucatu, SP, BrazilBrazil UNESP, Inst Biociencias, UNESP, BR-18618000 Botucatu, SP, BrazilFAPESP: 07/55272-4CNPq: 304800/2007-4CNPq: 142985/2005-8CNPq: 307029/2009-3CNPq: 307247/2007-4Pergamon-Elsevier B.V. LtdInstituto ButantanUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Antoniazzi, Marta M.Benvenuti, Luiz A.Lira, Marcela S.Jared, Simone G. S.Garrone Neto, Domingos [UNESP]Jared, CarlosBarbaro, Katia C.2014-05-20T15:34:35Z2014-05-20T15:34:35Z2011-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article297-303application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2010.12.005Toxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 297-303, 2011.0041-0101http://hdl.handle.net/11449/4259010.1016/j.toxicon.2010.12.005WOS:000287629400013WOS000287629400013.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicon2.3520,692info:eu-repo/semantics/openAccess2023-11-25T06:17:55Zoai:repositorio.unesp.br:11449/42590Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:17:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
title |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
spellingShingle |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays Antoniazzi, Marta M. Stingrays Potamotrygon Venom Toxin Dermonecrosis Histopathology |
title_short |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
title_full |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
title_fullStr |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
title_full_unstemmed |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
title_sort |
Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays |
author |
Antoniazzi, Marta M. |
author_facet |
Antoniazzi, Marta M. Benvenuti, Luiz A. Lira, Marcela S. Jared, Simone G. S. Garrone Neto, Domingos [UNESP] Jared, Carlos Barbaro, Katia C. |
author_role |
author |
author2 |
Benvenuti, Luiz A. Lira, Marcela S. Jared, Simone G. S. Garrone Neto, Domingos [UNESP] Jared, Carlos Barbaro, Katia C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Butantan Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Antoniazzi, Marta M. Benvenuti, Luiz A. Lira, Marcela S. Jared, Simone G. S. Garrone Neto, Domingos [UNESP] Jared, Carlos Barbaro, Katia C. |
dc.subject.por.fl_str_mv |
Stingrays Potamotrygon Venom Toxin Dermonecrosis Histopathology |
topic |
Stingrays Potamotrygon Venom Toxin Dermonecrosis Histopathology |
description |
Pain is the most conspicuous symptom observed in patients wounded by stingrays, and skin necrosis is common in accidents by freshwater stingrays. The extract from the stinger integumentary tissue of Potamotrygon falkneri containing toxic components (venom) was tested for its ability to induce histopathological changes in the dorsal skin of mice at different times. 3-6 h after injection, foci of necrosis in isolated basal epidermal cells were observed. Full coagulative necrosis of the skin, subcutaneous tissue and skeletal muscle was evident as soon as 24 h after venom exposure, with a clear demarcation from the normal skin. After 48 h, round collections of necrotic cells start to coalesce originating extensive skin necrotic plaques that detach from viable tissue after 72-96 h. Inflammatory infiltrate was observed after 6 h, but was always mild. Acute vascular thrombosis was rare, and hemorrhage was not present at any time. Superficial bacterial infection was present in two of the examined cases. In conclusion, the venom of P. falkneri is responsible for the development of an early necrosis with mild inflammatory reaction, probably due to direct action of the venom. The severe local damage is probably worsened by the mechanical trauma caused by the stinger. (c) 2010 Elsevier Ltd. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 2014-05-20T15:34:35Z 2014-05-20T15:34:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2010.12.005 Toxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 297-303, 2011. 0041-0101 http://hdl.handle.net/11449/42590 10.1016/j.toxicon.2010.12.005 WOS:000287629400013 WOS000287629400013.pdf |
url |
http://dx.doi.org/10.1016/j.toxicon.2010.12.005 http://hdl.handle.net/11449/42590 |
identifier_str_mv |
Toxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 297-303, 2011. 0041-0101 10.1016/j.toxicon.2010.12.005 WOS:000287629400013 WOS000287629400013.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon 2.352 0,692 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
297-303 application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046656574226432 |