Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2019.105071 http://hdl.handle.net/11449/187874 |
Resumo: | The blood-sucking insects of the subfamily Triatominae are vectors of Chagas disease, the most impairing protozoan parasitic infection in Latin America. Among the five tribes known in the subfamily, one of the least studied is Cavernicolini. It has only two species within Cavernicola genus (Barber, 1937), little is known about the biochemistry of the species of this genus, therefore, using MALDI-TOF MS we provide a better understanding of the two species and differentiates them. The distinction was made by the different spectral profile of the species, where C. lenti presents unique signals in many regions, while the C. pilosa shows high-intensity signals and m/z in high bands. The application of digital mass spectrometry combined with chemometric methods was able to accurately distinguish two species of the genus Cavernicola. |
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Repositório Institucional da UNESP |
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spelling |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometryCavernicolaMALDI TOFMass spectrometryThe blood-sucking insects of the subfamily Triatominae are vectors of Chagas disease, the most impairing protozoan parasitic infection in Latin America. Among the five tribes known in the subfamily, one of the least studied is Cavernicolini. It has only two species within Cavernicola genus (Barber, 1937), little is known about the biochemistry of the species of this genus, therefore, using MALDI-TOF MS we provide a better understanding of the two species and differentiates them. The distinction was made by the different spectral profile of the species, where C. lenti presents unique signals in many regions, while the C. pilosa shows high-intensity signals and m/z in high bands. The application of digital mass spectrometry combined with chemometric methods was able to accurately distinguish two species of the genus Cavernicola.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Faculdade de Ciencias Farmacêuticas Universidade Estadual Paulista “Julio de Mesquita Filho” (UNESP)Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de QuímicaLaboratorio Nacional e Internacional de Referência em Taxonomia de Triatomíneos Instituto Oswaldo Cruz FIOCRUZInstituto de Biologia Universidade Estadual de Campinas (UNICAMP)Faculdade de Ciencias Farmacêuticas Universidade Estadual Paulista “Julio de Mesquita Filho” (UNESP)Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de QuímicaUniversidade Estadual Paulista (Unesp)FIOCRUZUniversidade Estadual de Campinas (UNICAMP)dos Santos Souza, Éder [UNESP]Fernandes, Richard Perosa [UNESP]Galvão, Cleberde Paiva, Vinícius Fernandesda Rosa, João Aristeu [UNESP]2019-10-06T15:49:56Z2019-10-06T15:49:56Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2019.105071Acta Tropica, v. 198.1873-62540001-706Xhttp://hdl.handle.net/11449/18787410.1016/j.actatropica.2019.1050712-s2.0-85069155734Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2021-10-23T00:57:26Zoai:repositorio.unesp.br:11449/187874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:06:29.870457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
title |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
spellingShingle |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry dos Santos Souza, Éder [UNESP] Cavernicola MALDI TOF Mass spectrometry |
title_short |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
title_full |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
title_fullStr |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
title_full_unstemmed |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
title_sort |
Distinguishing two species of Cavernicola (Hemiptera, Reduviidae, Triatominae) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
author |
dos Santos Souza, Éder [UNESP] |
author_facet |
dos Santos Souza, Éder [UNESP] Fernandes, Richard Perosa [UNESP] Galvão, Cleber de Paiva, Vinícius Fernandes da Rosa, João Aristeu [UNESP] |
author_role |
author |
author2 |
Fernandes, Richard Perosa [UNESP] Galvão, Cleber de Paiva, Vinícius Fernandes da Rosa, João Aristeu [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) FIOCRUZ Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
dos Santos Souza, Éder [UNESP] Fernandes, Richard Perosa [UNESP] Galvão, Cleber de Paiva, Vinícius Fernandes da Rosa, João Aristeu [UNESP] |
dc.subject.por.fl_str_mv |
Cavernicola MALDI TOF Mass spectrometry |
topic |
Cavernicola MALDI TOF Mass spectrometry |
description |
The blood-sucking insects of the subfamily Triatominae are vectors of Chagas disease, the most impairing protozoan parasitic infection in Latin America. Among the five tribes known in the subfamily, one of the least studied is Cavernicolini. It has only two species within Cavernicola genus (Barber, 1937), little is known about the biochemistry of the species of this genus, therefore, using MALDI-TOF MS we provide a better understanding of the two species and differentiates them. The distinction was made by the different spectral profile of the species, where C. lenti presents unique signals in many regions, while the C. pilosa shows high-intensity signals and m/z in high bands. The application of digital mass spectrometry combined with chemometric methods was able to accurately distinguish two species of the genus Cavernicola. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:49:56Z 2019-10-06T15:49:56Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2019.105071 Acta Tropica, v. 198. 1873-6254 0001-706X http://hdl.handle.net/11449/187874 10.1016/j.actatropica.2019.105071 2-s2.0-85069155734 |
url |
http://dx.doi.org/10.1016/j.actatropica.2019.105071 http://hdl.handle.net/11449/187874 |
identifier_str_mv |
Acta Tropica, v. 198. 1873-6254 0001-706X 10.1016/j.actatropica.2019.105071 2-s2.0-85069155734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128462722957312 |