Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support

Detalhes bibliográficos
Autor(a) principal: Aquaroni, Nayara Ap. Simei
Data de Publicação: 2020
Outros Autores: Nakahata, Douglas H., Lazarini, Silmara C., Resende, Flávia A., Cândido, Amanda L.P., da Silva Barud, Hernane, Claro, Amanda Maria, de Carvalho, João Ernesto, Ribeiro, Camila M. [UNESP], Pavan, Fernando R. [UNESP], Lustri, Bruna C. [UNESP], Ribeiro, Tamara Renata Machado [UNESP], Moreira, Cristiano G. [UNESP], Cândido, Tuany Zambroti, Lima, Carmen Silvia Passos, Ruiz, Ana Lúcia T.G., Corbi, Pedro P., Lustri, Wilton R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jinorgbio.2020.111247
http://hdl.handle.net/11449/202075
Resumo: The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.
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spelling Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support4-Aminobenzoic acidAntibacterial activityAntiproliferative activityDNA interactionDrug release systemMutagenic activitySilver complexThe aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)University of Araraquara – UNIARAInstitute of Chemistry University of Campinas – UNICAMPFaculty of Pharmaceutical Sciences University of Campinas – UNICAMPSão Paulo State University – UNESP School of Pharmaceutical SciencesFaculty of Medical Sciences University of Campinas – UNICAMPSão Paulo State University – UNESP School of Pharmaceutical SciencesFAPESP: , 2015/09833-0CAPES: 001FAPESP: 019/03049-7FAPESP: 13/07276-1FAPESP: 2014/06779-2FAPESP: 2015/20882-3FAPESP: 2016/07729-4FAPESP: 2017/16278-9FAPESP: 2017/19243-1FAPESP: 2018/00163-0FAPESP: 2018/02344-2FAPESP: 2018/12062-4FAPESP: 2018/12590-0FAPESP: 2018/25512-8CNPq: 300968/2016-7CNPq: 407822/2018University of Araraquara – UNIARAUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Aquaroni, Nayara Ap. SimeiNakahata, Douglas H.Lazarini, Silmara C.Resende, Flávia A.Cândido, Amanda L.P.da Silva Barud, HernaneClaro, Amanda Mariade Carvalho, João ErnestoRibeiro, Camila M. [UNESP]Pavan, Fernando R. [UNESP]Lustri, Bruna C. [UNESP]Ribeiro, Tamara Renata Machado [UNESP]Moreira, Cristiano G. [UNESP]Cândido, Tuany ZambrotiLima, Carmen Silvia PassosRuiz, Ana Lúcia T.G.Corbi, Pedro P.Lustri, Wilton R.2020-12-12T02:49:12Z2020-12-12T02:49:12Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jinorgbio.2020.111247Journal of Inorganic Biochemistry, v. 212.1873-33440162-0134http://hdl.handle.net/11449/20207510.1016/j.jinorgbio.2020.1112472-s2.0-85090406382Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Inorganic Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T05:09:16Zoai:repositorio.unesp.br:11449/202075Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:09:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
title Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
spellingShingle Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
Aquaroni, Nayara Ap. Simei
4-Aminobenzoic acid
Antibacterial activity
Antiproliferative activity
DNA interaction
Drug release system
Mutagenic activity
Silver complex
title_short Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
title_full Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
title_fullStr Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
title_full_unstemmed Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
title_sort Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
author Aquaroni, Nayara Ap. Simei
author_facet Aquaroni, Nayara Ap. Simei
Nakahata, Douglas H.
Lazarini, Silmara C.
Resende, Flávia A.
Cândido, Amanda L.P.
da Silva Barud, Hernane
Claro, Amanda Maria
de Carvalho, João Ernesto
Ribeiro, Camila M. [UNESP]
Pavan, Fernando R. [UNESP]
Lustri, Bruna C. [UNESP]
Ribeiro, Tamara Renata Machado [UNESP]
Moreira, Cristiano G. [UNESP]
Cândido, Tuany Zambroti
Lima, Carmen Silvia Passos
Ruiz, Ana Lúcia T.G.
Corbi, Pedro P.
Lustri, Wilton R.
author_role author
author2 Nakahata, Douglas H.
Lazarini, Silmara C.
Resende, Flávia A.
Cândido, Amanda L.P.
da Silva Barud, Hernane
Claro, Amanda Maria
de Carvalho, João Ernesto
Ribeiro, Camila M. [UNESP]
Pavan, Fernando R. [UNESP]
Lustri, Bruna C. [UNESP]
Ribeiro, Tamara Renata Machado [UNESP]
Moreira, Cristiano G. [UNESP]
Cândido, Tuany Zambroti
Lima, Carmen Silvia Passos
Ruiz, Ana Lúcia T.G.
Corbi, Pedro P.
Lustri, Wilton R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Araraquara – UNIARA
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Aquaroni, Nayara Ap. Simei
Nakahata, Douglas H.
Lazarini, Silmara C.
Resende, Flávia A.
Cândido, Amanda L.P.
da Silva Barud, Hernane
Claro, Amanda Maria
de Carvalho, João Ernesto
Ribeiro, Camila M. [UNESP]
Pavan, Fernando R. [UNESP]
Lustri, Bruna C. [UNESP]
Ribeiro, Tamara Renata Machado [UNESP]
Moreira, Cristiano G. [UNESP]
Cândido, Tuany Zambroti
Lima, Carmen Silvia Passos
Ruiz, Ana Lúcia T.G.
Corbi, Pedro P.
Lustri, Wilton R.
dc.subject.por.fl_str_mv 4-Aminobenzoic acid
Antibacterial activity
Antiproliferative activity
DNA interaction
Drug release system
Mutagenic activity
Silver complex
topic 4-Aminobenzoic acid
Antibacterial activity
Antiproliferative activity
DNA interaction
Drug release system
Mutagenic activity
Silver complex
description The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:49:12Z
2020-12-12T02:49:12Z
2020-11-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jinorgbio.2020.111247
Journal of Inorganic Biochemistry, v. 212.
1873-3344
0162-0134
http://hdl.handle.net/11449/202075
10.1016/j.jinorgbio.2020.111247
2-s2.0-85090406382
url http://dx.doi.org/10.1016/j.jinorgbio.2020.111247
http://hdl.handle.net/11449/202075
identifier_str_mv Journal of Inorganic Biochemistry, v. 212.
1873-3344
0162-0134
10.1016/j.jinorgbio.2020.111247
2-s2.0-85090406382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Inorganic Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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