Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jinorgbio.2020.111247 http://hdl.handle.net/11449/202075 |
Resumo: | The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer. |
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Repositório Institucional da UNESP |
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Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support4-Aminobenzoic acidAntibacterial activityAntiproliferative activityDNA interactionDrug release systemMutagenic activitySilver complexThe aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)University of Araraquara – UNIARAInstitute of Chemistry University of Campinas – UNICAMPFaculty of Pharmaceutical Sciences University of Campinas – UNICAMPSão Paulo State University – UNESP School of Pharmaceutical SciencesFaculty of Medical Sciences University of Campinas – UNICAMPSão Paulo State University – UNESP School of Pharmaceutical SciencesFAPESP: , 2015/09833-0CAPES: 001FAPESP: 019/03049-7FAPESP: 13/07276-1FAPESP: 2014/06779-2FAPESP: 2015/20882-3FAPESP: 2016/07729-4FAPESP: 2017/16278-9FAPESP: 2017/19243-1FAPESP: 2018/00163-0FAPESP: 2018/02344-2FAPESP: 2018/12062-4FAPESP: 2018/12590-0FAPESP: 2018/25512-8CNPq: 300968/2016-7CNPq: 407822/2018University of Araraquara – UNIARAUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Aquaroni, Nayara Ap. SimeiNakahata, Douglas H.Lazarini, Silmara C.Resende, Flávia A.Cândido, Amanda L.P.da Silva Barud, HernaneClaro, Amanda Mariade Carvalho, João ErnestoRibeiro, Camila M. [UNESP]Pavan, Fernando R. [UNESP]Lustri, Bruna C. [UNESP]Ribeiro, Tamara Renata Machado [UNESP]Moreira, Cristiano G. [UNESP]Cândido, Tuany ZambrotiLima, Carmen Silvia PassosRuiz, Ana Lúcia T.G.Corbi, Pedro P.Lustri, Wilton R.2020-12-12T02:49:12Z2020-12-12T02:49:12Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jinorgbio.2020.111247Journal of Inorganic Biochemistry, v. 212.1873-33440162-0134http://hdl.handle.net/11449/20207510.1016/j.jinorgbio.2020.1112472-s2.0-85090406382Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Inorganic Biochemistryinfo:eu-repo/semantics/openAccess2024-06-24T13:08:01Zoai:repositorio.unesp.br:11449/202075Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:30:23.261201Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
title |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
spellingShingle |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support Aquaroni, Nayara Ap. Simei 4-Aminobenzoic acid Antibacterial activity Antiproliferative activity DNA interaction Drug release system Mutagenic activity Silver complex |
title_short |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
title_full |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
title_fullStr |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
title_full_unstemmed |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
title_sort |
Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support |
author |
Aquaroni, Nayara Ap. Simei |
author_facet |
Aquaroni, Nayara Ap. Simei Nakahata, Douglas H. Lazarini, Silmara C. Resende, Flávia A. Cândido, Amanda L.P. da Silva Barud, Hernane Claro, Amanda Maria de Carvalho, João Ernesto Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Lustri, Bruna C. [UNESP] Ribeiro, Tamara Renata Machado [UNESP] Moreira, Cristiano G. [UNESP] Cândido, Tuany Zambroti Lima, Carmen Silvia Passos Ruiz, Ana Lúcia T.G. Corbi, Pedro P. Lustri, Wilton R. |
author_role |
author |
author2 |
Nakahata, Douglas H. Lazarini, Silmara C. Resende, Flávia A. Cândido, Amanda L.P. da Silva Barud, Hernane Claro, Amanda Maria de Carvalho, João Ernesto Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Lustri, Bruna C. [UNESP] Ribeiro, Tamara Renata Machado [UNESP] Moreira, Cristiano G. [UNESP] Cândido, Tuany Zambroti Lima, Carmen Silvia Passos Ruiz, Ana Lúcia T.G. Corbi, Pedro P. Lustri, Wilton R. |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Araraquara – UNIARA Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Aquaroni, Nayara Ap. Simei Nakahata, Douglas H. Lazarini, Silmara C. Resende, Flávia A. Cândido, Amanda L.P. da Silva Barud, Hernane Claro, Amanda Maria de Carvalho, João Ernesto Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Lustri, Bruna C. [UNESP] Ribeiro, Tamara Renata Machado [UNESP] Moreira, Cristiano G. [UNESP] Cândido, Tuany Zambroti Lima, Carmen Silvia Passos Ruiz, Ana Lúcia T.G. Corbi, Pedro P. Lustri, Wilton R. |
dc.subject.por.fl_str_mv |
4-Aminobenzoic acid Antibacterial activity Antiproliferative activity DNA interaction Drug release system Mutagenic activity Silver complex |
topic |
4-Aminobenzoic acid Antibacterial activity Antiproliferative activity DNA interaction Drug release system Mutagenic activity Silver complex |
description |
The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:49:12Z 2020-12-12T02:49:12Z 2020-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jinorgbio.2020.111247 Journal of Inorganic Biochemistry, v. 212. 1873-3344 0162-0134 http://hdl.handle.net/11449/202075 10.1016/j.jinorgbio.2020.111247 2-s2.0-85090406382 |
url |
http://dx.doi.org/10.1016/j.jinorgbio.2020.111247 http://hdl.handle.net/11449/202075 |
identifier_str_mv |
Journal of Inorganic Biochemistry, v. 212. 1873-3344 0162-0134 10.1016/j.jinorgbio.2020.111247 2-s2.0-85090406382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Inorganic Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129211222720512 |