Agaricus blazei as a Substrate for the Production of beta-1,3-Glucanase by Trichoderma harzianum Rifai

• Autor(a) principal: Carneiro, Andreia A. J. [UNESP]
• Data de Publicação: 2011
• Outros Autores: Giese, Ellen C., Barbosa, Aneli M., Gomes, Eleni [UNESP], Da Silva, Roberto [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/195980
• topic: beta-1,3-glucanase; medicinal mushrooms; Trichoderma harzianum Rifai; response surface methodology

Extracellular beta-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2(2)-central composite factorial design was developed using the time of culture (x(1)/day) and Agaricus blazei extract concentration (x(2)/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of beta-1,3-glucanase, and the maximum beta-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The beta-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific beta-1,3-glucanase by Trichoderma harzianum Rifai.