Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai

• Autor(a) principal: Carneiro, Andréia A.J. [UNESP]
• Data de Publicação: 2011
• Outros Autores: Giese, Ellen C., Barbosa, Aneli M., Gomes, Eleni [UNESP], Da Silva, Roberto [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hrcak.srce.hr/file/105769; http://hdl.handle.net/11449/72540
• topic: β-1,3-glucanase; Medicinal mushrooms; Response surface methodology; Trichoderma harzianum Rifai; Agaricus; Agaricus blazei; Agaricus brasiliensis; Basidiomycota; Hypocrea lixii

Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 22-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract concentration (x2/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai.