Type IV secretion system is not involved in infection process in citrus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/763575 http://hdl.handle.net/11449/131011 |
Resumo: | The type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Xanthomonas citri subsp. citri contains two copies of the T4SS, one in the chromosome and the other is plasmid-encoded. To understand the conditions that induce expression of the T4SS in Xcc, we analyzed, in vitro and in planta, the expression of 18 ORFs from the T4SS and 7 hypothetical flanking genes by RT-qPCR. As a positive control, we also evaluated the expression of 29 ORFs from the type III secretion system (T3SS), since these genes are known to be expressed during plant infection condition, but not necessarily in standard culture medium. From the 29 T3SS genes analyzed by qPCR, only hrpA was downregulated at 72 h after inoculation. All genes associated with the T4SS were downregulated on Citrus leaves 72 h after inoculation. Our results showed that unlike the T3SS, the T4SS is not induced during the infection process. |
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Repositório Institucional da UNESP |
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spelling |
Type IV secretion system is not involved in infection process in citrusThe type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Xanthomonas citri subsp. citri contains two copies of the T4SS, one in the chromosome and the other is plasmid-encoded. To understand the conditions that induce expression of the T4SS in Xcc, we analyzed, in vitro and in planta, the expression of 18 ORFs from the T4SS and 7 hypothetical flanking genes by RT-qPCR. As a positive control, we also evaluated the expression of 29 ORFs from the type III secretion system (T3SS), since these genes are known to be expressed during plant infection condition, but not necessarily in standard culture medium. From the 29 T3SS genes analyzed by qPCR, only hrpA was downregulated at 72 h after inoculation. All genes associated with the T4SS were downregulated on Citrus leaves 72 h after inoculation. Our results showed that unlike the T3SS, the T4SS is not induced during the infection process.Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Departamento de Tecnologia, Universidade Estadual Paulista (UNESP), 14.884-900 Jaboticabal, SP, Brazil.Departamento de Engenharia Florestal, Faculdade de Ciências Agrárias, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 39.100-000 Diamantina, MG, Brazil.Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológicas (ICEB) and Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, 35.400-000 Ouro Preto, MG, Brazil.Embrapa Informática Agropecuária, CNPTIA-EMBRAPA, Campinas, SP, Brazil.Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Departamento de Tecnologia, Universidade Estadual Paulista (UNESP), 14.884-900 Jaboticabal, SP, Brazil.International Journal Of MicrobiologyUniversidade Estadual Paulista (Unesp)Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Universidade Federal de Ouro Preto (UFOP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Jacob, Tiago Rinaldi [UNESP]Laia, Marcelo Luiz deMoreira, Leandro MarcioGonçalves, Janaína FernandesCarvalho, Flavia Maria de SouzaFerro, Maria Inês Tiraboschi [UNESP]Ferro, Jesus Aparecido [UNESP]2015-12-07T15:30:49Z2015-12-07T15:30:49Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2014/763575International Journal Of Microbiology, v. 2014, 2014.1687-918Xhttp://hdl.handle.net/11449/13101110.1155/2014/763575PMC3953393.pdf0147241723612464758720848238405924707292PMC3953393PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2024-06-07T15:32:22Zoai:repositorio.unesp.br:11449/131011Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:13:00.996985Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Type IV secretion system is not involved in infection process in citrus |
title |
Type IV secretion system is not involved in infection process in citrus |
spellingShingle |
Type IV secretion system is not involved in infection process in citrus Jacob, Tiago Rinaldi [UNESP] |
title_short |
Type IV secretion system is not involved in infection process in citrus |
title_full |
Type IV secretion system is not involved in infection process in citrus |
title_fullStr |
Type IV secretion system is not involved in infection process in citrus |
title_full_unstemmed |
Type IV secretion system is not involved in infection process in citrus |
title_sort |
Type IV secretion system is not involved in infection process in citrus |
author |
Jacob, Tiago Rinaldi [UNESP] |
author_facet |
Jacob, Tiago Rinaldi [UNESP] Laia, Marcelo Luiz de Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Carvalho, Flavia Maria de Souza Ferro, Maria Inês Tiraboschi [UNESP] Ferro, Jesus Aparecido [UNESP] |
author_role |
author |
author2 |
Laia, Marcelo Luiz de Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Carvalho, Flavia Maria de Souza Ferro, Maria Inês Tiraboschi [UNESP] Ferro, Jesus Aparecido [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Universidade Federal de Ouro Preto (UFOP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Jacob, Tiago Rinaldi [UNESP] Laia, Marcelo Luiz de Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Carvalho, Flavia Maria de Souza Ferro, Maria Inês Tiraboschi [UNESP] Ferro, Jesus Aparecido [UNESP] |
description |
The type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Xanthomonas citri subsp. citri contains two copies of the T4SS, one in the chromosome and the other is plasmid-encoded. To understand the conditions that induce expression of the T4SS in Xcc, we analyzed, in vitro and in planta, the expression of 18 ORFs from the T4SS and 7 hypothetical flanking genes by RT-qPCR. As a positive control, we also evaluated the expression of 29 ORFs from the type III secretion system (T3SS), since these genes are known to be expressed during plant infection condition, but not necessarily in standard culture medium. From the 29 T3SS genes analyzed by qPCR, only hrpA was downregulated at 72 h after inoculation. All genes associated with the T4SS were downregulated on Citrus leaves 72 h after inoculation. Our results showed that unlike the T3SS, the T4SS is not induced during the infection process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-12-07T15:30:49Z 2015-12-07T15:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/763575 International Journal Of Microbiology, v. 2014, 2014. 1687-918X http://hdl.handle.net/11449/131011 10.1155/2014/763575 PMC3953393.pdf 0147241723612464 7587208482384059 24707292 PMC3953393 |
url |
http://dx.doi.org/10.1155/2014/763575 http://hdl.handle.net/11449/131011 |
identifier_str_mv |
International Journal Of Microbiology, v. 2014, 2014. 1687-918X 10.1155/2014/763575 PMC3953393.pdf 0147241723612464 7587208482384059 24707292 PMC3953393 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Journal Of Microbiology |
publisher.none.fl_str_mv |
International Journal Of Microbiology |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808129298420203520 |