Artificial methods of dentine caries induction: A hardness and morphological comparative study

Detalhes bibliográficos
Autor(a) principal: Marquezan, Marcela
Data de Publicação: 2009
Outros Autores: Correa, Fernanda Nahas P., Sanabe, Mariane Emi [UNESP], Rodrigues Filho, Leonardo Eloy, Hebling, Josimeri [UNESP], Guedes-Pinto, Antonio Carlos, Mendes, Fausto Medeiros
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2009.09.007
http://hdl.handle.net/11449/15780
Resumo: Objective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved.
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spelling Artificial methods of dentine caries induction: A hardness and morphological comparative studyArtificial cariesDentine cariesMicrohardnessScanning electron microscopypH-cyclingMicrobiologyGelObjective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Pro-Reitoria de Pesquisa da USP (Public resources)Univ São Paulo, Fac Odontol, Dept Orthodont & Pediat Dent, BR-05508 São Paulo, BrazilUniv São Paulo, Fac Odontol, Dept Dent Mat, BR-05508 São Paulo, BrazilUniv Estadual Paulista, Fac Odontol, Dept Orthodont & Pediat Dent, São Paulo, BrazilUniv Estadual Paulista, Fac Odontol, Dept Orthodont & Pediat Dent, São Paulo, BrazilCNPq: 484075/2006-3Pergamon-Elsevier B.V. LtdUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Marquezan, MarcelaCorrea, Fernanda Nahas P.Sanabe, Mariane Emi [UNESP]Rodrigues Filho, Leonardo EloyHebling, Josimeri [UNESP]Guedes-Pinto, Antonio CarlosMendes, Fausto Medeiros2013-09-30T18:31:29Z2014-05-20T13:44:57Z2013-09-30T18:31:29Z2014-05-20T13:44:57Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1111-1117http://dx.doi.org/10.1016/j.archoralbio.2009.09.007Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009.0003-9969http://hdl.handle.net/11449/1578010.1016/j.archoralbio.2009.09.007WOS:000273500800006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2024-09-26T14:22:32Zoai:repositorio.unesp.br:11449/15780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:22:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Artificial methods of dentine caries induction: A hardness and morphological comparative study
title Artificial methods of dentine caries induction: A hardness and morphological comparative study
spellingShingle Artificial methods of dentine caries induction: A hardness and morphological comparative study
Marquezan, Marcela
Artificial caries
Dentine caries
Microhardness
Scanning electron microscopy
pH-cycling
Microbiology
Gel
title_short Artificial methods of dentine caries induction: A hardness and morphological comparative study
title_full Artificial methods of dentine caries induction: A hardness and morphological comparative study
title_fullStr Artificial methods of dentine caries induction: A hardness and morphological comparative study
title_full_unstemmed Artificial methods of dentine caries induction: A hardness and morphological comparative study
title_sort Artificial methods of dentine caries induction: A hardness and morphological comparative study
author Marquezan, Marcela
author_facet Marquezan, Marcela
Correa, Fernanda Nahas P.
Sanabe, Mariane Emi [UNESP]
Rodrigues Filho, Leonardo Eloy
Hebling, Josimeri [UNESP]
Guedes-Pinto, Antonio Carlos
Mendes, Fausto Medeiros
author_role author
author2 Correa, Fernanda Nahas P.
Sanabe, Mariane Emi [UNESP]
Rodrigues Filho, Leonardo Eloy
Hebling, Josimeri [UNESP]
Guedes-Pinto, Antonio Carlos
Mendes, Fausto Medeiros
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Marquezan, Marcela
Correa, Fernanda Nahas P.
Sanabe, Mariane Emi [UNESP]
Rodrigues Filho, Leonardo Eloy
Hebling, Josimeri [UNESP]
Guedes-Pinto, Antonio Carlos
Mendes, Fausto Medeiros
dc.subject.por.fl_str_mv Artificial caries
Dentine caries
Microhardness
Scanning electron microscopy
pH-cycling
Microbiology
Gel
topic Artificial caries
Dentine caries
Microhardness
Scanning electron microscopy
pH-cycling
Microbiology
Gel
description Objective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2013-09-30T18:31:29Z
2013-09-30T18:31:29Z
2014-05-20T13:44:57Z
2014-05-20T13:44:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2009.09.007
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009.
0003-9969
http://hdl.handle.net/11449/15780
10.1016/j.archoralbio.2009.09.007
WOS:000273500800006
url http://dx.doi.org/10.1016/j.archoralbio.2009.09.007
http://hdl.handle.net/11449/15780
identifier_str_mv Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009.
0003-9969
10.1016/j.archoralbio.2009.09.007
WOS:000273500800006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
2.050
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1111-1117
dc.publisher.none.fl_str_mv Pergamon-Elsevier B.V. Ltd
publisher.none.fl_str_mv Pergamon-Elsevier B.V. Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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