Artificial methods of dentine caries induction: A hardness and morphological comparative study
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2009.09.007 http://hdl.handle.net/11449/15780 |
Resumo: | Objective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved. |
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Artificial methods of dentine caries induction: A hardness and morphological comparative studyArtificial cariesDentine cariesMicrohardnessScanning electron microscopypH-cyclingMicrobiologyGelObjective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Pro-Reitoria de Pesquisa da USP (Public resources)Univ São Paulo, Fac Odontol, Dept Orthodont & Pediat Dent, BR-05508 São Paulo, BrazilUniv São Paulo, Fac Odontol, Dept Dent Mat, BR-05508 São Paulo, BrazilUniv Estadual Paulista, Fac Odontol, Dept Orthodont & Pediat Dent, São Paulo, BrazilUniv Estadual Paulista, Fac Odontol, Dept Orthodont & Pediat Dent, São Paulo, BrazilCNPq: 484075/2006-3Pergamon-Elsevier B.V. LtdUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Marquezan, MarcelaCorrea, Fernanda Nahas P.Sanabe, Mariane Emi [UNESP]Rodrigues Filho, Leonardo EloyHebling, Josimeri [UNESP]Guedes-Pinto, Antonio CarlosMendes, Fausto Medeiros2013-09-30T18:31:29Z2014-05-20T13:44:57Z2013-09-30T18:31:29Z2014-05-20T13:44:57Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1111-1117http://dx.doi.org/10.1016/j.archoralbio.2009.09.007Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009.0003-9969http://hdl.handle.net/11449/1578010.1016/j.archoralbio.2009.09.007WOS:000273500800006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2024-09-26T14:22:32Zoai:repositorio.unesp.br:11449/15780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:22:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
title |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
spellingShingle |
Artificial methods of dentine caries induction: A hardness and morphological comparative study Marquezan, Marcela Artificial caries Dentine caries Microhardness Scanning electron microscopy pH-cycling Microbiology Gel |
title_short |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
title_full |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
title_fullStr |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
title_full_unstemmed |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
title_sort |
Artificial methods of dentine caries induction: A hardness and morphological comparative study |
author |
Marquezan, Marcela |
author_facet |
Marquezan, Marcela Correa, Fernanda Nahas P. Sanabe, Mariane Emi [UNESP] Rodrigues Filho, Leonardo Eloy Hebling, Josimeri [UNESP] Guedes-Pinto, Antonio Carlos Mendes, Fausto Medeiros |
author_role |
author |
author2 |
Correa, Fernanda Nahas P. Sanabe, Mariane Emi [UNESP] Rodrigues Filho, Leonardo Eloy Hebling, Josimeri [UNESP] Guedes-Pinto, Antonio Carlos Mendes, Fausto Medeiros |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Marquezan, Marcela Correa, Fernanda Nahas P. Sanabe, Mariane Emi [UNESP] Rodrigues Filho, Leonardo Eloy Hebling, Josimeri [UNESP] Guedes-Pinto, Antonio Carlos Mendes, Fausto Medeiros |
dc.subject.por.fl_str_mv |
Artificial caries Dentine caries Microhardness Scanning electron microscopy pH-cycling Microbiology Gel |
topic |
Artificial caries Dentine caries Microhardness Scanning electron microscopy pH-cycling Microbiology Gel |
description |
Objective: To assess the ability of two chemical and a microbiological methods to produce dentine caries lesions resembling naturally developed dentine caries lesions.Design: Forty sound second primary molars were divided into four experimental groups according to the method to produce artificial caries lesions: (1) sound (negative control); (2) acidified gel; (3) pH-cycling; and (4) microbiological, all for 14 days. Ten second primary molars presenting natural dentine caries lesions comprised the (5) positive control group. After the artificial caries induction, all samples were longitudinally sectioned and polished in order to obtain Knoop microhardness values from 10 to 500 mu m depth from the bottom of the cavities. Morphological analysis of the surfaces was carried out by SEM. Hardness data were compared among the five experimental groups using One-Way ANOVA and post hoc SNK's test.Results: The hardness values of chemically created caries-like lesions did not differ from that of natural caries lesions on shallower depths. The results indicated that chemical caries induction methods promote a superficial demineralization and that pH-cycling is more effective than acidified gel. The former, produced a thicker layer of demineralization, with similar hardness values than natural lesions. Despite the microbiological method provided an excessive softness of the primary dentine, this method presented morphology more comparable to natural lesions.Conclusions: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal. (C) 2009 Elsevier Ltd. All rights reserved. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 2013-09-30T18:31:29Z 2013-09-30T18:31:29Z 2014-05-20T13:44:57Z 2014-05-20T13:44:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2009.09.007 Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009. 0003-9969 http://hdl.handle.net/11449/15780 10.1016/j.archoralbio.2009.09.007 WOS:000273500800006 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2009.09.007 http://hdl.handle.net/11449/15780 |
identifier_str_mv |
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 12, p. 1111-1117, 2009. 0003-9969 10.1016/j.archoralbio.2009.09.007 WOS:000273500800006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 2.050 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1111-1117 |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546483146817536 |