Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Vinícius Peixoto [UNESP]
Data de Publicação: 2017
Outros Autores: Da Rocha, Cláudia Quintino, Périco, Larissa Lucena [UNESP], De Cássia dos Santos, Raquel, Ohara, Rie [UNESP], Nishijima, Catarine Massucato [UNESP], Queiroz, Emerson Ferreira, Wolfender, Jean-Luc, Da Rocha, Lúcia Regina Machado [UNESP], Santos, Adair Roberto Soares, Vilegas, Wagner [UNESP], Hiruma-Lima, Clélia Akiko [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ijms18112304
http://hdl.handle.net/11449/179343
Resumo: Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.
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spelling Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureauAntinociceptive effectArrabidaea brachypoda (dc) bureauBignoniaceaePainArrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Biosciences Institute Department of PhysiologyDepartment of Chemistry Federal University of Maranhão, Av. dos Portugueses, 1966â-BacangaClinical Pharmacology and Gastroenterology Unit (USF)School of Pharmaceutical Sciences EPGL University of Geneva University of Lausanne, CMU-Rue Michel-Servet 1Federal University of Santa Catarina Laboratory of Neurobiology of Pain and Inflammation Department of Physiological Sciences Center of Biological Sciences, TrindadeBiosciences Institute São Paulo State University (UNESP)São Paulo State University (UNESP) Biosciences Institute Department of PhysiologyBiosciences Institute São Paulo State University (UNESP)FAPESP: 2009/52237-9CNPq: 305570/2012-9Universidade Estadual Paulista (Unesp)Federal University of MaranhãoClinical Pharmacology and Gastroenterology Unit (USF)University of LausanneUniversidade Federal de Santa Catarina (UFSC)Rodrigues, Vinícius Peixoto [UNESP]Da Rocha, Cláudia QuintinoPérico, Larissa Lucena [UNESP]De Cássia dos Santos, RaquelOhara, Rie [UNESP]Nishijima, Catarine Massucato [UNESP]Queiroz, Emerson FerreiraWolfender, Jean-LucDa Rocha, Lúcia Regina Machado [UNESP]Santos, Adair Roberto SoaresVilegas, Wagner [UNESP]Hiruma-Lima, Clélia Akiko [UNESP]2018-12-11T17:34:47Z2018-12-11T17:34:47Z2017-11-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/ijms18112304International Journal of Molecular Sciences, v. 18, n. 11, 2017.1422-00671661-6596http://hdl.handle.net/11449/17934310.3390/ijms181123042-s2.0-850335541182-s2.0-85033554118.pdf38145049013868440000-0002-8645-3777Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciences1,260info:eu-repo/semantics/openAccess2023-10-25T06:06:51Zoai:repositorio.unesp.br:11449/179343Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:53:00.280874Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
title Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
spellingShingle Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
Rodrigues, Vinícius Peixoto [UNESP]
Antinociceptive effect
Arrabidaea brachypoda (dc) bureau
Bignoniaceae
Pain
title_short Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
title_full Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
title_fullStr Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
title_full_unstemmed Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
title_sort Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
author Rodrigues, Vinícius Peixoto [UNESP]
author_facet Rodrigues, Vinícius Peixoto [UNESP]
Da Rocha, Cláudia Quintino
Périco, Larissa Lucena [UNESP]
De Cássia dos Santos, Raquel
Ohara, Rie [UNESP]
Nishijima, Catarine Massucato [UNESP]
Queiroz, Emerson Ferreira
Wolfender, Jean-Luc
Da Rocha, Lúcia Regina Machado [UNESP]
Santos, Adair Roberto Soares
Vilegas, Wagner [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
author_role author
author2 Da Rocha, Cláudia Quintino
Périco, Larissa Lucena [UNESP]
De Cássia dos Santos, Raquel
Ohara, Rie [UNESP]
Nishijima, Catarine Massucato [UNESP]
Queiroz, Emerson Ferreira
Wolfender, Jean-Luc
Da Rocha, Lúcia Regina Machado [UNESP]
Santos, Adair Roberto Soares
Vilegas, Wagner [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Federal University of Maranhão
Clinical Pharmacology and Gastroenterology Unit (USF)
University of Lausanne
Universidade Federal de Santa Catarina (UFSC)
dc.contributor.author.fl_str_mv Rodrigues, Vinícius Peixoto [UNESP]
Da Rocha, Cláudia Quintino
Périco, Larissa Lucena [UNESP]
De Cássia dos Santos, Raquel
Ohara, Rie [UNESP]
Nishijima, Catarine Massucato [UNESP]
Queiroz, Emerson Ferreira
Wolfender, Jean-Luc
Da Rocha, Lúcia Regina Machado [UNESP]
Santos, Adair Roberto Soares
Vilegas, Wagner [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
dc.subject.por.fl_str_mv Antinociceptive effect
Arrabidaea brachypoda (dc) bureau
Bignoniaceae
Pain
topic Antinociceptive effect
Arrabidaea brachypoda (dc) bureau
Bignoniaceae
Pain
description Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-02
2018-12-11T17:34:47Z
2018-12-11T17:34:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ijms18112304
International Journal of Molecular Sciences, v. 18, n. 11, 2017.
1422-0067
1661-6596
http://hdl.handle.net/11449/179343
10.3390/ijms18112304
2-s2.0-85033554118
2-s2.0-85033554118.pdf
3814504901386844
0000-0002-8645-3777
url http://dx.doi.org/10.3390/ijms18112304
http://hdl.handle.net/11449/179343
identifier_str_mv International Journal of Molecular Sciences, v. 18, n. 11, 2017.
1422-0067
1661-6596
10.3390/ijms18112304
2-s2.0-85033554118
2-s2.0-85033554118.pdf
3814504901386844
0000-0002-8645-3777
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Molecular Sciences
1,260
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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