Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms18112304 http://hdl.handle.net/11449/179343 |
Resumo: | Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief. |
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Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureauAntinociceptive effectArrabidaea brachypoda (dc) bureauBignoniaceaePainArrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Biosciences Institute Department of PhysiologyDepartment of Chemistry Federal University of Maranhão, Av. dos Portugueses, 1966â-BacangaClinical Pharmacology and Gastroenterology Unit (USF)School of Pharmaceutical Sciences EPGL University of Geneva University of Lausanne, CMU-Rue Michel-Servet 1Federal University of Santa Catarina Laboratory of Neurobiology of Pain and Inflammation Department of Physiological Sciences Center of Biological Sciences, TrindadeBiosciences Institute São Paulo State University (UNESP)São Paulo State University (UNESP) Biosciences Institute Department of PhysiologyBiosciences Institute São Paulo State University (UNESP)FAPESP: 2009/52237-9CNPq: 305570/2012-9Universidade Estadual Paulista (Unesp)Federal University of MaranhãoClinical Pharmacology and Gastroenterology Unit (USF)University of LausanneUniversidade Federal de Santa Catarina (UFSC)Rodrigues, Vinícius Peixoto [UNESP]Da Rocha, Cláudia QuintinoPérico, Larissa Lucena [UNESP]De Cássia dos Santos, RaquelOhara, Rie [UNESP]Nishijima, Catarine Massucato [UNESP]Queiroz, Emerson FerreiraWolfender, Jean-LucDa Rocha, Lúcia Regina Machado [UNESP]Santos, Adair Roberto SoaresVilegas, Wagner [UNESP]Hiruma-Lima, Clélia Akiko [UNESP]2018-12-11T17:34:47Z2018-12-11T17:34:47Z2017-11-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/ijms18112304International Journal of Molecular Sciences, v. 18, n. 11, 2017.1422-00671661-6596http://hdl.handle.net/11449/17934310.3390/ijms181123042-s2.0-850335541182-s2.0-85033554118.pdf38145049013868440000-0002-8645-3777Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciences1,260info:eu-repo/semantics/openAccess2023-10-25T06:06:51Zoai:repositorio.unesp.br:11449/179343Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:53:00.280874Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
title |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
spellingShingle |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau Rodrigues, Vinícius Peixoto [UNESP] Antinociceptive effect Arrabidaea brachypoda (dc) bureau Bignoniaceae Pain |
title_short |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
title_full |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
title_fullStr |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
title_full_unstemmed |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
title_sort |
Involvement of opioid system, TRPM8, and ASIC receptors in antinociceptive effect of Arrabidaea brachypoda (DC) bureau |
author |
Rodrigues, Vinícius Peixoto [UNESP] |
author_facet |
Rodrigues, Vinícius Peixoto [UNESP] Da Rocha, Cláudia Quintino Périco, Larissa Lucena [UNESP] De Cássia dos Santos, Raquel Ohara, Rie [UNESP] Nishijima, Catarine Massucato [UNESP] Queiroz, Emerson Ferreira Wolfender, Jean-Luc Da Rocha, Lúcia Regina Machado [UNESP] Santos, Adair Roberto Soares Vilegas, Wagner [UNESP] Hiruma-Lima, Clélia Akiko [UNESP] |
author_role |
author |
author2 |
Da Rocha, Cláudia Quintino Périco, Larissa Lucena [UNESP] De Cássia dos Santos, Raquel Ohara, Rie [UNESP] Nishijima, Catarine Massucato [UNESP] Queiroz, Emerson Ferreira Wolfender, Jean-Luc Da Rocha, Lúcia Regina Machado [UNESP] Santos, Adair Roberto Soares Vilegas, Wagner [UNESP] Hiruma-Lima, Clélia Akiko [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Maranhão Clinical Pharmacology and Gastroenterology Unit (USF) University of Lausanne Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
Rodrigues, Vinícius Peixoto [UNESP] Da Rocha, Cláudia Quintino Périco, Larissa Lucena [UNESP] De Cássia dos Santos, Raquel Ohara, Rie [UNESP] Nishijima, Catarine Massucato [UNESP] Queiroz, Emerson Ferreira Wolfender, Jean-Luc Da Rocha, Lúcia Regina Machado [UNESP] Santos, Adair Roberto Soares Vilegas, Wagner [UNESP] Hiruma-Lima, Clélia Akiko [UNESP] |
dc.subject.por.fl_str_mv |
Antinociceptive effect Arrabidaea brachypoda (dc) bureau Bignoniaceae Pain |
topic |
Antinociceptive effect Arrabidaea brachypoda (dc) bureau Bignoniaceae Pain |
description |
Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “ció-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-02 2018-12-11T17:34:47Z 2018-12-11T17:34:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms18112304 International Journal of Molecular Sciences, v. 18, n. 11, 2017. 1422-0067 1661-6596 http://hdl.handle.net/11449/179343 10.3390/ijms18112304 2-s2.0-85033554118 2-s2.0-85033554118.pdf 3814504901386844 0000-0002-8645-3777 |
url |
http://dx.doi.org/10.3390/ijms18112304 http://hdl.handle.net/11449/179343 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 18, n. 11, 2017. 1422-0067 1661-6596 10.3390/ijms18112304 2-s2.0-85033554118 2-s2.0-85033554118.pdf 3814504901386844 0000-0002-8645-3777 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences 1,260 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128578258206720 |