Direct Analysis of Doping Agents in Raw Urine Using Hydrophobic Paper Spray Mass Spectrometry

• Autor(a) principal: Rossini, Eduardo Luiz [UNESP]
• Data de Publicação: 2020
• Outros Autores: Kulyk, Dmytro S., Ansu-Gyeabourh, Emelia, Sahraeian, Taghi, Pezza, Helena Redigolo [UNESP], Badu-Tawiah, Abraham K.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1021/jasms.0c00063; http://hdl.handle.net/11449/200553
• topic: anabolic agent; analytical methods; diuretic; doping; paper modification; paper spray ionization

In this study, the direct analysis of doping agents in urine samples with no sample preparation by a modified paper spray mass spectrometry (PS-MS) methodology has been demonstrated for the first time. We have described a paper surface treatment with trichloromethylsilane using a gas-phase reaction to increase the ionization of target compounds. This approach was applied for the analysis of two classes of banned substances in urine samples: anabolic agents (trenbolone and clenbuterol) and diuretics (furosemide and hydrochlorothiazide). Under optimized conditions, the developed methods presented satisfactory repeatability, and an analysis of variance showed linearity without lack-of-fit. Highly sensitive detections as low as sub-nanogram per milliliter levels, which is below the minimum required performance levels proposed by the World Anti-Doping Agency, have been reached using the hydrophobic PS-MS analysis without any preconcentration and cleanup step.