Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study

Detalhes bibliográficos
Autor(a) principal: Nai, Gisele Alborghetti
Data de Publicação: 2021
Outros Autores: Medina, Denis Aloísio Lopes, Martelli, Cesar Alberto Talavera, de Oliveira, Mayla Silva Cayres, Portelinha, Maria Júlia Schadeck, Henriques, Bruno Carvalho, Caldeira, Isadora Delfino, Almeida, Mércia de Carvalho, Eller, Lizziane Kretli Winkelstroter, Neto, Fausto Viterbo de Oliveira, Marques, Mariângela Esther Alencar [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1097/MD.0000000000025285
http://hdl.handle.net/11449/207569
Resumo: ABSTRACT: Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination.The following microorganisms were evaluated: Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Enterococcus faecalis. Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool.The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses.Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis, in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.
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spelling Does washing medical devices before and after use decrease bacterial contamination?: An in vitro studyABSTRACT: Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination.The following microorganisms were evaluated: Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Enterococcus faecalis. Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool.The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses.Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis, in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.Department of PathologyGraduate Program in Health SciencesGraduate Program in Animal ScienceSchool of MedicineDepartment of SurgeryLaboratory of Clinical AnalysisDepartment of Microbiology Universidade do Oeste Paulista (UNOESTE)Department of Plastic SurgeryDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP)Department of Pathology Botucatu Medical School São Paulo State University (UNESP)Graduate Program in Health SciencesGraduate Program in Animal ScienceSchool of MedicineLaboratory of Clinical AnalysisUniversidade do Oeste Paulista (UNOESTE)Universidade Estadual Paulista (Unesp)Nai, Gisele AlborghettiMedina, Denis Aloísio LopesMartelli, Cesar Alberto Talaverade Oliveira, Mayla Silva CayresPortelinha, Maria Júlia SchadeckHenriques, Bruno CarvalhoCaldeira, Isadora DelfinoAlmeida, Mércia de CarvalhoEller, Lizziane Kretli WinkelstroterNeto, Fausto Viterbo de OliveiraMarques, Mariângela Esther Alencar [UNESP]2021-06-25T10:57:26Z2021-06-25T10:57:26Z2021-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee25285http://dx.doi.org/10.1097/MD.0000000000025285Medicine, v. 100, n. 13, p. e25285-, 2021.1536-5964http://hdl.handle.net/11449/20756910.1097/MD.00000000000252852-s2.0-85103745935Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedicineinfo:eu-repo/semantics/openAccess2024-09-03T13:18:15Zoai:repositorio.unesp.br:11449/207569Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
title Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
spellingShingle Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
Nai, Gisele Alborghetti
title_short Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
title_full Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
title_fullStr Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
title_full_unstemmed Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
title_sort Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study
author Nai, Gisele Alborghetti
author_facet Nai, Gisele Alborghetti
Medina, Denis Aloísio Lopes
Martelli, Cesar Alberto Talavera
de Oliveira, Mayla Silva Cayres
Portelinha, Maria Júlia Schadeck
Henriques, Bruno Carvalho
Caldeira, Isadora Delfino
Almeida, Mércia de Carvalho
Eller, Lizziane Kretli Winkelstroter
Neto, Fausto Viterbo de Oliveira
Marques, Mariângela Esther Alencar [UNESP]
author_role author
author2 Medina, Denis Aloísio Lopes
Martelli, Cesar Alberto Talavera
de Oliveira, Mayla Silva Cayres
Portelinha, Maria Júlia Schadeck
Henriques, Bruno Carvalho
Caldeira, Isadora Delfino
Almeida, Mércia de Carvalho
Eller, Lizziane Kretli Winkelstroter
Neto, Fausto Viterbo de Oliveira
Marques, Mariângela Esther Alencar [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Graduate Program in Health Sciences
Graduate Program in Animal Science
School of Medicine
Laboratory of Clinical Analysis
Universidade do Oeste Paulista (UNOESTE)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nai, Gisele Alborghetti
Medina, Denis Aloísio Lopes
Martelli, Cesar Alberto Talavera
de Oliveira, Mayla Silva Cayres
Portelinha, Maria Júlia Schadeck
Henriques, Bruno Carvalho
Caldeira, Isadora Delfino
Almeida, Mércia de Carvalho
Eller, Lizziane Kretli Winkelstroter
Neto, Fausto Viterbo de Oliveira
Marques, Mariângela Esther Alencar [UNESP]
description ABSTRACT: Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination.The following microorganisms were evaluated: Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Enterococcus faecalis. Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool.The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses.Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis, in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:57:26Z
2021-06-25T10:57:26Z
2021-04-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1097/MD.0000000000025285
Medicine, v. 100, n. 13, p. e25285-, 2021.
1536-5964
http://hdl.handle.net/11449/207569
10.1097/MD.0000000000025285
2-s2.0-85103745935
url http://dx.doi.org/10.1097/MD.0000000000025285
http://hdl.handle.net/11449/207569
identifier_str_mv Medicine, v. 100, n. 13, p. e25285-, 2021.
1536-5964
10.1097/MD.0000000000025285
2-s2.0-85103745935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv e25285
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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