ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2217/pgs-2022-0079 http://hdl.handle.net/11449/241589 |
Resumo: | Aim: This work examined whether ARG1 (rs2781659, rs2781667, rs2246012 and rs17599586) and ARG2 (rs3742879 and rs10483801) single-nucleotide polymorphisms (SNPs) are associated with antihypertensive therapy responsiveness in preeclampsia (PE) and their effects on arginase isoforms and nitrite concentrations in responsive and nonresponsive patients. Methods: SNP genotypes were determined by TaqMan assays. Plasma arginase levels were measured by ELISA and nitrite concentrations were measured using an ozone-based chemiluminescence assay. Results: The G allele for ARG2 rs3742879 (A>G) was less frequent in nonresponsive compared with responsive patients (15.5% vs 24.7%, respectively) and the G carriers of the nonresponsive subgroup had lower arginase 2 (9.2 ± 7.5 ng/ml vs 19.1 ± 17.3 ng/ml) and higher nitrite concentrations (110.2 ± 52.8 nM vs 78.5 ± 37.9 nM) than carriers of the AA genotype (all p < 0.05). Conclusion: ARG2 SNP rs3742879 is associated with diminished arginase 2 levels and increased nitric oxide formation in nonresponsive PE patients. |
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ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsiaARG2arginase 2genetic polymorphismshypertensionnitric oxidenitritepharmacogeneticspreeclampsiaAim: This work examined whether ARG1 (rs2781659, rs2781667, rs2246012 and rs17599586) and ARG2 (rs3742879 and rs10483801) single-nucleotide polymorphisms (SNPs) are associated with antihypertensive therapy responsiveness in preeclampsia (PE) and their effects on arginase isoforms and nitrite concentrations in responsive and nonresponsive patients. Methods: SNP genotypes were determined by TaqMan assays. Plasma arginase levels were measured by ELISA and nitrite concentrations were measured using an ozone-based chemiluminescence assay. Results: The G allele for ARG2 rs3742879 (A>G) was less frequent in nonresponsive compared with responsive patients (15.5% vs 24.7%, respectively) and the G carriers of the nonresponsive subgroup had lower arginase 2 (9.2 ± 7.5 ng/ml vs 19.1 ± 17.3 ng/ml) and higher nitrite concentrations (110.2 ± 52.8 nM vs 78.5 ± 37.9 nM) than carriers of the AA genotype (all p < 0.05). Conclusion: ARG2 SNP rs3742879 is associated with diminished arginase 2 levels and increased nitric oxide formation in nonresponsive PE patients.Department of Genetics Ecology and Evolution Institute of Biological Sciences Federal University of Minas Gerais (UFMG), MGDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, BotucatuDepartment of Clinical Analyses Toxicology and Food Science School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo (USP), Ribeirao PretoDepartment of Psychiatric Nursing and Human Sciences Ribeirao Preto School of Nursing University of Sao Paulo (USP), Ribeirao PretoDepartment of Gynecology and Obstetrics University of Sao Paulo (USP), Ribeirao PretoDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, BotucatuUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Luizon, Marcelo R.Pinto-Souza, Caroline C. [UNESP]Coeli-Lacchini, FernandaLacchini, RiccardoCavalli, Ricardo C.Sandrim, Valeria C. [UNESP]2023-03-01T21:11:49Z2023-03-01T21:11:49Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article713-722http://dx.doi.org/10.2217/pgs-2022-0079Pharmacogenomics, v. 23, n. 13, p. 713-722, 2022.1744-80421462-2416http://hdl.handle.net/11449/24158910.2217/pgs-2022-00792-s2.0-85137134448Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmacogenomicsinfo:eu-repo/semantics/openAccess2023-03-01T21:11:49Zoai:repositorio.unesp.br:11449/241589Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:54.221492Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
title |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
spellingShingle |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia Luizon, Marcelo R. ARG2 arginase 2 genetic polymorphisms hypertension nitric oxide nitrite pharmacogenetics preeclampsia |
title_short |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
title_full |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
title_fullStr |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
title_full_unstemmed |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
title_sort |
ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia |
author |
Luizon, Marcelo R. |
author_facet |
Luizon, Marcelo R. Pinto-Souza, Caroline C. [UNESP] Coeli-Lacchini, Fernanda Lacchini, Riccardo Cavalli, Ricardo C. Sandrim, Valeria C. [UNESP] |
author_role |
author |
author2 |
Pinto-Souza, Caroline C. [UNESP] Coeli-Lacchini, Fernanda Lacchini, Riccardo Cavalli, Ricardo C. Sandrim, Valeria C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Luizon, Marcelo R. Pinto-Souza, Caroline C. [UNESP] Coeli-Lacchini, Fernanda Lacchini, Riccardo Cavalli, Ricardo C. Sandrim, Valeria C. [UNESP] |
dc.subject.por.fl_str_mv |
ARG2 arginase 2 genetic polymorphisms hypertension nitric oxide nitrite pharmacogenetics preeclampsia |
topic |
ARG2 arginase 2 genetic polymorphisms hypertension nitric oxide nitrite pharmacogenetics preeclampsia |
description |
Aim: This work examined whether ARG1 (rs2781659, rs2781667, rs2246012 and rs17599586) and ARG2 (rs3742879 and rs10483801) single-nucleotide polymorphisms (SNPs) are associated with antihypertensive therapy responsiveness in preeclampsia (PE) and their effects on arginase isoforms and nitrite concentrations in responsive and nonresponsive patients. Methods: SNP genotypes were determined by TaqMan assays. Plasma arginase levels were measured by ELISA and nitrite concentrations were measured using an ozone-based chemiluminescence assay. Results: The G allele for ARG2 rs3742879 (A>G) was less frequent in nonresponsive compared with responsive patients (15.5% vs 24.7%, respectively) and the G carriers of the nonresponsive subgroup had lower arginase 2 (9.2 ± 7.5 ng/ml vs 19.1 ± 17.3 ng/ml) and higher nitrite concentrations (110.2 ± 52.8 nM vs 78.5 ± 37.9 nM) than carriers of the AA genotype (all p < 0.05). Conclusion: ARG2 SNP rs3742879 is associated with diminished arginase 2 levels and increased nitric oxide formation in nonresponsive PE patients. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T21:11:49Z 2023-03-01T21:11:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2217/pgs-2022-0079 Pharmacogenomics, v. 23, n. 13, p. 713-722, 2022. 1744-8042 1462-2416 http://hdl.handle.net/11449/241589 10.2217/pgs-2022-0079 2-s2.0-85137134448 |
url |
http://dx.doi.org/10.2217/pgs-2022-0079 http://hdl.handle.net/11449/241589 |
identifier_str_mv |
Pharmacogenomics, v. 23, n. 13, p. 713-722, 2022. 1744-8042 1462-2416 10.2217/pgs-2022-0079 2-s2.0-85137134448 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmacogenomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
713-722 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128320195264512 |