The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1203/00006450-198805000-00008 http://hdl.handle.net/11449/219124 |
Resumo: | There is a paucity of information on the significance of insulin on neonatal cerebral glucose metabolism. The effect of insulin on neonatal cerebral glucose uptake and cerebral cortical metabolic intermediates was investigated with the euglycemic hyperinsulinemic clamp in unanesthetized beagles during the first day of life. Insulin was infused at various rates to sustain an elevated steady state plasma insulin concentration in individual pups. Furthermore, blood glucose and 2-deoxyglucose levels were also maintained (clamped) in a steady state by infusion of glucose and 2-deoxy-[14C]-gIucose. Mean (± SD) plasma insulin levels were 20 ± 12 and 2971 ± 3386 (33-14330) nU/ml in control and hyperinsulinemic pups. Blood glucose concentration was 4.43 ± 2.64 mM during basal periods and 4.54 ± 2.87 mM during the clamp period in study pups. Basal fasting glucose utilization in study pups was 43.9 ± 24 μmol/kg/min and increased to 60.9 ± 35.2 ^mol/ kg/min (p < 0.001) during hyperinsulinemia. Immediately after the euglycemic hyperinsulinemic clamp or fasting in control pups, the cerebral cortex was frozen to the temperature of liquid nitrogen. No differences were noted for any cerebral cortical intermediate between the two pup groups. In addition, there was no relationship between the cerebral intermediates concentration when analyzed as a function of plasma insulin levels. The uptake of cerebral 2-deoxyglucose was analyzed as a function of plasma insulin concentration (120-6900 μU/ml). Brain tissue demonstrated a positive linear relationship for 2-deoxyglucose uptake as a function of plasma insulin concentration. Although static determination of brain metabolites were not influenced by hyperinsulinemia, there was a positive effect of insulin on cerebral glucose uptake. Either directly or indirectly, insulin may increase brain glucose utilization in the newborn dog. © 1988 International Pediatric Research Foundation, Inc. |
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The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beaglesThere is a paucity of information on the significance of insulin on neonatal cerebral glucose metabolism. The effect of insulin on neonatal cerebral glucose uptake and cerebral cortical metabolic intermediates was investigated with the euglycemic hyperinsulinemic clamp in unanesthetized beagles during the first day of life. Insulin was infused at various rates to sustain an elevated steady state plasma insulin concentration in individual pups. Furthermore, blood glucose and 2-deoxyglucose levels were also maintained (clamped) in a steady state by infusion of glucose and 2-deoxy-[14C]-gIucose. Mean (± SD) plasma insulin levels were 20 ± 12 and 2971 ± 3386 (33-14330) nU/ml in control and hyperinsulinemic pups. Blood glucose concentration was 4.43 ± 2.64 mM during basal periods and 4.54 ± 2.87 mM during the clamp period in study pups. Basal fasting glucose utilization in study pups was 43.9 ± 24 μmol/kg/min and increased to 60.9 ± 35.2 ^mol/ kg/min (p < 0.001) during hyperinsulinemia. Immediately after the euglycemic hyperinsulinemic clamp or fasting in control pups, the cerebral cortex was frozen to the temperature of liquid nitrogen. No differences were noted for any cerebral cortical intermediate between the two pup groups. In addition, there was no relationship between the cerebral intermediates concentration when analyzed as a function of plasma insulin levels. The uptake of cerebral 2-deoxyglucose was analyzed as a function of plasma insulin concentration (120-6900 μU/ml). Brain tissue demonstrated a positive linear relationship for 2-deoxyglucose uptake as a function of plasma insulin concentration. Although static determination of brain metabolites were not influenced by hyperinsulinemia, there was a positive effect of insulin on cerebral glucose uptake. Either directly or indirectly, insulin may increase brain glucose utilization in the newborn dog. © 1988 International Pediatric Research Foundation, Inc.Department of Pediatrics Case Western Reserve University Rainbow Babies Childrens Hospital, Cleveland, OH, 44106Department of Pediatrics UNESP, Sao PauloDepartment of Pediatrics UNESP, Sao PauloChildrens HospitalUniversidade Estadual Paulista (UNESP)Trindade, Cleide [UNESP]Huang, Marian [UNESP]Hulman, Sonia [UNESP]Reef, Susan [UNESP]Kliegman, Robert [UNESP]2022-04-28T18:53:55Z2022-04-28T18:53:55Z1988-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article474-479http://dx.doi.org/10.1203/00006450-198805000-00008Pediatric Research, v. 23, n. 5, p. 474-479, 1988.1530-04470031-3998http://hdl.handle.net/11449/21912410.1203/00006450-198805000-000082-s2.0-0023912640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPediatric Researchinfo:eu-repo/semantics/openAccess2022-04-28T18:53:55Zoai:repositorio.unesp.br:11449/219124Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:51:15.435158Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
title |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
spellingShingle |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles Trindade, Cleide [UNESP] |
title_short |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
title_full |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
title_fullStr |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
title_full_unstemmed |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
title_sort |
The effect of euglycemic hyperinsulinemia on cerebral cortical glucose metabolism in newborn beagles |
author |
Trindade, Cleide [UNESP] |
author_facet |
Trindade, Cleide [UNESP] Huang, Marian [UNESP] Hulman, Sonia [UNESP] Reef, Susan [UNESP] Kliegman, Robert [UNESP] |
author_role |
author |
author2 |
Huang, Marian [UNESP] Hulman, Sonia [UNESP] Reef, Susan [UNESP] Kliegman, Robert [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Childrens Hospital Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Trindade, Cleide [UNESP] Huang, Marian [UNESP] Hulman, Sonia [UNESP] Reef, Susan [UNESP] Kliegman, Robert [UNESP] |
description |
There is a paucity of information on the significance of insulin on neonatal cerebral glucose metabolism. The effect of insulin on neonatal cerebral glucose uptake and cerebral cortical metabolic intermediates was investigated with the euglycemic hyperinsulinemic clamp in unanesthetized beagles during the first day of life. Insulin was infused at various rates to sustain an elevated steady state plasma insulin concentration in individual pups. Furthermore, blood glucose and 2-deoxyglucose levels were also maintained (clamped) in a steady state by infusion of glucose and 2-deoxy-[14C]-gIucose. Mean (± SD) plasma insulin levels were 20 ± 12 and 2971 ± 3386 (33-14330) nU/ml in control and hyperinsulinemic pups. Blood glucose concentration was 4.43 ± 2.64 mM during basal periods and 4.54 ± 2.87 mM during the clamp period in study pups. Basal fasting glucose utilization in study pups was 43.9 ± 24 μmol/kg/min and increased to 60.9 ± 35.2 ^mol/ kg/min (p < 0.001) during hyperinsulinemia. Immediately after the euglycemic hyperinsulinemic clamp or fasting in control pups, the cerebral cortex was frozen to the temperature of liquid nitrogen. No differences were noted for any cerebral cortical intermediate between the two pup groups. In addition, there was no relationship between the cerebral intermediates concentration when analyzed as a function of plasma insulin levels. The uptake of cerebral 2-deoxyglucose was analyzed as a function of plasma insulin concentration (120-6900 μU/ml). Brain tissue demonstrated a positive linear relationship for 2-deoxyglucose uptake as a function of plasma insulin concentration. Although static determination of brain metabolites were not influenced by hyperinsulinemia, there was a positive effect of insulin on cerebral glucose uptake. Either directly or indirectly, insulin may increase brain glucose utilization in the newborn dog. © 1988 International Pediatric Research Foundation, Inc. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-01-01 2022-04-28T18:53:55Z 2022-04-28T18:53:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1203/00006450-198805000-00008 Pediatric Research, v. 23, n. 5, p. 474-479, 1988. 1530-0447 0031-3998 http://hdl.handle.net/11449/219124 10.1203/00006450-198805000-00008 2-s2.0-0023912640 |
url |
http://dx.doi.org/10.1203/00006450-198805000-00008 http://hdl.handle.net/11449/219124 |
identifier_str_mv |
Pediatric Research, v. 23, n. 5, p. 474-479, 1988. 1530-0447 0031-3998 10.1203/00006450-198805000-00008 2-s2.0-0023912640 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pediatric Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
474-479 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129366627975168 |