Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bcab.2019.101268 http://hdl.handle.net/11449/197533 |
Resumo: | Burkholderia glumae MA13 was isolated from soil samples of Atlantic rain forest ecosystem as a new bacterial strain for polyhydroxyalkanoate (PHA) production from crude glycerol as sole carbon source. Among 107 glycerol consuming bacterial isolates, B. glumae MA13 was cultivated in shake flask experiments in order to verify its PHA production capability from crude glycerol besides waste cooking oil and sugarcane molasses free of pretreatment, showing intracellular poly(3-hydroxybutyrate) [P(3HB)] accumulation values of 51.3, 51.4 and 49% of cell dry weight (CDW), respectively. The addition of propionic or hexanoic acids to the media containing crude glycerol resulted in the copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] comprised of up to 32.1 mol% of 3HV monomer or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a maximum of 0.3 mol% of 3HHx, respectively. Fed-batch cultivation performed in a 10 L-scale bioreactor showed a maximum P(3HB-co-3HV) production of 9.9 g/L, which was 65.1% of total biomass and consisted of 21.8 mol% of 3HV monomer, with a maximum volumetric productivity of 0.22 g/(L h). The 3HV conversion yield achieved 1.04 g/g after 48 h cultivation, which was 76.7% of the maximum theoretical yield. B. glumae MA13 has showed to be an adapted bacterial strain to the synthesis of bioplastics from biofuel byproducts, and so from here it has been revealed as a promising PHA producer for an associated production set which has been considered as a prominent and ecologically friendly alternative to petrochemical plastics and fuels. |
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Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerolBiodieselBioplasticBurkholderia glumaeGlycerolPolyhydroxyalkanoatesBurkholderia glumae MA13 was isolated from soil samples of Atlantic rain forest ecosystem as a new bacterial strain for polyhydroxyalkanoate (PHA) production from crude glycerol as sole carbon source. Among 107 glycerol consuming bacterial isolates, B. glumae MA13 was cultivated in shake flask experiments in order to verify its PHA production capability from crude glycerol besides waste cooking oil and sugarcane molasses free of pretreatment, showing intracellular poly(3-hydroxybutyrate) [P(3HB)] accumulation values of 51.3, 51.4 and 49% of cell dry weight (CDW), respectively. The addition of propionic or hexanoic acids to the media containing crude glycerol resulted in the copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] comprised of up to 32.1 mol% of 3HV monomer or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a maximum of 0.3 mol% of 3HHx, respectively. Fed-batch cultivation performed in a 10 L-scale bioreactor showed a maximum P(3HB-co-3HV) production of 9.9 g/L, which was 65.1% of total biomass and consisted of 21.8 mol% of 3HV monomer, with a maximum volumetric productivity of 0.22 g/(L h). The 3HV conversion yield achieved 1.04 g/g after 48 h cultivation, which was 76.7% of the maximum theoretical yield. B. glumae MA13 has showed to be an adapted bacterial strain to the synthesis of bioplastics from biofuel byproducts, and so from here it has been revealed as a promising PHA producer for an associated production set which has been considered as a prominent and ecologically friendly alternative to petrochemical plastics and fuels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fed Univ Tocantins, Palmas Campus,Av NS 15,Quadra 109 Norte, BR-77001090 Palmas, TO, BrazilSao Paulo State Univ, Inst Biosci, Av-24A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ, Bioenergy Res Inst, Av-24A,1515, BR-13506900 Rio Claro, SP, BrazilFed Univ Tocantins, Gurupi Campus,Chacaras 69-72, BR-77920090 Gurupi, TO, BrazilUniv Sao Paulo, Inst Biomed Sci, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP, BrazilSao Paulo State Univ, Inst Biosci, Av-24A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ, Bioenergy Res Inst, Av-24A,1515, BR-13506900 Rio Claro, SP, BrazilElsevier B.V.Fed Univ TocantinsUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Paula, Fabricio Coutinho deKakazu, Sergio [UNESP]Chimello de Paula, Carolina Bilia [UNESP]Almeida, Alex Fernando deCabrera Gomez, Jose GregorioContiero, Jonas [UNESP]2020-12-11T01:57:09Z2020-12-11T01:57:09Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.bcab.2019.101268Biocatalysis And Agricultural Biotechnology. Amsterdam: Elsevier, v. 20, 11 p., 2019.http://hdl.handle.net/11449/19753310.1016/j.bcab.2019.101268WOS:000493911600091Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocatalysis And Agricultural Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T04:53:42Zoai:repositorio.unesp.br:11449/197533Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:23:11.531310Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
title |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
spellingShingle |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol Paula, Fabricio Coutinho de Biodiesel Bioplastic Burkholderia glumae Glycerol Polyhydroxyalkanoates |
title_short |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
title_full |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
title_fullStr |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
title_full_unstemmed |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
title_sort |
Burkholderia glumae MA13: A newly isolated bacterial strain suitable for polyhydroxyalkanoate production from crude glycerol |
author |
Paula, Fabricio Coutinho de |
author_facet |
Paula, Fabricio Coutinho de Kakazu, Sergio [UNESP] Chimello de Paula, Carolina Bilia [UNESP] Almeida, Alex Fernando de Cabrera Gomez, Jose Gregorio Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Kakazu, Sergio [UNESP] Chimello de Paula, Carolina Bilia [UNESP] Almeida, Alex Fernando de Cabrera Gomez, Jose Gregorio Contiero, Jonas [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Fed Univ Tocantins Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Paula, Fabricio Coutinho de Kakazu, Sergio [UNESP] Chimello de Paula, Carolina Bilia [UNESP] Almeida, Alex Fernando de Cabrera Gomez, Jose Gregorio Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
Biodiesel Bioplastic Burkholderia glumae Glycerol Polyhydroxyalkanoates |
topic |
Biodiesel Bioplastic Burkholderia glumae Glycerol Polyhydroxyalkanoates |
description |
Burkholderia glumae MA13 was isolated from soil samples of Atlantic rain forest ecosystem as a new bacterial strain for polyhydroxyalkanoate (PHA) production from crude glycerol as sole carbon source. Among 107 glycerol consuming bacterial isolates, B. glumae MA13 was cultivated in shake flask experiments in order to verify its PHA production capability from crude glycerol besides waste cooking oil and sugarcane molasses free of pretreatment, showing intracellular poly(3-hydroxybutyrate) [P(3HB)] accumulation values of 51.3, 51.4 and 49% of cell dry weight (CDW), respectively. The addition of propionic or hexanoic acids to the media containing crude glycerol resulted in the copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] comprised of up to 32.1 mol% of 3HV monomer or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a maximum of 0.3 mol% of 3HHx, respectively. Fed-batch cultivation performed in a 10 L-scale bioreactor showed a maximum P(3HB-co-3HV) production of 9.9 g/L, which was 65.1% of total biomass and consisted of 21.8 mol% of 3HV monomer, with a maximum volumetric productivity of 0.22 g/(L h). The 3HV conversion yield achieved 1.04 g/g after 48 h cultivation, which was 76.7% of the maximum theoretical yield. B. glumae MA13 has showed to be an adapted bacterial strain to the synthesis of bioplastics from biofuel byproducts, and so from here it has been revealed as a promising PHA producer for an associated production set which has been considered as a prominent and ecologically friendly alternative to petrochemical plastics and fuels. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01 2020-12-11T01:57:09Z 2020-12-11T01:57:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bcab.2019.101268 Biocatalysis And Agricultural Biotechnology. Amsterdam: Elsevier, v. 20, 11 p., 2019. http://hdl.handle.net/11449/197533 10.1016/j.bcab.2019.101268 WOS:000493911600091 |
url |
http://dx.doi.org/10.1016/j.bcab.2019.101268 http://hdl.handle.net/11449/197533 |
identifier_str_mv |
Biocatalysis And Agricultural Biotechnology. Amsterdam: Elsevier, v. 20, 11 p., 2019. 10.1016/j.bcab.2019.101268 WOS:000493911600091 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biocatalysis And Agricultural Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129422609350656 |